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zeiss_cell_observer_wf [2016/06/08 17:33]
bioimaging [Objectives]
— (current)
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-<​html>​ 
-<img src="/​facility/​bioimaging/​lib/​exe/​fetch.php?​media=zeiss_cell_observer_wf_name.jpg">​ 
-<table style="​border:​0px solid white;">​ 
-<tr style="​border:​0px solid white;">​ 
-<td style="​border:​0px solid white;">​ 
-<a href="/​facility/​bioimaging/​lib/​exe/​fetch.php?​media=zeiss_cell_observer_wf_wiki.jpg"><​img src="/​facility/​bioimaging/​lib/​exe/​fetch.php?​media=zeiss_cell_observer_wf_wiki.jpg"​ width=300></​a>​ 
-<td style="​border:​0px solid white;">​ <p style="​line-height:​1.8">​ 
-<​b>​Location</​b>:​ <a href="/​facility/​bioimaging/​lib/​exe/​fetch.php?​media=zeiss_cell_observer_wf_map.jpg">​Room P3-A-10</​a>​ (<img src="/​facility/​bioimaging/​lib/​exe/​fetch.php?​media=telephone_icon.gif"​ width=15>​ 47311) <​br> ​ 
-<​b>​Manufacturer</​b>:​ <a href="​http://​www.zeiss.de/​micro"​ target="​_blank">​Carl Zeiss MicroImaging</​a><​br>​ 
-<​b>​Model</​b>:​ Cell Observer<​br>​ 
-<​b>​Software</​b>:​ ZEN 2 (blue edition)<​br>​ 
-<​b>​Year</​b>:​ 2015 <br> 
-<​b>​SN</​b>:​ 1026052056<​br>​ 
-&nbsp <br> 
-&nbsp <br> 
-&nbsp <br> 
-&nbsp <br> 
-&rarr; <a href="​https://​imm.medicina.ulisboa.pt/​intranet/​booking/​125"><​img src="/​facility/​bioimaging/​lib/​exe/​fetch.php?​media=date.png"> ​ Zeiss Cell Observer WF Booking</​a>​ 
- <​br> ​ 
-&rarr; <a href="/​facility/​bioimaging/​doku.php?​id=zeiss_cell_observer_wf_quality"><​img src="/​facility/​bioimaging/​lib/​exe/​fetch.php?​media=list-icon.png"​ width=18>​ Zeiss Cell Observer WF Quality Control</​a>​ 
- <​br>​ 
-&rarr; <a href="/​facility/​bioimaging/​doku.php?​id=zeiss_cell_observer_wf_usage"><​img src="/​facility/​bioimaging/​lib/​exe/​fetch.php?​media=chart_line.png"> ​ Zeiss Cell Observer WF Usage Statistics</​a>​ 
- </​p>​ 
-</td> 
-</tr> 
-</​table>​ 
-</​html>​ 
  
-===== Microscope overview ===== 
-The Zeiss Cell Observer WF is a fully motorized inverted //widefield fluorescence microscope//​ ideal for live-cell imaging applications. It is equipped with a large cage incubator and a small stage incubator for temperature control and CO2 supply and with Definite Focus for hardware focus control during long time-lapse acquisitions. Using the ZEN 2 software you can control the motorized filters, shutters and stage to simultaneously set up multi-color time-lapse imaging experiments in multiple stage positions. Its sensitive cooled Axiocam 506m camera allows you to capture weak fluorescent signals and minimize photobleaching/​photodamage in light sensitive samples. You can also perform Z-stack acquisition and use our [[http://​micro.magnet.fsu.edu/​primer/​digitalimaging/​deconvolution/​deconvolutionhome.html|deconvolution]] server [[brainstorm|Brainstorm]] to significantly improve image resolution even in thick samples. If don't want to use deconvolution,​ check out one of the confocal systems such as the [[zeiss_lsm_710|Zeiss LSM 710]], the [[zeiss_lsm_880|Zeiss LSM 880]] or the [[3i_marianas_sdc|3i Marianas SDC]] spinning disk. If your personal computer cannot handle all the data you collected, check out the [[big_guy|Big Guy]] or [[colossus|Colossus]]. If you have FISH or other fixed fluorescence slides try the [[leica_dm5000b|Leica DM5000B]] instead. 
