zeiss_cell_observer_wf
Differences
This shows you the differences between two versions of the page.
| Both sides previous revision Previous revision Next revision | Previous revision Next revision Both sides next revision | ||
|
zeiss_cell_observer_wf [2016/04/06 00:21] bioimaging |
zeiss_cell_observer_wf [2016/06/29 10:19] bioimaging |
||
|---|---|---|---|
| Line 8: | Line 8: | ||
| <b>Location</b>: <a href="/facility/bioimaging/lib/exe/fetch.php?media=zeiss_cell_observer_wf_map.jpg">Room P3-A-10</a> (<img src="/facility/bioimaging/lib/exe/fetch.php?media=telephone_icon.gif" width=15> 47311) <br> | <b>Location</b>: <a href="/facility/bioimaging/lib/exe/fetch.php?media=zeiss_cell_observer_wf_map.jpg">Room P3-A-10</a> (<img src="/facility/bioimaging/lib/exe/fetch.php?media=telephone_icon.gif" width=15> 47311) <br> | ||
| <b>Manufacturer</b>: <a href="http://www.zeiss.de/micro" target="_blank">Carl Zeiss MicroImaging</a><br> | <b>Manufacturer</b>: <a href="http://www.zeiss.de/micro" target="_blank">Carl Zeiss MicroImaging</a><br> | ||
| - | <b>Model</b>: Cell Observer<br> | + | <b>Model</b>: Axio Observer<br> |
| <b>Software</b>: ZEN 2 (blue edition)<br> | <b>Software</b>: ZEN 2 (blue edition)<br> | ||
| <b>Year</b>: 2015 <br> | <b>Year</b>: 2015 <br> | ||
| Line 16: | Line 16: | ||
|   <br> |   <br> | ||
|   <br> |   <br> | ||
| - | → <a href="https://imm.medicina.ulisboa.pt/intranet/booking/month.php?user=ZEISSCELL1&rid=125"><img src="/facility/bioimaging/lib/exe/fetch.php?media=date.png"> Zeiss Cell Observer WF Booking</a> | + | → <a href="https://imm.medicina.ulisboa.pt/intranet/booking/125"><img src="/facility/bioimaging/lib/exe/fetch.php?media=date.png"> Zeiss Cell Observer WF Booking</a> |
| <br> | <br> | ||
| → <a href="/facility/bioimaging/doku.php?id=zeiss_cell_observer_wf_quality"><img src="/facility/bioimaging/lib/exe/fetch.php?media=list-icon.png" width=18> Zeiss Cell Observer WF Quality Control</a> | → <a href="/facility/bioimaging/doku.php?id=zeiss_cell_observer_wf_quality"><img src="/facility/bioimaging/lib/exe/fetch.php?media=list-icon.png" width=18> Zeiss Cell Observer WF Quality Control</a> | ||
| Line 32: | Line 32: | ||
| ===== Booking Rules ===== | ===== Booking Rules ===== | ||
| The Zeiss Cell Observer WF booking is divided in three daily slots: | The Zeiss Cell Observer WF booking is divided in three daily slots: | ||
| - | |9:00 to 13:00|13:00 to 17:00|17:00 to 21:00| | + | | 9:00 to 13:00 | 13:00 to 17:00 | 17:00 to 21:00 | |
| - A **user** can book at maximum **2 sessions per week** | - A **user** can book at maximum **2 sessions per week** | ||
| Line 59: | Line 59: | ||
| | 10x | [[https://www.micro-shop.zeiss.com/?s=3407786617d462&l=en&p=uk&f=o&a=v&m=s&id=420340-9901-000|EC Plan-Neofluar]] | Dry | 0.30 | 5.2 | | | 10x | [[https://www.micro-shop.zeiss.com/?s=3407786617d462&l=en&p=uk&f=o&a=v&m=s&id=420340-9901-000|EC Plan-Neofluar]] | Dry | 0.30 | 5.2 | | ||
| | 20x | [[https://www.micro-shop.zeiss.com/?s=2310860526ae9cb&l=en&p=uk&f=o&a=v&m=s&id=420650-9901-000|Plan-Apochromat]] | Dry | 0.80 | 0.55 | | | 20x | [[https://www.micro-shop.zeiss.com/?s=2310860526ae9cb&l=en&p=uk&f=o&a=v&m=s&id=420650-9901-000|Plan-Apochromat]] | Dry | 0.80 | 0.55 | | ||
| - | | 20x | [[https://www.micro-shop.zeiss.com/?s=3407786617d462&l=en&p=uk&f=o&a=v&m=s&id=440341-9904-000|EC Plan-Neofluar Ph 2]] | Dry | 0.50 | 2.0 | | ||
| | 40x | [[https://www.micro-shop.zeiss.com/?s=3407786617d462&l=en&p=uk&f=o&a=v&m=s&id=440350-9903-000|EC Plan-NeoFluar]] | Dry | 0.75 | 0.71 | | | 40x | [[https://www.micro-shop.zeiss.com/?s=3407786617d462&l=en&p=uk&f=o&a=v&m=s&id=440350-9903-000|EC Plan-NeoFluar]] | Dry | 0.75 | 0.71 | | ||
| | 63x | [[https://www.micro-shop.zeiss.com/?s=3407786617d462&l=en&p=uk&f=o&a=v&m=s&id=420782-9900-000|Plan-Apochromat]] | Oil | 1.40 | 0.19 | | | 63x | [[https://www.micro-shop.zeiss.com/?s=3407786617d462&l=en&p=uk&f=o&a=v&m=s&id=420782-9900-000|Plan-Apochromat]] | Oil | 1.40 | 0.19 | | ||
