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zeiss_cell_observer [2019/07/12 11:07]
bioimaging [Microscope Turn Off procedures]
zeiss_cell_observer [2020/07/09 03:41] (current)
bioimaging
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-<img src="/​facility/​bioimaging/​lib/​exe/​fetch.php?​media=zeiss_cell_observer_name.jpg">+<img src="/​facility/​bioimaging/​lib/​exe/​fetch.php?​media=zeiss_cell_observer_name_new.png">
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-<a href="/​facility/​bioimaging/​lib/​exe/​fetch.php?​media=zeiss_axio_observer_wiki.jpg"><​img src="/​facility/​bioimaging/​lib/​exe/​fetch.php?​media=zeiss_axio_observer_wiki.jpg" width=300></​a>​+<a href="/​facility/​bioimaging/​lib/​exe/​fetch.php?​media=zeiss_cell_observer_wiki_new.png"><​img src="/​facility/​bioimaging/​lib/​exe/​fetch.php?​media=zeiss_cell_observer_wiki_new.png" width=300></​a>​
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-<​b>​Location</​b>:​ <a href="/​facility/​bioimaging/​lib/​exe/​fetch.php?​media=zeiss_cell_observer_map.jpg">​Room P3-A-10</​a>​ (<img src="/​facility/​bioimaging/​lib/​exe/​fetch.php?​media=telephone_icon.gif" width=15>​ 47331) <​br> ​+<​b>​Location</​b>:​ <a href="/​facility/​bioimaging/​lib/​exe/​fetch.php?​media=zeiss_cell_observer_map_new.png">​Room P3-A-10</​a>​ (<img src="/​facility/​bioimaging/​lib/​exe/​fetch.php?​media=phone_neg.png" width=15>​ 47331) <​br> ​
 <​b>​Manufacturer</​b>:​ <a href="​http://​www.zeiss.de/​micro"​ target="​_blank">​Carl Zeiss MicroImaging</​a><​br>​ <​b>​Manufacturer</​b>:​ <a href="​http://​www.zeiss.de/​micro"​ target="​_blank">​Carl Zeiss MicroImaging</​a><​br>​
 <​b>​Model</​b>:​ Axio Observer<​br>​ <​b>​Model</​b>:​ Axio Observer<​br>​
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 &nbsp <br> &nbsp <br>
 &nbsp <br> &nbsp <br>
-&rarr; <a href="​https://​imm.medicina.ulisboa.pt/​intranet/booking/125"><​img src="/​facility/​bioimaging/​lib/​exe/​fetch.php?​media=date.png"> ​ Zeiss Cell Observer Booking</​a>​+&rarr; <a href="​https://​my.agendo.science/calendar/?​id=MjZjYWxlbmRhcnM="><​img src="/​facility/​bioimaging/​lib/​exe/​fetch.php?​media=date.png"> ​ Zeiss Cell Observer Booking</​a>​
  <​br> ​  <​br> ​
 &rarr; <a href="/​facility/​bioimaging/​doku.php?​id=zeiss_cell_observer_quality"><​img src="/​facility/​bioimaging/​lib/​exe/​fetch.php?​media=list-icon.png"​ width=18>​ Zeiss Cell Observer Quality Control</​a>​ &rarr; <a href="/​facility/​bioimaging/​doku.php?​id=zeiss_cell_observer_quality"><​img src="/​facility/​bioimaging/​lib/​exe/​fetch.php?​media=list-icon.png"​ width=18>​ Zeiss Cell Observer Quality Control</​a>​
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 ===== Microscope overview ===== ===== Microscope overview =====