- 
-===== Booking Rules ===== 
-The Zeiss Cell Observer WF booking is divided in three daily slots: 
-|  9:00 to 13:00  |  13:00 to 17:00  |  17:00 to 21:00  | 
- 
-  - A **user** can book at maximum **2 sessions per week** 
-  - Each **session must fit inside one of the three daily slots** (i.e. a session booked from 11:00 to 14:00 counts as 2 sessions) 
-  - The above rules do not apply for **weekends** and for work days **before 9:00** and **after 21:00** 
-  - The above rules do not apply for sessions booked on the very same day or **24 hours ahead** 
-  - Exceptions to these rules require **approval from [[joserino@medicina.ulisboa.pt|José Rino]]**. 
-  - Users must indicate which lasers they are going to use in the booking Description field 
-  - Users must indicate if they will use the incubator in the booking Description field (write down 37C INCUBATOR) 
-  - Users must write their internal extension phone number in the booking Description field 
- 
-===== System components ===== 
- 
-==== Filtersets ==== 
- 
-^  Position ​ ^  Filterset ​ ^  Reference ​ ^  Excitation ​ ^  Emission ​ ^ 
-|  1  |  HE GFP  |  [[https://​www.micro-shop.zeiss.com/?​s=23108437692794b&​l=en&​p=us&​f=f&​a=v&​b=f&​id=489038-9901-000&​o=|FS38HE]] ​ |  450-490 nm  |  500-550 nm  | 
-|  2  |  HE DsRed  |  [[https://​www.micro-shop.zeiss.com/?​s=23108437692794b&​l=en&​p=us&​f=f&​a=v&​b=f&​id=489043-9901-000&​o=|FS43HE]] ​ |  538-562 nm  |  570-640 nm  | 
-|  3  |  DAPI  |  [[https://​www.micro-shop.zeiss.com/?​s=23108437692794b&​l=en&​p=us&​f=f&​a=v&​b=f&​id=488049-9901-000&​o=|FS49]] ​ |  335-383 nm  |  420-470 nm  | 
-|  4  |  Cy5  |  [[https://​www.micro-shop.zeiss.com/?​s=23108437692794b&​l=en&​p=us&​f=f&​a=v&​b=f&​id=488050-9901-000&​o=|FS50]] ​ |  625-655 nm  |  665-715 nm  | 
-|  5  |  none  |  none  |  none  |  none  | 
-|  6  |  none  |  none  |  none  |  none  | 
- 
-==== Objectives ==== 
-^  Magnification ​ ^  Model  ^  Type  ^  NA  ^  WD (mm)  ^ 
-|  10x  |  [[https://​www.micro-shop.zeiss.com/?​s=3407786617d462&​l=en&​p=uk&​f=o&​a=v&​m=s&​id=420340-9901-000|EC Plan-Neofluar]] ​ |  Dry  |  0.30  |  5.2  | 
-|  20x  |  [[https://​www.micro-shop.zeiss.com/?​s=2310860526ae9cb&​l=en&​p=uk&​f=o&​a=v&​m=s&​id=420650-9901-000|Plan-Apochromat]] ​ |  Dry  |  0.80  |  0.55  | 
-|  40x  |  [[https://​www.micro-shop.zeiss.com/?​s=3407786617d462&​l=en&​p=uk&​f=o&​a=v&​m=s&​id=440350-9903-000|EC Plan-NeoFluar]] ​ |  Dry  |  0.75  |  0.71  | 
-|  63x  |  [[https://​www.micro-shop.zeiss.com/?​s=3407786617d462&​l=en&​p=uk&​f=o&​a=v&​m=s&​id=420782-9900-000|Plan-Apochromat]] ​ |  Oil  |  1.40  |  0.19  | 
- 
-**Upon request:** 
