Bioimaging Wiki

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<a href="/facility/bioimaging/lib/exe/fetch.php?media=zeiss_cell_observer_sd_wiki.jpg"><img src="/facility/bioimaging/lib/exe/fetch.php?media=zeiss_cell_observer_sd_wiki.jpg" width=300></a>
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<b>Location</b>: Room P2-A-22 (<img src="/facility/bioimaging/lib/exe/fetch.php?media=telephone_icon.gif" width=15> 47217) <br> 
<b>Manufacturer</b>: <a href="http://www.zeiss.de/micro" target="_blank">Carl Zeiss Microimaging</a><br>
<b>Model</b>: <a href="http://www.zeiss.com/microscopy/en_de/products/confocal-microscopes/cell-observer-sd-for-biology.html" target="_blank">Cell Observer SD</a><br>
<b>Nickname</b>: "Cell Observer SD"<br>
<b>Software</b>: ZEN<br>
<b>Year</b>: 2015 <br>
<b>SN</b>: 3851001637 <br>
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&rarr; <a href="https://imm.medicina.ulisboa.pt/intranet/booking/119"><img src="/facility/bioimaging/lib/exe/fetch.php?media=date.png">  Zeiss Cell Observer SD Booking</a>
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&rarr; <a href="/facility/bioimaging/doku.php?id=zeiss_cell_observer_sd_quality"><img src="/facility/bioimaging/lib/exe/fetch.php?media=list-icon.png" width=18> Zeiss Cell Observer SD Quality Control</a>
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&rarr; <a href="/facility/bioimaging/doku.php?id=zeiss_cell_observer_sd_usage"><img src="/facility/bioimaging/lib/exe/fetch.php?media=chart_line.png">  Zeiss Cell Observer SD Usage Statistics</a>
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===== System overview =====

{{  :spinning_disk.jpg?0x300|}} The Zeiss Cell Observer SD //spinning disk confocal microscope// is a fast imaging system which provides a trade-off between confocality, resolution and speed. It is an inverted microscope ideal for live cell applications which require fast acquisition speeds rather than high resolution images. The scanning unit achieves confocality by directing light through a spinning disk with many small pinholes. Images are then acquired with a sensitive EMCCD which allows for very small exposure times but is limited in resolution to 512x512 pixels. The stage is motorized and furthermore equipped with a piezo for Z displacement so fast 4D imaging is possible in multiple stage positions. The system is also equipped with a large size incubator for temperature control, a small stage incubator for CO2 supply and with Definite Focus for hardware focus control during long time-lapse acquisitions. You can also use the system as a widefield microscope, using a sCMOS camera for acquisition. Even though its resolution is not as high as a //point-scanning confocal system// such as the [[zeiss_lsm_880|Zeiss LSM 880]] or [[zeiss_lsm_710|Zeiss LSM 710]], it is much faster than these two systems. You can also check the [[3i_marianas_sdc|3i Marianas SDC]] if you need a spinning disk with higher laser power illumination. If your personal computer cannot handle all the data you collected, check out the [[big_guy|Big Guy]] or [[colossus|Colossus]].

  * **Microscope**: [[http://www.zeiss.com/microscopy/en_de/products/light-microscopes/axio-observer-for-biology.html|Zeiss Axio Observer]]
  * **Confocal scanner**: [[http://www.andor.com/microscopy_systems/peripherals/confocal_scanners/|Yokogawa CSU-x1]]
  * **EMCCD Camera**: [[http://www.photometrics.com/products/emccdcams/evolve/512.php|Evolve 512 EMCCD]]
  * **sCMOS Camera**: [[http://www.hamamatsu.com/eu/en/community/life_science_camera/product/search/C11440-22CU/index.html|Hamamatsu ORCA-flash4.0 V2]]

{{warning.jpg?20|}} Data files older than **1 month** will be automatically deleted on this system, please copy your data to the iMM server using the desktop link.