-The Zeiss Cell Observer is a fully motorized inverted //widefield fluorescence microscope//​ ideal for live-cell imaging applications. It is equipped with a large cage incubator and a small stage incubator for temperature control and CO2 supply and with Definite Focus for hardware focus control during long time-lapse acquisitions. Using the ZEN software you can control the motorized filters, shutters and stage to simultaneously set up multi-color time-lapse imaging experiments in multiple stage positions. Its sensitive cooled Axiocam 506m camera allows you to capture weak fluorescent signals and minimize photobleaching/​photodamage in light sensitive samples. You can also perform Z-stack acquisition and use our [[http://​micro.magnet.fsu.edu/​primer/​digitalimaging/​deconvolution/​deconvolutionhome.html|deconvolution]] server ​[[brainstorm|Brainstorm]] ​to significantly improve image resolution even in thick samples. If don't want to use deconvolution,​ check out one of the confocal systems such as the point scanners [[zeiss_lsm_710|Zeiss LSM 710]] and [[zeiss_lsm_880|Zeiss LSM 880]] or spinning disks [[3i_marianas_sdc|3i Marianas SDC]] and [[zeiss_cell_observer_sd|Zeiss Cell Observer SD]]. If your personal computer cannot handle all the data you collected, check out the [[big_guy|Big Guy]] or [[colossus|Colossus]]. If you have FISH or other fixed fluorescence slides try the [[leica_dm5000b|Leica DM5000B]] instead.+{{  :​widefield_lightpath.png?​0x300}}The Zeiss Cell Observer is a fully motorized inverted //widefield fluorescence microscope//​ ideal for live-cell imaging applications. It is equipped with a large cage incubator and a small stage incubator for temperature control and CO2 supply and with Definite Focus for hardware focus control during long time-lapse acquisitions. Using the ZEN software you can control the motorized filters, shutters and stage to simultaneously set up multi-color time-lapse imaging experiments in multiple stage positions. Its sensitive cooled Axiocam 506m camera allows you to capture weak fluorescent signals and minimize photobleaching/​photodamage in light sensitive samples. You can also perform Z-stack acquisition and use [[https://​huygens.i3s.up.pt/​login.php|PPBI Huygens RM]] [[http://​micro.magnet.fsu.edu/​primer/​digitalimaging/​deconvolution/​deconvolutionhome.html|deconvolution]] server to significantly improve image resolution even in thick samples. If don't want to use deconvolution,​ check out one of the confocal systems such as the point scanners [[zeiss_lsm_710|Zeiss LSM 710]] and [[zeiss_lsm_880|Zeiss LSM 880]] or spinning disks [[3i_marianas_sdc|3i Marianas SDC]] and [[zeiss_cell_observer_sd|Zeiss Cell Observer SD]]. If your personal computer cannot handle all the data you collected, check out the [[big_guy|Big Guy]] or [[colossus|Colossus]].
  
   * **Microscope**:​ [[http://​www.zeiss.com/​microscopy/​en_de/​products/​light-microscopes/​axio-observer-for-biology.html|Zeiss Axio Observer]]   * **Microscope**:​ [[http://​www.zeiss.com/​microscopy/​en_de/​products/​light-microscopes/​axio-observer-for-biology.html|Zeiss Axio Observer]]
   * **Camera**: [[https://​www.zeiss.com/​microscopy/​int/​products/​microscope-cameras/​axiocam-506-mono.html|Axiocam 506 mono CCD]]   * **Camera**: [[https://​www.zeiss.com/​microscopy/​int/​products/​microscope-cameras/​axiocam-506-mono.html|Axiocam 506 mono CCD]]
  
-{{warning.jpg?20|}} Data files older than **1 month** will be automatically deleted on this system, please copy your data to the iMM server using the desktop link.+{{:warning.png?25|}} Data files older than **1 month** will be automatically deleted on this system, please copy your data to the iMM server using the desktop link.
