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zeiss_cell_observer [2020/01/14 18:40]
bioimaging [Microscope Turn Off procedures]
zeiss_cell_observer [2020/07/21 23:26] (current)
bioimaging
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 &nbsp <br> &nbsp <br>
 &nbsp <br> &nbsp <br>
-&rarr; <a href="​https://​imm.medicina.ulisboa.pt/​intranet/booking/125"><​img src="/​facility/​bioimaging/​lib/​exe/​fetch.php?​media=date.png"> ​ Zeiss Cell Observer Booking</​a>​+&rarr; <a href="​https://​my.agendo.science/calendar/?​id=MjZjYWxlbmRhcnM="><​img src="/​facility/​bioimaging/​lib/​exe/​fetch.php?​media=date.png"> ​ Zeiss Cell Observer Booking</​a>​
  <​br> ​  <​br> ​
 &rarr; <a href="/​facility/​bioimaging/​doku.php?​id=zeiss_cell_observer_quality"><​img src="/​facility/​bioimaging/​lib/​exe/​fetch.php?​media=list-icon.png"​ width=18>​ Zeiss Cell Observer Quality Control</​a>​ &rarr; <a href="/​facility/​bioimaging/​doku.php?​id=zeiss_cell_observer_quality"><​img src="/​facility/​bioimaging/​lib/​exe/​fetch.php?​media=list-icon.png"​ width=18>​ Zeiss Cell Observer Quality Control</​a>​
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 ===== Microscope overview ===== ===== Microscope overview =====
-The Zeiss Cell Observer is a fully motorized inverted //widefield fluorescence microscope//​ ideal for live-cell imaging applications. It is equipped with a large cage incubator and a small stage incubator for temperature control and CO2 supply and with Definite Focus for hardware focus control during long time-lapse acquisitions. Using the ZEN software you can control the motorized filters, shutters and stage to simultaneously set up multi-color time-lapse imaging experiments in multiple stage positions. Its sensitive cooled Axiocam 506m camera allows you to capture weak fluorescent signals and minimize photobleaching/​photodamage in light sensitive samples. You can also perform Z-stack acquisition and use [[https://​huygens.i3s.up.pt/​login.php|PPBI Huygens RM]] [[http://​micro.magnet.fsu.edu/​primer/​digitalimaging/​deconvolution/​deconvolutionhome.html|deconvolution]] server to significantly improve image resolution even in thick samples. If don't want to use deconvolution,​ check out one of the confocal systems such as the point scanners [[zeiss_lsm_710|Zeiss LSM 710]] and [[zeiss_lsm_880|Zeiss LSM 880]] or spinning disks [[3i_marianas_sdc|3i Marianas SDC]] and [[zeiss_cell_observer_sd|Zeiss Cell Observer SD]]. If your personal computer cannot handle all the data you collected, check out the [[big_guy|Big Guy]] or [[colossus|Colossus]]. If you have FISH or other fixed fluorescence slides try the [[leica_dm5000b|Leica DM5000B]] instead.+{{  :​widefield_lightpath.png?​0x300}}The Zeiss Cell Observer is a fully motorized inverted //widefield fluorescence microscope//​ ideal for live-cell imaging applications. It is equipped with a large cage incubator and a small stage incubator for temperature control and CO2 supply and with Definite Focus for hardware focus control during long time-lapse acquisitions. Using the ZEN software you can control the motorized filters, shutters and stage to simultaneously set up multi-color time-lapse imaging experiments in multiple stage positions. Its sensitive cooled Axiocam 506m camera allows you to capture weak fluorescent signals and minimize photobleaching/​photodamage in light sensitive samples. You can also perform Z-stack acquisition and use [[https://​huygens.i3s.up.pt/​login.php|PPBI Huygens RM]] [[http://​micro.magnet.fsu.edu/​primer/​digitalimaging/​deconvolution/​deconvolutionhome.html|deconvolution]] server to significantly improve image resolution even in thick samples. If don't want to use deconvolution,​ check out one of the confocal systems such as the point scanners [[zeiss_lsm_710|Zeiss LSM 710]] and [[zeiss_lsm_880|Zeiss LSM 880]] or spinning disks [[3i_marianas_sdc|3i Marianas SDC]] and [[zeiss_cell_observer_sd|Zeiss Cell Observer SD]]. If your personal computer cannot handle all the data you collected, check out the [[big_guy|Big Guy]] or [[colossus|Colossus]].
