zeiss_cell_observer_wf
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zeiss_cell_observer_wf [2016/04/05 16:26] bioimaging |
zeiss_cell_observer_wf [2016/06/29 10:19] bioimaging |
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| <b>Location</b>: <a href="/facility/bioimaging/lib/exe/fetch.php?media=zeiss_cell_observer_wf_map.jpg">Room P3-A-10</a> (<img src="/facility/bioimaging/lib/exe/fetch.php?media=telephone_icon.gif" width=15> 47311) <br> | <b>Location</b>: <a href="/facility/bioimaging/lib/exe/fetch.php?media=zeiss_cell_observer_wf_map.jpg">Room P3-A-10</a> (<img src="/facility/bioimaging/lib/exe/fetch.php?media=telephone_icon.gif" width=15> 47311) <br> | ||
| <b>Manufacturer</b>: <a href="http://www.zeiss.de/micro" target="_blank">Carl Zeiss MicroImaging</a><br> | <b>Manufacturer</b>: <a href="http://www.zeiss.de/micro" target="_blank">Carl Zeiss MicroImaging</a><br> | ||
| - | <b>Model</b>: Cell Observer<br> | + | <b>Model</b>: Axio Observer<br> |
| <b>Software</b>: ZEN 2 (blue edition)<br> | <b>Software</b>: ZEN 2 (blue edition)<br> | ||
| <b>Year</b>: 2015 <br> | <b>Year</b>: 2015 <br> | ||
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|   <br> |   <br> | ||
|   <br> |   <br> | ||
| - | → <a href="https://imm.medicina.ulisboa.pt/intranet/booking/month.php?user=ZEISSCELL1&rid=125"><img src="/facility/bioimaging/lib/exe/fetch.php?media=date.png"> Zeiss Cell Observer WF Booking</a> | + | → <a href="https://imm.medicina.ulisboa.pt/intranet/booking/125"><img src="/facility/bioimaging/lib/exe/fetch.php?media=date.png"> Zeiss Cell Observer WF Booking</a> |
| <br> | <br> | ||
| → <a href="/facility/bioimaging/doku.php?id=zeiss_cell_observer_wf_quality"><img src="/facility/bioimaging/lib/exe/fetch.php?media=list-icon.png" width=18> Zeiss Cell Observer WF Quality Control</a> | → <a href="/facility/bioimaging/doku.php?id=zeiss_cell_observer_wf_quality"><img src="/facility/bioimaging/lib/exe/fetch.php?media=list-icon.png" width=18> Zeiss Cell Observer WF Quality Control</a> | ||
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| </html> | </html> | ||
| + | ===== Microscope overview ===== | ||
| + | The Zeiss Cell Observer WF is a fully motorized inverted //widefield fluorescence microscope// ideal for live-cell imaging applications. It is equipped with a large cage incubator and a small stage incubator for temperature control and CO2 supply and with Definite Focus for hardware focus control during long time-lapse acquisitions. Using the ZEN 2 software you can control the motorized filters, shutters and stage to simultaneously set up multi-color time-lapse imaging experiments in multiple stage positions. Its sensitive cooled Axiocam 506m camera allows you to capture weak fluorescent signals and minimize photobleaching/photodamage in light sensitive samples. You can also perform Z-stack acquisition and use our [[http://micro.magnet.fsu.edu/primer/digitalimaging/deconvolution/deconvolutionhome.html|deconvolution]] server [[brainstorm|Brainstorm]] to significantly improve image resolution even in thick samples. If don't want to use deconvolution, check out one of the confocal systems such as the [[zeiss_lsm_710|Zeiss LSM 710]], the [[zeiss_lsm_880|Zeiss LSM 880]] or the [[3i_marianas_sdc|3i Marianas SDC]] spinning disk. If your personal computer cannot handle all the data you collected, check out the [[big_guy|Big Guy]] or [[colossus|Colossus]]. If you have FISH or other fixed fluorescence slides try the [[leica_dm5000b|Leica DM5000B]] instead. | ||
| + | |||
| + | ===== Booking Rules ===== | ||
| + | The Zeiss Cell Observer WF booking is divided in three daily slots: | ||
| + | | 9:00 to 13:00 | 13:00 to 17:00 | 17:00 to 21:00 | | ||
| + | |||
| + | - A **user** can book at maximum **2 sessions per week** | ||
| + | - Each **session must fit inside one of the three daily slots** (i.e. a session booked from 11:00 to 14:00 counts as 2 sessions) | ||
| + | - The above rules do not apply for **weekends** and for work days **before 9:00** and **after 21:00** | ||
| + | - The above rules do not apply for sessions booked on the very same day or **24 hours ahead** | ||
| + | - Exceptions to these rules require **approval from [[joserino@medicina.ulisboa.pt|José Rino]]**. | ||
| + | - Users must indicate which lasers they are going to use in the booking Description field | ||
| + | - Users must indicate if they will use the incubator in the booking Description field (write down 37C INCUBATOR) | ||
| + | - Users must write their internal extension phone number in the booking Description field | ||
| + | |||
