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zeiss_cell_observer_sd [2018/06/07 10:59]
bioimaging
zeiss_cell_observer_sd [2020/01/01 17:29]
bioimaging
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-<img src="/​facility/​bioimaging/​lib/​exe/​fetch.php?​media=zeiss_cell_observer_sd_name.jpg">+<img src="/​facility/​bioimaging/​lib/​exe/​fetch.php?​media=zeiss_cell_observer_sd_name_new.png">
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-<a href="/​facility/​bioimaging/​lib/​exe/​fetch.php?​media=zeiss_cell_observer_sd_wiki.jpg"><​img src="/​facility/​bioimaging/​lib/​exe/​fetch.php?​media=zeiss_cell_observer_sd_wiki.jpg" width=300></​a>​+<a href="/​facility/​bioimaging/​lib/​exe/​fetch.php?​media=zeiss_cell_observer_sd_wiki_new.png"><​img src="/​facility/​bioimaging/​lib/​exe/​fetch.php?​media=zeiss_cell_observer_sd_wiki_new.png" width=300></​a>​
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-<​b>​Location</​b>:​ Room P2-A-22 (<img src="/​facility/​bioimaging/​lib/​exe/​fetch.php?​media=telephone_icon.gif" width=15>​ 47217) <​br> ​+<​b>​Location</​b>: ​<a href="/​facility/​bioimaging/​lib/​exe/​fetch.php?​media=zeiss_cell_observer_sd_map_new.png">​Room P2-A-22</​a> ​(<img src="/​facility/​bioimaging/​lib/​exe/​fetch.php?​media=phone_neg.png" width=15>​ 47217) <​br> ​ 
 <​b>​Manufacturer</​b>:​ <a href="​http://​www.zeiss.de/​micro"​ target="​_blank">​Carl Zeiss Microimaging</​a><​br>​ <​b>​Manufacturer</​b>:​ <a href="​http://​www.zeiss.de/​micro"​ target="​_blank">​Carl Zeiss Microimaging</​a><​br>​
-<​b>​Model</​b>: ​<a href="​http://​www.zeiss.com/​microscopy/​en_de/​products/​confocal-microscopes/​cell-observer-sd-for-biology.html"​ target="​_blank">​Cell Observer SD</a><br>+<​b>​Model</​b>:​ Cell Observer SD<​br>​
 <​b>​Nickname</​b>:​ "Cell Observer SD"<​br>​ <​b>​Nickname</​b>:​ "Cell Observer SD"<​br>​
 <​b>​Software</​b>:​ ZEN<​br>​ <​b>​Software</​b>:​ ZEN<​br>​
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 ===== System overview ===== ===== System overview =====
  
-{{  :​spinning_disk.jpg?0x300|}} The Zeiss Cell Observer SD //spinning disk confocal microscope//​ is a fast imaging system which provides a trade-off between confocality,​ resolution and speed. It is an inverted microscope ideal for live cell applications which require fast acquisition speeds rather than high resolution images. The scanning unit achieves confocality by directing light through a spinning disk with many small pinholes. Images are then acquired with a sensitive EMCCD which allows for very small exposure times but is limited in resolution to 512x512 pixels. The stage is motorized and furthermore equipped with a piezo for Z displacement so fast 4D imaging is possible in multiple stage positions. The system is also equipped with a large size incubator for temperature control, a small stage incubator for CO2 supply and with Definite Focus for hardware focus control during long time-lapse acquisitions. You can also use the system as a widefield microscope, using a sCMOS camera for acquisition. Even though its resolution is not as high as a //​point-scanning confocal system// such as the [[zeiss_lsm_880|Zeiss LSM 880]] or [[zeiss_lsm_710|Zeiss LSM 710]], it is much faster than these two systems. You can also check the [[3i_marianas_sdc|3i Marianas SDC]] if you need a spinning disk with higher laser power illumination. If your personal computer cannot handle all the data you collected, check out the [[big_guy|Big Guy]] or [[colossus|Colossus]].+{{  :​spinning_disk.png|}} The Zeiss Cell Observer SD //spinning disk confocal microscope//​ is a fast imaging system which provides a trade-off between confocality,​ resolution and speed. It is an inverted microscope ideal for live cell applications which require fast acquisition speeds rather than high resolution images. The scanning unit achieves confocality by directing light through a spinning disk with many small pinholes. Images are then acquired with a sensitive EMCCD which allows for very small exposure times but is limited in resolution to 512x512 pixels. The stage is motorized and furthermore equipped with a piezo for Z displacement so fast 4D imaging is possible in multiple stage positions. The system is also equipped with a large size incubator for temperature control, a small stage incubator for CO2 supply and with Definite Focus for hardware focus control during long time-lapse acquisitions. You can also use the system as a widefield microscope, using a sCMOS camera for acquisition. Even though its resolution is not as high as a //​point-scanning confocal system// such as the [[zeiss_lsm_880|Zeiss LSM 880]] or [[zeiss_lsm_710|Zeiss LSM 710]], it is much faster than these two systems. You can also check the [[3i_marianas_sdc|3i Marianas SDC]] if you need a spinning disk with higher laser power illumination. If your personal computer cannot handle all the data you collected, check out the [[big_guy|Big Guy]] or [[colossus|Colossus]].
  