===== System components =====

==== LASERs ====
^  Laser Unit  ^  Wavelength  ^  Maximum Power  ^  Current Status  ^
|  Solid State Laser 405  |  405 nm  |  50 mW  |  ok  |
|  Solid State Laser 488  |  488 nm  |  100 mW  |  ok  |
|  Solid State Laser 561  |  561 nm  |  75 mW  |  ok  |
|  Solid State Laser 638  |  638 nm  |  75 mW  |  ok  |

==== Objectives ====

{{:zeiss_cell_observer_sd_objectives.jpg  |}}
^  Magnification   ^  Model  ^  Type  ^  NA  ^  WD (mm)  ^
|  20x  |  [[https://www.micro-shop.zeiss.com/?s=42373720ae8af6&l=en&p=us&f=o&a=v&m=s&id=420651-9911-000|Plan-Apochromat]]  |  Dry  |  0.8  |  0.55  |
|  40x  |  [[https://www.micro-shop.zeiss.com/?s=42373720ae8af6&l=en&p=us&f=o&a=v&m=s&id=421867-9970-000|LD C-Apochromat]]  |  Water  |  1.10  |  0.62  |
|  40x  |  [[https://www.micro-shop.zeiss.com/?s=42373720ae8af6&l=en&p=us&f=o&a=v&m=s&id=420762-9900-000|Plan-Apochromat]]  |  Oil  |  1.40  |  0.13  |
|  63x  |  [[https://www.micro-shop.zeiss.com/?s=3407786617d462&l=en&p=uk&f=o&a=v&m=s&id=420782-9900-000|Plan-Apochromat]]  |  Oil  |  1.40  |  0.19  |
|  63x  |  [[https://www.micro-shop.zeiss.com/?s=4238899732b41f&l=en&p=us&f=o&a=v&m=s&id=420781-9910-000|Plan-Apochromat Ph3]]  |  Oil  |  1.40  |  0.19  |
|  100x  |  [[https://www.micro-shop.zeiss.com/?s=7138707e1a1de&l=en&p=uk&f=o&a=v&m=s&id=420792-9800-000|alpha Plan-Apochromat]]  |  Oil  |  1.46  |  0.11  |

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==== Filtersets (Widefield) ====

{{:zeiss_cell_observer_sd_filters_widefield.jpg  |}}

^  Position  ^  Filterset  ^  Reference  ^  Excitation  ^  Emission  ^
|  1  |  Green  |  [[https://www.micro-shop.zeiss.com/?s=7139069fdc79c&l=en&p=us&f=f&a=v&b=f&id=489038-9901-000&o=|FS38HE]]  |  450-490 nm  |  500-550 nm  |
|  2  |  Red  |  [[https://www.micro-shop.zeiss.com/?s=4237481102cf7b&l=en&p=us&f=f&a=v&b=f&id=489043-9901-000&o=|FS43HE]]  |  538-562 nm  |  570-640 nm  |
|  3  |  Blue  |  [[https://www.micro-shop.zeiss.com/?s=163176628fcf844&l=en&p=us&f=f&a=v&b=f&id=488049-9901-000&o=|FS49]]  |  335-383 nm  |  420-470 nm  |
|  4  |  Far Red  |  [[https://www.micro-shop.zeiss.com/?s=4237481102cf7b&l=en&p=us&f=f&a=v&b=f&id=488050-9901-000&o=|FS50]]  |  625-655 nm  |  665-715 nm  |
|  5  |  QUAD  |  [[https://www.micro-shop.zeiss.com/?s=4237481102cf7b&l=en&p=us&f=f&a=v&b=f&id=489081-9010-000&o=|  FS81HE]]  |  357-393 nm  |  407-412 nm  |
|  :::  |  :::  |  :::  |  473-496 nm  |  511-528 nm  |
|  :::  |  :::  |  :::  |  545-562 nm  |  577-602 nm  |
|  :::  |  :::  |  :::  |  620-642 nm  |  658-704 nm  |
|  6  |  none  |  |  |  |

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==== Emission Filtersets (Confocal) ====

{{:zeiss_cell_observer_sd_filters_confocal.jpg  |}}

^  Position  ^  Transmission  ^
|  1: BP 450/50  |  425-475 nm  |
|  2: TBP 526 601 688  |  510-542 nm  |
|  :::  |  586-616 nm  |  
|  :::  |  663-713 nm  |
|  3: none  |  |
|  4: FE01-520/35  |  502-537 nm  |
|  5: BP 600 50  |  575-625 nm  |
|  6: BP 690/50  |  665-715 nm  |

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