  
 ===== Booking Rules ===== ===== Booking Rules =====
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 ==== Filtersets ==== ==== Filtersets ====
  
-^  Position ​ ^  Filterset ​ ^  Reference ​ ^  Excitation ​ ^  Emission ​ ^ +^  Position ​ ^  Filterset ​ ^  Reference ​ ^  Excitation ​ ​^ ​ Dichroic ​ ​^ ​ Emission ​ ^ 
-|  1  |  HE GFP  |  [[https://​www.micro-shop.zeiss.com/?​s=23108437692794b&​l=en&​p=us&​f=f&​a=v&​b=f&​id=489038-9901-000&​o=|FS38HE]] ​ |  450-490 nm  |  500-550 nm  | +|  1  |  HE GFP  |  [[https://​www.micro-shop.zeiss.com/?​s=23108437692794b&​l=en&​p=us&​f=f&​a=v&​b=f&​id=489038-9901-000&​o=|FS38HE]] ​ |  450-490 ​nm  |  495 nm  |  500-550 nm  | 
-|  2  |  HE DsRed  |  [[https://​www.micro-shop.zeiss.com/?​s=23108437692794b&​l=en&​p=us&​f=f&​a=v&​b=f&​id=489043-9901-000&​o=|FS43HE]] ​ |  538-562 nm  |  570-640 nm  | +|  2  |  HE DsRed  |  [[https://​www.micro-shop.zeiss.com/?​s=23108437692794b&​l=en&​p=us&​f=f&​a=v&​b=f&​id=489043-9901-000&​o=|FS43HE]] ​ |  538-562 ​nm  |  570 nm  |  570-640 nm  | 
-|  3  |  DAPI  |  [[https://​www.micro-shop.zeiss.com/?​s=23108437692794b&​l=en&​p=us&​f=f&​a=v&​b=f&​id=488049-9901-000&​o=|FS49]] ​ |  335-383 nm  |  420-470 nm  | +|  3  |  DAPI  |  [[https://​www.micro-shop.zeiss.com/?​s=23108437692794b&​l=en&​p=us&​f=f&​a=v&​b=f&​id=488049-9901-000&​o=|FS49]] ​ |  335-383 ​nm  |  395 nm  |  420-470 nm  | 
-|  4  |  Cy5  |  [[https://​www.micro-shop.zeiss.com/?​s=23108437692794b&​l=en&​p=us&​f=f&​a=v&​b=f&​id=488050-9901-000&​o=|FS50]] ​ |  625-655 nm  |  665-715 nm  | +|  4  |  Cy5  |  [[https://​www.micro-shop.zeiss.com/?​s=23108437692794b&​l=en&​p=us&​f=f&​a=v&​b=f&​id=488050-9901-000&​o=|FS50]] ​ |  625-655 ​nm  |  660 nm  |  665-715 nm  | 
-|  5  |  ​none  ​| ​ ​none  ​ ​none  ​ ​none  ​+|  5  |  ​Empty  ||||
-|  6  |  ​none  ​| ​ ​none  ​ ​none  ​ ​none  ​|+|  6  |  ​Empty  |||||
  
 ==== Objectives ==== ==== Objectives ====
-^  Magnification ​ ^  Model  ^  ​Type  ​^ ​ NA  ^  WD (mm)  ^ +^  Magnification ​ ^  Model  ^  ​Immersion ​ ​^ ​ NA  ^  WD (mm)  ​^ ​ Reference ​ ^ 
-|  10x  |  [[https://​www.micro-shop.zeiss.com/​?​s=3407786617d462&​l=en&​p=uk&​f=o&​a=v&​m=s&​id=420340-9901-000|EC Plan-Neofluar]] ​ |  ​Dry  ​|  ​0.30  ​|  ​5. +|  10x  ​| ​ EC Plan-Neofluar Ph1  |  Air  |  0.30  |  5.20  ​| ​ [[https://​www.micro-shop.zeiss.com/​en/​de/​shop/​objectives/​420341-9911-000/Objective-EC-Plan-Neofluar-10x-0.30-Ph1-M27|420341-9911-000]]  ​
-|  ​20x  ​| ​ [[https://​www.micro-shop.zeiss.com/​?​s=2310860526ae9cb&​l=en&​p=uk&​f=o&​a=v&​m=s&​id=420650-9901-000|Plan-Apochromat]]  |  ​Dry  ​| ​ 0.80  |  0.55  | +|  ​20x  ​|  ​EC Plan-Neofluar Ph 2  ​|  ​Air  |  0.50  ​|  ​2.00  ​| ​ [[https://​www.micro-shop.zeiss.com/​en/​de/​shop/​objectives/​440341-9904-000/​Objective-EC-Plan-Neofluar-20x-0.5-Ph2|440341-9904-000]]  | 