  
   * **Microscope**:​ [[http://​www.zeiss.com/​microscopy/​en_de/​products/​light-microscopes/​axio-observer-for-biology.html|Zeiss Axio Observer]]   * **Microscope**:​ [[http://​www.zeiss.com/​microscopy/​en_de/​products/​light-microscopes/​axio-observer-for-biology.html|Zeiss Axio Observer]]
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 ===== Booking Rules ===== ===== Booking Rules =====
-The Zeiss Cell Observer booking is divided in three daily slots: +  ​- **Users** can book at maximum **8 hours per week** 
-|  9:00 to 13:00  |  13:00 to 17:00  |  17:00 to 21:00  | +  - This usage restriction does not apply for **weekends** and for working ​days **before 9:00** and **after 21:00**
- +
-  ​**user** can book at maximum **2 sessions ​per week** +
-  - Each **session must fit inside one of the three daily slots** (i.e. a session booked from 11:00 to 14:00 counts as 2 sessions) +
-  - The above rules do not apply for **weekends** and for work days **before 9:00** and **after 21:00** +
-  - The above rules do not apply for sessions booked on the very same day or **24 hours ahead**+
   - Exceptions to these rules require **approval from [[joserino@medicina.ulisboa.pt|José Rino]]**.   - Exceptions to these rules require **approval from [[joserino@medicina.ulisboa.pt|José Rino]]**.
-  - Users must indicate if they will use the incubator in the booking Description field (write down 37C INCUBATOR) 
  
 ===== System components ===== ===== System components =====
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 ==== Filtersets ==== ==== Filtersets ====
  
-^  Position ​ ^  Filterset ​ ^  Reference ​ ^  Excitation ​ ^  Emission ​ ^ +^  Position ​ ^  Filterset ​ ^  Reference ​ ^  Excitation ​ ​^ ​ Dichroic ​ ​^ ​ Emission ​ ^ 
-|  1  |  HE GFP  |  [[https://​www.micro-shop.zeiss.com/?​s=23108437692794b&​l=en&​p=us&​f=f&​a=v&​b=f&​id=489038-9901-000&​o=|FS38HE]] ​ |  450-490 nm  |  500-550 nm  | +|  1  |  HE GFP  |  [[https://​www.micro-shop.zeiss.com/?​s=23108437692794b&​l=en&​p=us&​f=f&​a=v&​b=f&​id=489038-9901-000&​o=|FS38HE]] ​ |  450-490 ​nm  |  495 nm  |  500-550 nm  | 
-|  2  |  HE DsRed  |  [[https://​www.micro-shop.zeiss.com/?​s=23108437692794b&​l=en&​p=us&​f=f&​a=v&​b=f&​id=489043-9901-000&​o=|FS43HE]] ​ |  538-562 nm  |  570-640 nm  | +|  2  |  HE DsRed  |  [[https://​www.micro-shop.zeiss.com/?​s=23108437692794b&​l=en&​p=us&​f=f&​a=v&​b=f&​id=489043-9901-000&​o=|FS43HE]] ​ |  538-562 ​nm  |  570 nm  |  570-640 nm  | 
-|  3  |  DAPI  |  [[https://​www.micro-shop.zeiss.com/?​s=23108437692794b&​l=en&​p=us&​f=f&​a=v&​b=f&​id=488049-9901-000&​o=|FS49]] ​ |  335-383 nm  |  420-470 nm  | +|  3  |  DAPI  |  [[https://​www.micro-shop.zeiss.com/?​s=23108437692794b&​l=en&​p=us&​f=f&​a=v&​b=f&​id=488049-9901-000&​o=|FS49]] ​ |  335-383 ​nm  |  395 nm  |  420-470 nm  | 
