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zeiss_lightsheet_z1

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Location: Room P3-A-3
Manufacturer: Carl Zeiss MicroImaging
Model: Lightsheet Z.1
Software: ZEN 2014 SP1
Year: 2016
SN: 2583000193
 
 
 
 
 
Zeiss Lightsheet Z1 Booking
Zeiss Lightsheet Z1 Usage Statistics

Microscope overview

The Zeiss Lightsheet Z1 is a light sheet fluorescence microscope able to image optical sections of large samples at subcellular resolution and very fast rates, with almost no phototoxicity or bleaching. It splits fluorescence excitation and detection into two separate light paths, with the axis of illumination being perpendicular to the detection axis. Since only a single thin section of the sample is illuminated by the light sheet, optical sectioning is achieved without a pinhole or image processing deconvolution. Light from the in-focus plane is collected by a sCMOS camera rather than pixel by pixel as in a point scanning laser confocal. This allows you to collect images faster and with less excitation light than you would with many other optical-sectioning microscopy techniques. You can image living samples in water or fixed samples where tissue clearing has been performed. Samples are mounted not on coverslips but inside special chambers that provide heating and cooling. The system is equipped with lasers from green to far red (488, 561 and 638 nm excitation wavelenghts) and appropriate fluorescence emission filters. With this system you can image live drosophila and zebrafish samples or use tissue clearing to perform optical sectioning deep into large fluorescent samples such as tissue sections, brains, embryos, organs, spheroids or biopsies. If you need higher resolution and have a smaller and thinner sample, check instead a confocal microscope such as the spinning disk 3i Marianas SDC, the line scanning Zeiss LSM 7 Live or the point scanners Zeiss LSM 880 and Zeiss LSM 710. A dedicated computer running arivis Vision4D is available for working with multi-channel 2D, 3D and 4D images you generate in the system. If your personal computer cannot handle all the data you collected, check out the Big Guy or Colossus.

System components

LASERs

Laser Unit Wavelength Maximum Power Current Status
Solid State 488 488 nm 30 mW ok
Solid State 561 561 nm 20 mW ok
Solid State 638 638 nm 75 mW ok

Objectives (Illumination)

Magnification Model NA
10x Lightsheet Z.1 10x 0.20

Objectives (Detection)

Magnification Model Type NA WD (mm)
20x W Plan-Apochromat Water 1.00 2.4
20x Clr Plan-Neofluar Corr nd=1.45 Clearing 1.00 5.6

Filtersets (Ocular)

Position Filterset Reference Excitation Emission
1 Green FS38HE 450-490 nm 500-550 nm
2 Red FS43 533-558 nm 570-640 nm
3 Blue FS49 G 365 nm 420-470 nm

Microscope Turn On Procedure

  • Turn on the MAIN SWITCH
  • Turn on the two switches: SYSTEMS/PC and COMPONENTS

zeiss_lsm_880_1.jpg

  • Turn the computer on
  • Login with your unit account
  • Wait for network icon to change to connected state
  • Start the ZEN software

Microscope Turn Off Procedure

If there's another user for this microscope in the next hour:

  • Close the software, leave the lasers on and log off the computer.
  • Clean up any objective where you used immersion oil.
  • Make sure that there really is another user going to use the microscope.

Else:

  • Turn off the lasers on ZEN
  • Clean up any objective were you used immersion oil.
  • Exit the software, shut down the computer.
  • Turn off the main switches SYSTEMS/PC and COMPONENTS
  • Wait 5 min. for Ar laser cooldown
  • Turn off the MAIN SWITCH.

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zeiss_lightsheet_z1.1460671718.txt.gz ยท Last modified: 2016/04/15 00:08 by bioimaging