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zeiss_cell_observer_sd

Location: Room P2-A-22 ( 47217)
Manufacturer: Carl Zeiss Microimaging
Model: Cell Observer SD
Nickname: "Cell Observer SD"
Software: ZEN 2.6 (blue edition)
Year: 2015
SN: 3851001637
 
 
 
Zeiss Cell Observer SD Booking
Zeiss Cell Observer SD Quality Control
Zeiss Cell Observer SD Usage Statistics  


System overview

The Zeiss Cell Observer SD spinning disk confocal microscope is a fast imaging system which provides a trade-off between confocality, resolution and speed. It is an inverted microscope ideal for live cell applications which require fast acquisition speeds rather than high resolution images. The scanning unit achieves confocality by directing light through a spinning disk with many small pinholes. Images are then acquired with a sensitive EMCCD which allows for very small exposure times but is limited in resolution to 512×512 pixels. The stage is motorized and furthermore equipped with a piezo for Z displacement so fast 4D imaging is possible in multiple stage positions. The system is also equipped with a large size incubator for temperature control, a small stage incubator for CO2 supply and with Definite Focus.2 for hardware focus control during large tile or multi-position/time-lapse acquisitions (check tutorial videos below). You can also use the system as a widefield microscope, using a sCMOS camera for acquisition. Even though its resolution is not as high as a point-scanning confocal system such as the Zeiss LSM 710, it is much faster. You can also check the 3i Marianas SDC if you need a spinning disk with higher laser power illumination. If your personal computer cannot handle all the data you collected, check out the Big Guy or Colossus.

Data files older than 1 month will be automatically deleted on this system, please copy your data to the iMM server using the desktop link.

Booking Rules

  1. Users can book at maximum 8 hours per week
  2. This usage restriction does not apply for weekends and for working days before 9:00 and after 21:00
  3. Exceptions to these rules require approval from José Rino.

System components

LASERs

Laser Unit Wavelength Maximum Power Current Status
Solid State Laser 405 405 nm 50 mW ok
Solid State Laser 488 488 nm 100 mW ok
Solid State Laser 561 561 nm 75 mW ok
Solid State Laser 638 638 nm 75 mW ok

Objectives

Magnification Model Immersion NA WD (mm) Reference
10x EC Plan-Neofluar Ph1 Air 0.30 5.20 420341-9911-000
20x Plan-Apochromat Ph2 Air 0.80 0.55 420651-9911-000
40x LD C-Apochromat Corr Water 1.10 0.62 421867-9970-000
40x Plan-Apochromat DIC Oil 1.40 0.13 420762-9900-000
63x Plan-Apochromat DIC Oil 1.40 0.19 420782-9900-000
100x α Plan-Apochromat DIC Oil 1.46 0.11 420792-9800-000

Upon request:

Magnification Model Immersion NA WD (mm) Reference
10x EC Plan-Neofluar Air 0.30 5.20 420340-9901-000
25x LCI Plan-Neofluar Corr DIC Oil/Glyc/W 0.80 0.21 420852-9972-000

Filtersets (Widefield)

Position Filterset Reference Excitation Dichroic Emission
1 Green FS38HE 450-490 nm 495 nm 500-550 nm
2 Red FS43HE 538-562 nm 570 nm 570-640 nm
3 Blue FS49 335-383 nm 395 nm 420-470 nm
4 Far Red FS50 625-655 nm 660 nm 665-715 nm
5 QUAD FS81HE 357-393 nm 400 nm 407-412 nm
473-496 nm 503 nm 511-528 nm
545-562 nm 570 nm 577-602 nm
620-642 nm 650 nm 658-704 nm
6 Empty

Emission Filters (Confocal)

Position Transmission
1: BP 450/50 425-475 nm
2: TBP 526 601 688 510-542 nm
586-616 nm
663-713 nm
3: none
4: FE01-520/35 502-537 nm
5: BP 600 50 575-625 nm
6: BP 690/50 665-715 nm

Cameras

Model Type Frame Size Pixel Size (µm) Quantum Efficiency
Photometrics Evolve 512 EMCCD 512 x 512 16 x 16 > 90%
Hamamatsu ORCA-flash4.0 V2 sCMOS 2048 x 2048 6.5 x 6.5 82%

Microscope Turn On Procedure

  • Turn on the computer
  • Login in to Windows (Bioimaging User)
  • Turn on the black socket outlets underneath the microscope table by order (1→2→3)

  • Wait 2 min to allow for system components detection
  • Start the ZEN Blue software

Warning: If you need to change the stage adapter, please contact the Bioimaging Unit (imm-bioimaging@medicina.ulisboa.pt | 47222)

Microscope Turn Off Procedure

If there is another user for this microscope in the next hour:

  • Exit the ZEN software, leave the lasers on
  • Log off the computer
  • Clean up immersion objectives

Else:

  • Exit the ZEN software
  • Shut down the computer
  • Clean up immersion objectives
  • Turn off the black socket outlets underneath the microscope table by reverse order (3→2→1)

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zeiss_cell_observer_sd.txt · Last modified: 2020/10/27 21:11 by bioimaging