   * **Microscope**:​ [[http://​www.zeiss.com/​microscopy/​en_de/​products/​light-microscopes/​axio-observer-for-biology.html|Zeiss Axio Observer]]   * **Microscope**:​ [[http://​www.zeiss.com/​microscopy/​en_de/​products/​light-microscopes/​axio-observer-for-biology.html|Zeiss Axio Observer]]
-  * **Confocal scanner**: [[http://www.andor.com/microscopy_systems/peripherals/confocal_scanners/|Yokogawa CSU-x1]] +  * **Confocal scanner**: [[https://www.yokogawa.com/eu/solutions/products-platforms/​life-science/​spinning-disk-confocal/​csu-x1-confocal-scanner-unit/|Yokogawa CSU-x1]] 
-  * **EMCCD Camera**: [[http://​www.photometrics.com/​products/emccdcams/evolve/512.php|Evolve 512 EMCCD]] +  * **EMCCD Camera**: [[https://​www.photometrics.com/​wp-content/uploads/2019/10/​Evolve512-Datasheet.pdf|Evolve 512 EMCCD]] 
-  * **sCMOS Camera**: [[http://​www.hamamatsu.com/​eu/en/community/life_science_camera/​product/​search/​C11440-22CU/index.html|Hamamatsu ORCA-flash4.0 V2]]+  * **sCMOS Camera**: [[https://​www.hamamatsu.com/​resources/pdf/sys/SCAS0081E_C11440-22CU.pdf|Hamamatsu ORCA-flash4.0 V2]] 
 +  * [[https://​www.zeiss.com/​microscopy/​int/​products/​light-microscopes/​axio-observer-for-biology/​definite-focus.html|Definite Focus.2]]
  
-{{warning.jpg?20|}} Data files older than **1 month** will be automatically deleted on this system, please copy your data to the iMM server using the desktop link.+{{:warning.png?25|}} Data files older than **1 month** will be automatically deleted on this system, please copy your data to the iMM server using the desktop link.
  
 ===== System components ===== ===== System components =====
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 ==== Objectives ==== ==== Objectives ====
  
