zeiss_cell_observer_sd
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| Both sides previous revision Previous revision Next revision | Previous revision Next revision Both sides next revision | ||
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zeiss_cell_observer_sd [2020/02/28 00:39] bioimaging [System overview] |
zeiss_cell_observer_sd [2020/07/21 16:15] bioimaging |
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|   <br> |   <br> | ||
| - | → <a href="https://imm.medicina.ulisboa.pt/intranet/booking/119"><img src="/facility/bioimaging/lib/exe/fetch.php?media=date.png"> Zeiss Cell Observer SD Booking</a> | + | → <a href="https://my.agendo.science/calendar/?id=MjVjYWxlbmRhcnM="><img src="/facility/bioimaging/lib/exe/fetch.php?media=date.png"> Zeiss Cell Observer SD Booking</a> |
| <br> | <br> | ||
| → <a href="/facility/bioimaging/doku.php?id=zeiss_cell_observer_sd_quality"><img src="/facility/bioimaging/lib/exe/fetch.php?media=list-icon.png" width=18> Zeiss Cell Observer SD Quality Control</a> | → <a href="/facility/bioimaging/doku.php?id=zeiss_cell_observer_sd_quality"><img src="/facility/bioimaging/lib/exe/fetch.php?media=list-icon.png" width=18> Zeiss Cell Observer SD Quality Control</a> | ||
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| * **sCMOS Camera**: [[https://www.hamamatsu.com/resources/pdf/sys/SCAS0081E_C11440-22CU.pdf|Hamamatsu ORCA-flash4.0 V2]] | * **sCMOS Camera**: [[https://www.hamamatsu.com/resources/pdf/sys/SCAS0081E_C11440-22CU.pdf|Hamamatsu ORCA-flash4.0 V2]] | ||
| * [[https://www.zeiss.com/microscopy/int/products/light-microscopes/axio-observer-for-biology/definite-focus.html|Definite Focus.2]] | * [[https://www.zeiss.com/microscopy/int/products/light-microscopes/axio-observer-for-biology/definite-focus.html|Definite Focus.2]] | ||
| + | * | [[https://www.youtube.com/watch?v=Che0bouWcVE|Introduction to Definite Focus.2]] | ||
| + | * | [[https://www.youtube.com/watch?v=I_c3SAHF984|Perform Large Tile Experiments with Definite Focus.2]] | ||
| + | * | [[https://www.youtube.com/watch?v=IUvdvSNsE1I|Multi-Position Experiments with Definite Focus.2]] | ||
| + | * | [[https://www.youtube.com/watch?v=DqqV6cs3mZg|Multi-Position Time Series with Definite Focus.2]] | ||
| {{:warning.png?25|}} Data files older than **1 month** will be automatically deleted on this system, please copy your data to the iMM server using the desktop link. | {{:warning.png?25|}} Data files older than **1 month** will be automatically deleted on this system, please copy your data to the iMM server using the desktop link. | ||
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| ^ Laser Unit ^ Wavelength ^ Maximum Power ^ Current Status ^ | ^ Laser Unit ^ Wavelength ^ Maximum Power ^ Current Status ^ | ||
| | Solid State Laser 405 | 405 nm | 50 mW | ok | | | Solid State Laser 405 | 405 nm | 50 mW | ok | | ||
| - | | Solid State Laser 488 | 488 nm | 100 mW | **not working** | | + | | Solid State Laser 488 | 488 nm | 100 mW | ok | |
| | Solid State Laser 561 | 561 nm | 75 mW | ok | | | Solid State Laser 561 | 561 nm | 75 mW | ok | | ||
| | Solid State Laser 638 | 638 nm | 75 mW | ok | | | Solid State Laser 638 | 638 nm | 75 mW | ok | | ||
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| ==== Filtersets (Widefield) ==== | ==== Filtersets (Widefield) ==== | ||
| - | ^ Position ^ Filterset ^ Reference ^ Excitation ^ Emission ^ | + | ^ Position ^ Filterset ^ Reference ^ Excitation ^ Dichroic ^ Emission ^ |
| - | | 1 | Green | [[https://www.micro-shop.zeiss.com/?s=7139069fdc79c&l=en&p=us&f=f&a=v&b=f&id=489038-9901-000&o=|FS38HE]] | 450-490 nm | 500-550 nm | | + | | 1 | Green | [[https://www.micro-shop.zeiss.com/?s=7139069fdc79c&l=en&p=us&f=f&a=v&b=f&id=489038-9901-000&o=|FS38HE]] | 450-490 nm | 495 nm | 500-550 nm | |
| - | | 2 | Red | [[https://www.micro-shop.zeiss.com/?s=4237481102cf7b&l=en&p=us&f=f&a=v&b=f&id=489043-9901-000&o=|FS43HE]] | 538-562 nm | 570-640 nm | | + | | 2 | Red | [[https://www.micro-shop.zeiss.com/?s=4237481102cf7b&l=en&p=us&f=f&a=v&b=f&id=489043-9901-000&o=|FS43HE]] | 538-562 nm | 570 nm | 570-640 nm | |
| - | | 3 | Blue | [[https://www.micro-shop.zeiss.com/?s=163176628fcf844&l=en&p=us&f=f&a=v&b=f&id=488049-9901-000&o=|FS49]] | 335-383 nm | 420-470 nm | | + | | 3 | Blue | [[https://www.micro-shop.zeiss.com/?s=163176628fcf844&l=en&p=us&f=f&a=v&b=f&id=488049-9901-000&o=|FS49]] | 335-383 nm | 395 nm | 420-470 nm | |
| - | | 4 | Far Red | [[https://www.micro-shop.zeiss.com/?s=4237481102cf7b&l=en&p=us&f=f&a=v&b=f&id=488050-9901-000&o=|FS50]] | 625-655 nm | 665-715 nm | | + | | 4 | Far Red | [[https://www.micro-shop.zeiss.com/?s=4237481102cf7b&l=en&p=us&f=f&a=v&b=f&id=488050-9901-000&o=|FS50]] | 625-655 nm | 660 nm | 665-715 nm | |
| - | | 5 | QUAD | [[https://www.micro-shop.zeiss.com/?s=4237481102cf7b&l=en&p=us&f=f&a=v&b=f&id=489081-9010-000&o=| FS81HE]] | 357-393 nm | 407-412 nm | | + | | 5 | QUAD | [[https://www.micro-shop.zeiss.com/?s=4237481102cf7b&l=en&p=us&f=f&a=v&b=f&id=489081-9010-000&o=| FS81HE]] | 357-393 nm | 400 nm | 407-412 nm | |
| - | | ::: | ::: | ::: | 473-496 nm | 511-528 nm | | + | | ::: | ::: | ::: | 473-496 nm | 503 nm | 511-528 nm | |
| - | | ::: | ::: | ::: | 545-562 nm | 577-602 nm | | + | | ::: | ::: | ::: | 545-562 nm | 570 nm | 577-602 nm | |
| - | | ::: | ::: | ::: | 620-642 nm | 658-704 nm | | + | | ::: | ::: | ::: | 620-642 nm | 650 nm | 658-704 nm | |
| - | | 6 | none | | | | | + | | 6 | Empty ||||| |
| ==== Emission Filters (Confocal) ==== | ==== Emission Filters (Confocal) ==== | ||
zeiss_cell_observer_sd.txt · Last modified: 2024/04/01 23:53 by bioimaging
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