| + | ===== System components ===== | ||
| + | |||
| + | ==== Filtersets ==== | ||
| + | |||
| + | ^ Position ^ Filterset ^ Reference ^ Excitation ^ Emission ^ | ||
| + | | 1 | HE GFP | [[https://www.micro-shop.zeiss.com/?s=23108437692794b&l=en&p=us&f=f&a=v&b=f&id=489038-9901-000&o=|FS38HE]] | 450-490 nm | 500-550 nm | | ||
| + | | 2 | HE DsRed | [[https://www.micro-shop.zeiss.com/?s=23108437692794b&l=en&p=us&f=f&a=v&b=f&id=489043-9901-000&o=|FS43HE]] | 538-562 nm | 570-640 nm | | ||
| + | | 3 | DAPI | [[https://www.micro-shop.zeiss.com/?s=23108437692794b&l=en&p=us&f=f&a=v&b=f&id=488049-9901-000&o=|FS49]] | 335-383 nm | 420-470 nm | | ||
| + | | 4 | Cy5 | [[https://www.micro-shop.zeiss.com/?s=23108437692794b&l=en&p=us&f=f&a=v&b=f&id=488050-9901-000&o=|FS50]] | 625-655 nm | 665-715 nm | | ||
| + | | 5 | none | none | none | none | | ||
| + | | 6 | none | none | none | none | | ||
| + | |||
| + | ==== Objectives ==== | ||
| + | ^ Magnification ^ Model ^ Type ^ NA ^ WD (mm) ^ | ||
| + | | 10x | [[https://www.micro-shop.zeiss.com/?s=3407786617d462&l=en&p=uk&f=o&a=v&m=s&id=420340-9901-000|EC Plan-Neofluar]] | Dry | 0.30 | 5.2 | | ||
| + | | 20x | [[https://www.micro-shop.zeiss.com/?s=2310860526ae9cb&l=en&p=uk&f=o&a=v&m=s&id=420650-9901-000|Plan-Apochromat]] | Dry | 0.80 | 0.55 | | ||
| + | | 40x | [[https://www.micro-shop.zeiss.com/?s=3407786617d462&l=en&p=uk&f=o&a=v&m=s&id=440350-9903-000|EC Plan-NeoFluar]] | Dry | 0.75 | 0.71 | | ||
| + | | 63x | [[https://www.micro-shop.zeiss.com/?s=3407786617d462&l=en&p=uk&f=o&a=v&m=s&id=420782-9900-000|Plan-Apochromat]] | Oil | 1.40 | 0.19 | | ||
| + | |||
| + | **Upon request:** | ||
| + | ^ Magnification ^ Model ^ Type ^ NA ^ WD (mm) ^ | ||
| + | | 1.25x | [[https://www.micro-shop.zeiss.com/?s=3407786617d462&l=en&p=uk&f=o&a=v&m=s&id=422310-9900-000|EC Epiplan-Neofluar]] | Dry | 0.035 | 4 | | ||
| + | | 5x | [[https://www.micro-shop.zeiss.com/?s=571974962233f6&l=en&p=uk&f=o&a=v&m=s&id=420330-9901-000|EC Plan-NeoFluar]] | Dry | 0.16 | 18.5 | | ||
| + | | 20x | [[https://www.micro-shop.zeiss.com/?s=3407786617d462&l=en&p=uk&f=o&a=v&m=s&id=440341-9904-000|EC Plan-Neofluar Ph 2]] | Dry | 0.50 | 2 | | ||
| + | | 40x | [[https://www.micro-shop.zeiss.com/?s=129353259d52f12&l=en&p=uk&f=o&a=v&m=s&id=420660-9970-000|Plan-Apochromat]] | Dry | 0.95 | 0.25 | | ||
| + | | 40x | [[https://www.micro-shop.zeiss.com/?s=3407786617d462&l=en&p=uk&f=o&a=v&m=s&id=440451-9903-000|Plan-Neofluar Ph3]] | Oil | 1.30 | 0.20 | | ||
| + | | 63x | [[https://www.micro-shop.zeiss.com/?s=571858182d6d9d&l=en&p=uk&f=o&a=v&m=s&id=420781-9910-000|Plan-Apochromat Ph3]] | Oil | 1.4 | 0.19 | | ||
| + | | 100x | [[https://www.micro-shop.zeiss.com/?s=7138707e1a1de&l=en&p=uk&f=o&a=v&m=s&id=420792-9800-000|alpha Plan-Apochromat]] | Oil | 1.46 | 0.11 | | ||
| + | |||
| + | ===== System Turn On Procedures ===== | ||
| + | * Turn on the HXP 120 fluorescence light. | ||
| + | {{zeiss_cell_observer_wf_hxp.jpg?120|}} | ||
| + | * Turn on the Temperature and CO2 controller (if you need them). | ||
| + | {{zeiss_cell_observer_wf_temp.jpg?80|}} | ||
| + | * If you need CO2, open the valve | ||
| + | {{zeiss_cell_observer_wf_co2.jpg?80|}} | ||
| + | * Turn on the Motorized Stage controller (A) | ||
| + | {{zeiss_cell_observer_wf_a.jpg?height=150|}} | ||
| + | * Turn on the Definite Focus controller (if you are going to use it) | ||
| + | {{zeiss_cell_observer_wf_df.jpg?height=150|}} | ||
| + | * Turn on the Microscope power supply (B) | ||
| + | {{zeiss_cell_observer_wf_b.jpg?height=150|}} | ||
| + | * Turn on the computer | ||
| + | * Log in Windows with your lab user account (each lab has it's own area and login). | ||
| + | * Start the **ZEN 2** software | ||
| + | |||
| + | ===== Microscope Turn Off procedures ===== | ||
| + | * Exit the **ZEN 2** software | ||
| + | * Turn off the computer | ||
| + | * If you used any __immersion oil in the objectives, clean them up__. | ||
| + | * Turn off the microscope power supply (B) | ||
| + | * Turn off the Definite Focus controller (if used) | ||
| + | * Turn off the Motorized Stage controller (A) | ||
| + | * Turn off the Temperature and CO2 controller (if used) | ||
| + | * If there's __another user__ for this microscope in the __next hour__ leave the mercury __lamp power source on, else turn it off__. | ||
| + | * Be extra careful in weekends and at the end of the day to make sure that the mercury lamp is not going to be turned on for an excessive time. | ||
| + | |||
| + | ---- | ||
| + | [[resources|Back to the Resources page]] | ||
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