-{{:​zeiss_cell_observer_sd_objectives.jpg ​ |}} +^  Magnification ​  ​^ ​ Model  ^  ​Immersion ​ ​^ ​ NA  ^  WD (mm)  ​^ ​ Reference ​ ^ 
-^  Magnification ​  ​^ ​ Model  ^  ​Type  ​^ ​ NA  ^  WD (mm)  ^ +|  20x  ​| ​ Plan-Apochromat Ph2  |  Air  |  0.8  |  0.55  ​| ​ [[https://​www.micro-shop.zeiss.com/​en/​de/​shop/​objectives/​420651-9911-000/Objective-Plan-Apochromat-20x-0.8-Ph2-M27|420651-9911-000]]  ​
-|  20x  |  [[https://​www.micro-shop.zeiss.com/​?​s=42373720ae8af6&​l=en&​p=us&​f=o&​a=v&​m=s&​id=420651-9911-000|Plan-Apochromat]] ​ |  ​Dry  ​|  ​0.8  ​|  ​0.55  +|  ​40x  ​|  ​LD C-Apochromat Corr  ​|  ​Water  |  1.10  ​|  ​0.62  ​| ​ [[https://​www.micro-shop.zeiss.com/​en/​de/​shop/​objectives/​421867-9970-000/Objective-LD-C-Apochromat-40x-1.1-W-Corr-M27|421867-9970-000]]  ​
-|  ​40x  ​| ​ [[https://​www.micro-shop.zeiss.com/​?​s=42373720ae8af6&​l=en&​p=us&​f=o&​a=v&​m=s&​id=421867-9970-000|LD C-Apochromat]] ​ |  ​Water  ​| ​ 1.10  ​| ​ 0.62  | +|  ​40x  |  Plan-Apochromat DIC  |  Oil  ​| ​ 1.40  ​| ​ 0.13  ​| ​ [[https://​www.micro-shop.zeiss.com/​en/​de/​shop/​objectives/​420762-9900-000/Objective-Plan-Apochromat-40x-1.4-Oil-DIC-M27|420762-9900-000]]  | 
-|  40x  ​| ​ [[https://​www.micro-shop.zeiss.com/​?​s=42373720ae8af6&​l=en&​p=us&​f=o&​a=v&​m=s&​id=420762-9900-000|Plan-Apochromat]] ​ |  Oil  |  1.40  |  0.13  | +|  63x  |  Plan-Apochromat DIC  ​| ​ Oil  |  1.40  |  0.19  ​| ​ [[https://​www.micro-shop.zeiss.com/​en/​de/​shop/​objectives/​420782-9900-000/Objective-Plan-Apochromat-63x-1.4-Oil-DIC-M27|420782-9900-000]]  | 
-|  63x  ​| ​ [[https://​www.micro-shop.zeiss.com/​?​s=3407786617d462&​l=en&​p=uk&​f=o&​a=v&​m=s&​id=420782-9900-000|Plan-Apochromat]] ​ |  Oil  |  1.40  |  0.19  | +|  63x  |  Plan-Apochromat Ph3  ​| ​ Oil  |  1.40  |  0.19  |  [[https://​www.micro-shop.zeiss.com/​en/​de/​shop/​objectives/​420781-9910-000/Objective-Plan-Apochromat-63-1.4-Oil-Ph3-M27|420781-9910-000]]  | 
-|  63x  ​| ​ [[https://​www.micro-shop.zeiss.com/​?​s=4238899732b41f&​l=en&​p=us&​f=o&​a=v&​m=s&​id=420781-9910-000|Plan-Apochromat Ph3]]  |  Oil  |  1.40  ​| ​ 0.19  | +|  100x  |  α Plan-Apochromat DIC  ​| ​ Oil  |  1.46  ​| ​ 0.11  ​| ​ [[https://​www.micro-shop.zeiss.com/​en/​de/​shop/​objectives/​420792-9800-000/Objective-alpha-Plan-Apochromat-100x-1.46-Oil-DIC-M27|420792-9800-000]] ​ |
-|  100x  ​| ​ [[https://​www.micro-shop.zeiss.com/​?​s=7138707e1a1de&​l=en&​p=uk&​f=o&​a=v&​m=s&​id=420792-9800-000|alpha Plan-Apochromat]]  |  Oil  |  ​1.46   ​0.11 ​ | +
- +
-<​html>​ +
-<​br>​ +
-<​br>​ +
-<​br>​ +
-<​br>​ +
-</​html>​+
  
 ==== Filtersets (Widefield) ==== ==== Filtersets (Widefield) ====
- 
-{{:​zeiss_cell_observer_sd_filters_widefield.jpg ​ |}} 
  
 ^  Position ​ ^  Filterset ​ ^  Reference ​ ^  Excitation ​ ^  Emission ​ ^ ^  Position ​ ^  Filterset ​ ^  Reference ​ ^  Excitation ​ ^  Emission ​ ^
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 |  :::  |  :::  |  :::  |  620-642 nm  |  658-704 nm  | |  :::  |  :::  |  :::  |  620-642 nm  |  658-704 nm  |
 |  6  |  none  |  |  |  | |  6  |  none  |  |  |  |
- 
-<​html>​ 
-<br> 
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- 
 ==== Emission Filtersets (Confocal) ==== ==== Emission Filtersets (Confocal) ====
- 
-{{:​zeiss_cell_observer_sd_filters_confocal.jpg ​ |}} 
  
 ^  Position ​ ^  Transmission ​ ^ ^  Position ​ ^  Transmission ​ ^
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 |  5: BP 600 50  |  575-625 nm  | |  5: BP 600 50  |  575-625 nm  |
 |  6: BP 690/​50 ​ |  665-715 nm  | |  6: BP 690/​50 ​ |  665-715 nm  |
 +
 +==== Cameras ====
 +^  Model  ^  Type  ^  Frame Size  ^  Pixel Size (µm)  ^  Quantum Efficiency ​ ^
 +|  [[https://​www.photometrics.com/​wp-content/​uploads/​2019/​10/​Evolve512-Datasheet.pdf|Photometrics Evolve 512]]  |  EMCCD  |  512 x 512  |  16 x 16  |  > 90%  |
 +|  [[https://​www.hamamatsu.com/​resources/​pdf/​sys/​SCAS0081E_C11440-22CU.pdf|Hamamatsu ORCA-flash4.0 V2]]  |  sCMOS  |  2048 x 2048  |  6.5 x 6.5  |  82%  |
  
 ---- ----
  
 [[resources|Back to the Resources page]] [[resources|Back to the Resources page]]
zeiss_cell_observer_sd.txt · Last modified: 2020/02/28 00:39 by bioimaging