-|  4  |  Cy5  |  [[https://​www.micro-shop.zeiss.com/?​s=23108437692794b&​l=en&​p=us&​f=f&​a=v&​b=f&​id=488050-9901-000&​o=|FS50]] ​ |  625-655 nm  |  665-715 nm  | +|  4  |  Cy5  |  [[https://​www.micro-shop.zeiss.com/?​s=23108437692794b&​l=en&​p=us&​f=f&​a=v&​b=f&​id=488050-9901-000&​o=|FS50]] ​ |  625-655 ​nm  |  660 nm  |  665-715 nm  | 
-|  5  |  ​none  ​| ​ ​none  ​ ​none  ​ ​none  ​+|  5  |  ​Empty  ||||
-|  6  |  ​none  ​| ​ ​none  ​ ​none  ​ ​none  ​|+|  6  |  ​Empty  |||||
  
 ==== Objectives ==== ==== Objectives ====
 ^  Magnification ​ ^  Model  ^  Immersion ​ ^  NA  ^  WD (mm)  ^  Reference ​ ^ ^  Magnification ​ ^  Model  ^  Immersion ​ ^  NA  ^  WD (mm)  ^  Reference ​ ^
-|  10x  |  EC Plan-Neofluar ​ |  Air  |  0.30  |  5. ​| ​ [[https://​www.micro-shop.zeiss.com/​en/​de/​shop/​objectives/​420340-9901-000/​Objective-EC-Plan-Neofluar-10x-0.30-M27|420340-9901-000]] ​ |+|  10x  |  EC Plan-Neofluar ​Ph1  ​| ​ Air  |  0.30  |  5.20  ​| ​ [[https://​www.micro-shop.zeiss.com/​en/​de/​shop/​objectives/​420341-9911-000/​Objective-EC-Plan-Neofluar-10x-0.30-Ph1-M27|420341-9911-000]] ​ | 
 +|  20x  |  EC Plan-Neofluar Ph 2  |  Air  |  0.50  |  2.00  |  [[https://​www.micro-shop.zeiss.com/​en/​de/​shop/​objectives/​440341-9904-000/​Objective-EC-Plan-Neofluar-20x-0.5-Ph2|440341-9904-000]] ​ |
 |  20x  |  Plan-Apochromat ​ |  Air  |  0.80  |  0.55  |  [[https://​www.micro-shop.zeiss.com/​en/​de/​shop/​objectives/​420650-9901-000/​Objective-Plan-Apochromat-20x-0.8-M27|420650-9901-000]] ​ | |  20x  |  Plan-Apochromat ​ |  Air  |  0.80  |  0.55  |  [[https://​www.micro-shop.zeiss.com/​en/​de/​shop/​objectives/​420650-9901-000/​Objective-Plan-Apochromat-20x-0.8-M27|420650-9901-000]] ​ |
-|  40x  |  EC Plan-NeoFluar ​ |  Air  |  0.75  |  0.71  |  [[https://​www.micro-shop.zeiss.com/​en/​de/​shop/​objectives/​440350-9903-000/​Objective-EC-Plan-Neofluar-40x-0.75|440350-9903-000]] ​ |+|  40x  |  EC Plan-NeoFluar ​ |  Air  |  0.75  |  0.71  |  [[https://​www.micro-shop.zeiss.com/​en/​de/​shop/​objectives/​420360-9900-000/​Objective-EC-Plan-Neofluar-40x-0.75-M27|420360-9900-000]] ​ |
 |  63x  |  Plan-Apochromat DIC  |  Oil  |  1.40  |  0.19  |  [[https://​www.micro-shop.zeiss.com/​en/​de/​shop/​objectives/​420782-9900-000/​Objective-Plan-Apochromat-63x-1.4-Oil-DIC-M27|420782-9900-000]] ​ | |  63x  |  Plan-Apochromat DIC  |  Oil  |  1.40  |  0.19  |  [[https://​www.micro-shop.zeiss.com/​en/​de/​shop/​objectives/​420782-9900-000/​Objective-Plan-Apochromat-63x-1.4-Oil-DIC-M27|420782-9900-000]] ​ |
 +|  63x  |  Plan-Apochromat Ph3  |  Oil  |  1.40  |  0.19  |  [[https://​www.micro-shop.zeiss.com/​en/​de/​shop/​objectives/​420781-9910-000/​Objective-Plan-Apochromat-63-1.4-Oil-Ph3-M27|420781-9910-000]] ​ |
  
 **Upon request:** **Upon request:**
 ^  Magnification ​ ^  Model  ^  Immersion ​ ^  NA  ^  WD (mm)  ^  Reference ​ ^ ^  Magnification ​ ^  Model  ^  Immersion ​ ^  NA  ^  WD (mm)  ^  Reference ​ ^