| - | | 100x | [[https://www.micro-shop.zeiss.com/?s=3407786617d462&l=en&p=uk&f=o&a=v&m=s&id=440782-9902-000|Plan-Apochromat]] | Oil | 1.40 | 0.17 | | ||
| **Upon request:** | **Upon request:** | ||
| Line 68: | Line 66: | ||
| | 1.25x | [[https://www.micro-shop.zeiss.com/?s=3407786617d462&l=en&p=uk&f=o&a=v&m=s&id=422310-9900-000|EC Epiplan-Neofluar]] | Dry | 0.035 | 4 | | | 1.25x | [[https://www.micro-shop.zeiss.com/?s=3407786617d462&l=en&p=uk&f=o&a=v&m=s&id=422310-9900-000|EC Epiplan-Neofluar]] | Dry | 0.035 | 4 | | ||
| | 5x | [[https://www.micro-shop.zeiss.com/?s=571974962233f6&l=en&p=uk&f=o&a=v&m=s&id=420330-9901-000|EC Plan-NeoFluar]] | Dry | 0.16 | 18.5 | | | 5x | [[https://www.micro-shop.zeiss.com/?s=571974962233f6&l=en&p=uk&f=o&a=v&m=s&id=420330-9901-000|EC Plan-NeoFluar]] | Dry | 0.16 | 18.5 | | ||
| - | | 63x (Ph 3) | [[https://www.micro-shop.zeiss.com/?s=571858182d6d9d&l=en&p=uk&f=o&a=v&m=s&id=420781-9910-000|Plan-Apochromat]] | Oil | 1.4 | 0.19 | | + | | 20x | [[https://www.micro-shop.zeiss.com/?s=3407786617d462&l=en&p=uk&f=o&a=v&m=s&id=440341-9904-000|EC Plan-Neofluar Ph 2]] | Dry | 0.50 | 2 | |
| + | | 40x | [[https://www.micro-shop.zeiss.com/?s=129353259d52f12&l=en&p=uk&f=o&a=v&m=s&id=420660-9970-000|Plan-Apochromat]] | Dry | 0.95 | 0.25 | | ||
| + | | 40x | [[https://www.micro-shop.zeiss.com/?s=3407786617d462&l=en&p=uk&f=o&a=v&m=s&id=440451-9903-000|Plan-Neofluar Ph3]] | Oil | 1.30 | 0.20 | | ||
| + | | 63x | [[https://www.micro-shop.zeiss.com/?s=571858182d6d9d&l=en&p=uk&f=o&a=v&m=s&id=420781-9910-000|Plan-Apochromat Ph3]] | Oil | 1.4 | 0.19 | | ||
| + | | 100x | [[https://www.micro-shop.zeiss.com/?s=7138707e1a1de&l=en&p=uk&f=o&a=v&m=s&id=420792-9800-000|alpha Plan-Apochromat]] | Oil | 1.46 | 0.11 | | ||
| ===== System Turn On Procedures ===== | ===== System Turn On Procedures ===== | ||
| * Turn on the HXP 120 fluorescence light. | * Turn on the HXP 120 fluorescence light. | ||
| - | {{:bioimaging:zeiss_cell_observer_wf_hxp.jpg?120|}} | + | {{zeiss_cell_observer_wf_hxp.jpg?120|}} |
| * Turn on the Temperature and CO2 controller (if you need them). | * Turn on the Temperature and CO2 controller (if you need them). | ||
| - | {{:bioimaging:zeiss_cell_observer_wf_temp.jpg?80|}} | + | {{zeiss_cell_observer_wf_temp.jpg?80|}} |
| * If you need CO2, open the valve | * If you need CO2, open the valve | ||
| - | {{:bioimaging:zeiss_cell_observer_wf_co2.jpg?80|}} | + | {{zeiss_cell_observer_wf_co2.jpg?80|}} |
| * Turn on the Motorized Stage controller (A) | * Turn on the Motorized Stage controller (A) | ||
| - | {{:bioimaging:zeiss_cell_observer_wf_a.jpg?height=150|}} | + | {{zeiss_cell_observer_wf_a.jpg?height=150|}} |
| * Turn on the Definite Focus controller (if you are going to use it) | * Turn on the Definite Focus controller (if you are going to use it) | ||
| - | {{:bioimaging:zeiss_cell_observer_wf_df.jpg?height=150|}} | + | {{zeiss_cell_observer_wf_df.jpg?height=150|}} |
| * Turn on the Microscope power supply (B) | * Turn on the Microscope power supply (B) | ||
| - | {{:bioimaging:zeiss_cell_observer_wf_b.jpg?height=150|}} | + | {{zeiss_cell_observer_wf_b.jpg?height=150|}} |
| * Turn on the computer | * Turn on the computer | ||
| * Log in Windows with your lab user account (each lab has it's own area and login). | * Log in Windows with your lab user account (each lab has it's own area and login). | ||
| - | * Start the ZEN 2 software | + | * Start the **ZEN 2** software |
| ===== Microscope Turn Off procedures ===== | ===== Microscope Turn Off procedures ===== | ||
| - | * Exit the ZEN 2 software | + | * Exit the **ZEN 2** software |
| * Turn off the computer | * Turn off the computer | ||
| * If you used any __immersion oil in the objectives, clean them up__. | * If you used any __immersion oil in the objectives, clean them up__. | ||
| Line 100: | Line 101: | ||
| ---- | ---- | ||
| - | [[groups:bioimage:resources|Back to the Resources page]] | + | [[resources|Back to the Resources page]] |
Page Tools