-|  40x  ​| ​ [[https://​www.micro-shop.zeiss.com/​?​s=3407786617d462&​l=en&​p=uk&​f=o&​a=v&​m=s&​id=440350-9903-000|EC Plan-NeoFluar]]  ​|  ​Dry  ​| ​ 0.75  |  0.71  | +|  20x  |  Plan-Apochromat ​ ​|  ​Air  ​| ​ 0.80  |  0.55  |  [[https://​www.micro-shop.zeiss.com/​en/​de/​shop/​objectives/​420650-9901-000/​Objective-Plan-Apochromat-20x-0.8-M27|420650-9901-000]] ​ | 
-|  63x  |  [[https://​www.micro-shop.zeiss.com/​?​s=3407786617d462&​l=en&​p=uk&​f=o&​a=v&​m=s&​id=420782-9900-000|Plan-Apochromat]] ​ |  Oil  |  1.40  |  0.19  |+|  40x  |  ​EC Plan-NeoFluar ​ |  ​Air  ​| ​ 0.75  |  0.71  ​| ​ [[https://​www.micro-shop.zeiss.com/​en/​de/​shop/​objectives/​420360-9900-000/​Objective-EC-Plan-Neofluar-40x-0.75-M27|420360-9900-000]] ​ | 
 +|  63x  ​| ​ Plan-Apochromat DIC  |  Oil  |  1.40  |  0.19  ​| ​ [[https://​www.micro-shop.zeiss.com/​en/​de/​shop/​objectives/​420782-9900-000/Objective-Plan-Apochromat-63x-1.4-Oil-DIC-M27|420782-9900-000]]  | 
 +|  63x  |  Plan-Apochromat Ph3  ​| ​ Oil  |  1.40  |  0.19  ​| ​ [[https://​www.micro-shop.zeiss.com/​en/​de/​shop/​objectives/​420781-9910-000/​Objective-Plan-Apochromat-63-1.4-Oil-Ph3-M27|420781-9910-000]] ​ |
  
 **Upon request:** **Upon request:**
-^  Magnification ​ ^  Model  ^  ​Type  ​^ ​ NA  ^  WD (mm)  ^ +^  Magnification ​ ^  Model  ^  ​Immersion ​ ​^ ​ NA  ^  WD (mm)  ​^ ​ Reference ​ ^ 
-|  1.25x  |  ​[[https://​www.micro-shop.zeiss.com/?​s=3407786617d462&​l=en&​p=uk&​f=o&​a=v&​m=s&​id=422310-9900-000|EC Epiplan-Neofluar]]  ​|  ​Dry  ​| ​ 0.035  |  4  | +|  1.25x  |  EC Epiplan-Neofluar ​ |  ​Air  ​| ​ 0.035  |  4.00  ​| ​ [[https://​www.micro-shop.zeiss.com/​en/​de/​shop/​objectives/​422310-9900-000/​Objective-EC-Epiplan-Neofluar-1.25x-0.03-M27|422310-9900-000]] ​ | 
-|  5x  ​| ​ [[https://​www.micro-shop.zeiss.com/​?​s=571974962233f6&​l=en&​p=uk&​f=o&​a=v&​m=s&​id=420330-9901-000|EC Plan-NeoFluar]]  ​|  ​Dry  ​| ​ 0.16  |  18.5  | +|  5x  |  ​EC Plan-NeoFluar ​ |  ​Air  ​| ​ 0.16  |  18.50  ​| ​ [[https://​www.micro-shop.zeiss.com/​en/​de/​shop/​objectives/​420330-9901-000/Objective-EC-Plan-Neofluar-5x-0.16-M27|420330-9901-000]]  ​
-|  20x  ​| ​ [[https://​www.micro-shop.zeiss.com/​?​s=3407786617d462&​l=en&​p=uk&​f=o&​a=v&​m=s&​id=440341-9904-000|EC Plan-Neofluar ​Ph 2]]  |  ​Dry  ​|  ​0.50  ​|  ​ | +|  ​10x  ​|  ​EC Plan-Neofluar ​ ​|  ​Air  ​| ​ ​0.30  ​|  ​5.20  ​| ​ [[https://​www.micro-shop.zeiss.com/​en/​de/​shop/​objectives/​420340-9901-000/​Objective-EC-Plan-Neofluar-10x-0.30-M27|420340-9901-000]]  ​
-|  ​40x  ​| ​ [[https://​www.micro-shop.zeiss.com/​?​s=129353259d52f12&​l=en&​p=uk&​f=o&​a=v&​m=s&​id=420660-9970-000|Plan-Apochromat]]  |  ​Dry  ​| ​ 0.95  |  0.25  | +|  ​40x  |  Plan-Apochromat Corr  |  Air  ​| ​ 0.95  |  0.25  |  [[https://​www.micro-shop.zeiss.com/​en/​de/​shop/​objectives/​420660-9970-000/​Objective-Plan-Apochromat-40x-0.95-Corr-M27|420660-9970-000]] ​ | 
-|  40x  ​| ​ [[https://​www.micro-shop.zeiss.com/​?​s=3407786617d462&​l=en&​p=uk&​f=o&​a=v&​m=s&​id=440451-9903-000|Plan-Neofluar Ph3]]  ​| ​ Oil  |  1.30  |  0.20  | +|  40x  |  ​Plan-Neofluar Ph3  |  Oil  |  1.30  |  0.20  |  [[https://​www.micro-shop.zeiss.com/​en/​de/​shop/​objectives/​440451-9903-000/​Objective-EC-Plan-Neofluar-40x-1.30-Oil-Ph3|440451-9903-000]]  | 