-|  1.25x  |  EC Epiplan-Neofluar ​ |  Air  |  0.035  |  4  |  [[https://​www.micro-shop.zeiss.com/​en/​de/​shop/​objectives/​422310-9900-000/​Objective-EC-Epiplan-Neofluar-1.25x-0.03-M27|422310-9900-000]] ​ | +|  1.25x  |  EC Epiplan-Neofluar ​ |  Air  |  0.035  |  4.00  ​| ​ [[https://​www.micro-shop.zeiss.com/​en/​de/​shop/​objectives/​422310-9900-000/​Objective-EC-Epiplan-Neofluar-1.25x-0.03-M27|422310-9900-000]] ​ | 
-|  5x  |  EC Plan-NeoFluar ​ |  Air  |  0.16  |  18. ​| ​ [[https://​www.micro-shop.zeiss.com/​en/​de/​shop/​objectives/​420330-9901-000/​Objective-EC-Plan-Neofluar-5x-0.16-M27|420330-9901-000]] ​ | +|  5x  |  EC Plan-NeoFluar ​ |  Air  |  0.16  |  18.50  ​| ​ [[https://​www.micro-shop.zeiss.com/​en/​de/​shop/​objectives/​420330-9901-000/​Objective-EC-Plan-Neofluar-5x-0.16-M27|420330-9901-000]] ​ | 
-|  ​20x  ​| ​ EC Plan-Neofluar ​Ph 2  ​| ​ Air  |  0.50  ​|  ​ ​| ​ [[https://​www.micro-shop.zeiss.com/​en/​de/​shop/​objectives/​440341-9904-000/​Objective-EC-Plan-Neofluar-20x-0.5-Ph2|440341-9904-000]] ​ |+|  ​10x  ​| ​ EC Plan-Neofluar ​ |  Air  |  0.30  ​|  ​5.20  ​| ​ [[https://​www.micro-shop.zeiss.com/​en/​de/​shop/​objectives/​420340-9901-000/​Objective-EC-Plan-Neofluar-10x-0.30-M27|420340-9901-000]] ​ |
 |  40x  |  Plan-Apochromat Corr  |  Air  |  0.95  |  0.25  |  [[https://​www.micro-shop.zeiss.com/​en/​de/​shop/​objectives/​420660-9970-000/​Objective-Plan-Apochromat-40x-0.95-Corr-M27|420660-9970-000]] ​ | |  40x  |  Plan-Apochromat Corr  |  Air  |  0.95  |  0.25  |  [[https://​www.micro-shop.zeiss.com/​en/​de/​shop/​objectives/​420660-9970-000/​Objective-Plan-Apochromat-40x-0.95-Corr-M27|420660-9970-000]] ​ |
 |  40x  |  Plan-Neofluar Ph3  |  Oil  |  1.30  |  0.20  |  [[https://​www.micro-shop.zeiss.com/​en/​de/​shop/​objectives/​440451-9903-000/​Objective-EC-Plan-Neofluar-40x-1.30-Oil-Ph3|440451-9903-000]] ​ | |  40x  |  Plan-Neofluar Ph3  |  Oil  |  1.30  |  0.20  |  [[https://​www.micro-shop.zeiss.com/​en/​de/​shop/​objectives/​440451-9903-000/​Objective-EC-Plan-Neofluar-40x-1.30-Oil-Ph3|440451-9903-000]] ​ |
-|  63x  |  Plan-Apochromat Ph3  |  Oil  |  1.4  |  0.19  |  [[https://​www.micro-shop.zeiss.com/​en/​de/​shop/​objectives/​420781-9910-000/​Objective-Plan-Apochromat-63-1.4-Oil-Ph3-M27|420781-9910-000]] ​ | 
 |  100x  |  Plan-Apochromat DIC  |  Oil  |  1.40  |  0.17  |  [[https://​www.micro-shop.zeiss.com/​en/​de/​shop/​objectives/​440782-9902-000/​Objective-Plan-Apochromat-100x-1.40-Oil-DIC|440782-9902-000]] ​ | |  100x  |  Plan-Apochromat DIC  |  Oil  |  1.40  |  0.17  |  [[https://​www.micro-shop.zeiss.com/​en/​de/​shop/​objectives/​440782-9902-000/​Objective-Plan-Apochromat-100x-1.40-Oil-DIC|440782-9902-000]] ​ |
  
zeiss_cell_observer.1579023638.txt.gz · Last modified: 2020/01/14 18:40 by bioimaging