-^  Magnification ​ ^  Model  ^  Type  ^  NA  ^  WD (mm)  ^ 
-|  1.25x  |  [[https://​www.micro-shop.zeiss.com/?​s=3407786617d462&​l=en&​p=uk&​f=o&​a=v&​m=s&​id=422310-9900-000|EC Epiplan-Neofluar]] ​ |  Dry  |  0.035  |  4  | 
-|  5x  |  [[https://​www.micro-shop.zeiss.com/?​s=571974962233f6&​l=en&​p=uk&​f=o&​a=v&​m=s&​id=420330-9901-000|EC Plan-NeoFluar]] ​ |  Dry  |  0.16  |  18.5  | 
-|  20x  |  [[https://​www.micro-shop.zeiss.com/?​s=3407786617d462&​l=en&​p=uk&​f=o&​a=v&​m=s&​id=440341-9904-000|EC Plan-Neofluar Ph 2]]  |  Dry  |  0.50  |  2  | 
-|  40x  |  [[https://​www.micro-shop.zeiss.com/?​s=129353259d52f12&​l=en&​p=uk&​f=o&​a=v&​m=s&​id=420660-9970-000|Plan-Apochromat]] ​ |  Dry  |  0.95  |  0.25  | 
-|  63x  |  [[https://​www.micro-shop.zeiss.com/?​s=571858182d6d9d&​l=en&​p=uk&​f=o&​a=v&​m=s&​id=420781-9910-000|Plan-Apochromat Ph3]]  |  Oil  |  1.4  |  0.19  | 
-|  100x  |  [[https://​www.micro-shop.zeiss.com/?​s=7138707e1a1de&​l=en&​p=uk&​f=o&​a=v&​m=s&​id=420792-9800-000|alpha Plan-Apochromat]] ​ |  Oil  |  1.46  |  0.11  | 
- 
-===== System Turn On Procedures ===== 
-  * Turn on the HXP 120 fluorescence light. 
-{{zeiss_cell_observer_wf_hxp.jpg?​120|}} 
-  * Turn on the Temperature and CO2 controller (if you need them). 
-{{zeiss_cell_observer_wf_temp.jpg?​80|}} 
-  * If you need CO2, open the valve 
-{{zeiss_cell_observer_wf_co2.jpg?​80|}} 
-  * Turn on the Motorized Stage controller (A) 
-{{zeiss_cell_observer_wf_a.jpg?​height=150|}} 
-  * Turn on the Definite Focus controller (if you are going to use it) 
-{{zeiss_cell_observer_wf_df.jpg?​height=150|}} 
-  * Turn on the Microscope power supply (B) 
-{{zeiss_cell_observer_wf_b.jpg?​height=150|}} 
-  * Turn on the computer 
-  * Log in Windows with your lab user account (each lab has it's own area and login). 
-  * Start the **ZEN 2** software 
- 
-===== Microscope Turn Off procedures ===== 
-  * Exit the **ZEN 2** software 
-  * Turn off the computer 
-  * If you used any __immersion oil in the objectives, clean them up__. 
-  * Turn off the microscope power supply (B) 
-  * Turn off the Definite Focus controller (if used) 
-  * Turn off the Motorized Stage controller (A) 
-  * Turn off the Temperature and CO2 controller (if used) 
-  * If there'​s __another user__ for this microscope in the __next hour__ leave the mercury __lamp power source on, else turn it off__. ​ 
-  * Be extra careful in weekends and at the end of the day to make sure that the mercury lamp is not going to be turned on for an excessive time.  
- 
----- 
-[[resources|Back to the Resources page]] 
zeiss_cell_observer_wf.1465400015.txt.gz · Last modified: 2016/06/08 17:33 by bioimaging