zeiss_cell_observer_sd
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zeiss_cell_observer_sd [2020/07/21 23:23] bioimaging |
zeiss_cell_observer_sd [2020/10/27 18:26] bioimaging |
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| <b>Model</b>: Cell Observer SD<br> | <b>Model</b>: Cell Observer SD<br> | ||
| <b>Nickname</b>: "Cell Observer SD"<br> | <b>Nickname</b>: "Cell Observer SD"<br> | ||
| - | <b>Software</b>: ZEN<br> | + | <b>Software</b>: ZEN 2.6 (blue edition)<br> |
| <b>Year</b>: 2015 <br> | <b>Year</b>: 2015 <br> | ||
| <b>SN</b>: 3851001637 <br> | <b>SN</b>: 3851001637 <br> | ||
| Line 72: | Line 72: | ||
| | 10x | EC Plan-Neofluar | Air | 0.30 | 5.20 | [[https://www.micro-shop.zeiss.com/en/de/shop/objectives/420340-9901-000/Objective-EC-Plan-Neofluar-10x-0.30-M27|420340-9901-000]] | | | 10x | EC Plan-Neofluar | Air | 0.30 | 5.20 | [[https://www.micro-shop.zeiss.com/en/de/shop/objectives/420340-9901-000/Objective-EC-Plan-Neofluar-10x-0.30-M27|420340-9901-000]] | | ||
| | 25x | LCI Plan-Neofluar Corr DIC | Oil/Glyc/W | 0.80 | 0.21 | [[https://www.micro-shop.zeiss.com/en/de/shop/objectives/420852-9972-000/Objective-LCI-Plan-Neofluar-25x-0.8-Imm-Corr-DIC-M27|420852-9972-000]] | | | 25x | LCI Plan-Neofluar Corr DIC | Oil/Glyc/W | 0.80 | 0.21 | [[https://www.micro-shop.zeiss.com/en/de/shop/objectives/420852-9972-000/Objective-LCI-Plan-Neofluar-25x-0.8-Imm-Corr-DIC-M27|420852-9972-000]] | | ||
| - | | 63x | Plan-Apochromat Ph3 | Oil | 1.40 | 0.19 | [[https://www.micro-shop.zeiss.com/en/de/shop/objectives/420781-9910-000/Objective-Plan-Apochromat-63-1.4-Oil-Ph3-M27|420781-9910-000]] | | ||
| ==== Filtersets (Widefield) ==== | ==== Filtersets (Widefield) ==== | ||
| Line 86: | Line 85: | ||
| | ::: | ::: | ::: | 620-642 nm | 650 nm | 658-704 nm | | | ::: | ::: | ::: | 620-642 nm | 650 nm | 658-704 nm | | ||
| | 6 | Empty ||||| | | 6 | Empty ||||| | ||
| + | |||
| ==== Emission Filters (Confocal) ==== | ==== Emission Filters (Confocal) ==== | ||
| Line 102: | Line 102: | ||
| | [[https://www.photometrics.com/wp-content/uploads/2019/10/Evolve512-Datasheet.pdf|Photometrics Evolve 512]] | EMCCD | 512 x 512 | 16 x 16 | > 90% | | | [[https://www.photometrics.com/wp-content/uploads/2019/10/Evolve512-Datasheet.pdf|Photometrics Evolve 512]] | EMCCD | 512 x 512 | 16 x 16 | > 90% | | ||
| | [[https://www.hamamatsu.com/resources/pdf/sys/SCAS0081E_C11440-22CU.pdf|Hamamatsu ORCA-flash4.0 V2]] | sCMOS | 2048 x 2048 | 6.5 x 6.5 | 82% | | | [[https://www.hamamatsu.com/resources/pdf/sys/SCAS0081E_C11440-22CU.pdf|Hamamatsu ORCA-flash4.0 V2]] | sCMOS | 2048 x 2048 | 6.5 x 6.5 | 82% | | ||
| + | |||
| + | ===== Microscope Turn On Procedure ===== | ||
| + | * Turn on the computer | ||
| + | * Login in to Windows (Bioimaging User) | ||
| + | * Turn on the black socket outlets underneath the microscope table by order (1→2→3) | ||
| + | * Wait 2 min to allow for system components detection | ||
| + | * Start the **ZEN Blue** software | ||
| + | |||
| + | **Warning**: If you need to change the stage adapter, please contact the <html><a href="/facility/bioimaging/doku.php?id=about">Bioimaging Unit</a> (<b>imm-bioimaging@medicina.ulisboa.pt</b> | <img src="/facility/bioimaging/lib/exe/fetch.php?media=phone_neg.png" width=15> 47222</html>) | ||
| + | |||
| + | ===== Microscope Turn Off Procedure ===== | ||
| + | If there is another user for this microscope in the __next hour__: | ||
| + | * Exit the **ZEN** software, leave the lasers on | ||
| + | * Log off the computer | ||
| + | * Clean up immersion objectives | ||
| + | Else: | ||
| + | * Exit the **ZEN** software | ||
| + | * Shut down the computer | ||
| + | * Turn off the black socket outlets underneath the microscope table by reverse order (3→2→1) | ||
| ---- | ---- | ||
| [[resources|Back to the Resources page]] | [[resources|Back to the Resources page]] | ||
zeiss_cell_observer_sd.txt · Last modified: 2024/04/01 23:53 by bioimaging
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