-|  63x  ​| ​ [[https://​www.micro-shop.zeiss.com/​?​s=571858182d6d9d&​l=en&​p=uk&​f=o&​a=v&​m=s&​id=420781-9910-000|Plan-Apochromat ​Ph3]]  |  Oil  |  1. ​| ​ 0.19  | +|  100x  |  Plan-Apochromat DIC  ​| ​ Oil  |  1.40  ​| ​ 0.17  ​| ​ [[https://​www.micro-shop.zeiss.com/​en/​de/​shop/​objectives/​440782-9902-000/Objective-Plan-Apochromat-100x-1.40-Oil-DIC|440782-9902-000]] ​ |
-|  100x  ​| ​ [[https://​www.micro-shop.zeiss.com/​?​s=3407786617d462&​l=en&​p=uk&​f=o&​a=v&​m=s&​id=440782-9902-000|Plan-Apochromat]]  |  Oil  |  ​1.40   ​0.17 ​ |+
  
 +==== Camera ====
 +^  Model  ^  Type  ^  Frame Size  ^  Pixel Size (µm)  ^
 +|  [[https://​www.zeiss.com/​microscopy/​int/​products/​microscope-cameras/​axiocam-506-mono.html|Axiocam 506 mono]] ​ |  CCD  |  2752 x 2208  |  4.54 x 4.54  |
 ===== System Turn On Procedures ===== ===== System Turn On Procedures =====
-  * Turn on the HXP 120 fluorescence ​light.+  * Turn on the HXP 120 fluorescent ​light source
 {{zeiss_axio_observer_hxp.jpg?​120|}} {{zeiss_axio_observer_hxp.jpg?​120|}}
-  * Turn on the Temperature ​and CO2 controller (if you need them).+  * Turn on the temperature ​and CO2 controller (if you need them).
 {{zeiss_axio_observer_temp.jpg?​80|}} {{zeiss_axio_observer_temp.jpg?​80|}}
   * If you need CO2, open the valve   * If you need CO2, open the valve
 {{zeiss_axio_observer_co2.jpg?​80|}} {{zeiss_axio_observer_co2.jpg?​80|}}
-  * Turn on the Motorized Stage controller (A)+  * Turn on the motorized stage controller (**A** on remote control)
 {{zeiss_axio_observer_a.jpg?​height=150|}} {{zeiss_axio_observer_a.jpg?​height=150|}}
   * Turn on the Definite Focus controller (if you are going to use it)   * Turn on the Definite Focus controller (if you are going to use it)
 {{zeiss_axio_observer_df.jpg?​height=150|}} {{zeiss_axio_observer_df.jpg?​height=150|}}
-  * Turn on the Microscope ​power supply (B)+  * Turn on the microscope ​power supply (**B** on remote control)
 {{zeiss_axio_observer_b.jpg?​height=150|}} {{zeiss_axio_observer_b.jpg?​height=150|}}
   * Turn on the computer   * Turn on the computer
-  * Log in Windows ​with Cell Observer user account+  * Log in to Windows ​(Biomaging User)
-  * Start the **ZEN 2** software+  * Start the **ZEN** software
  
 ===== Microscope Turn Off procedures ===== ===== Microscope Turn Off procedures =====
-  ​Exit the **ZEN 2** software+If there is another user for this microscope is the __next hour__: 
 +  ​Leave the fluorescent light source on 
 +  ​Clean up immersion objectives 
 +Else: 
 +  ​Exit the ZEN software
   * Turn off the computer (not the UPS!)   * Turn off the computer (not the UPS!)
 {{:​cell_observer_pc_vs_ups.png?​250|}} {{:​cell_observer_pc_vs_ups.png?​250|}}
-  * If you used any __immersion oil in the objectives, clean them up__. +  * Clean up immersion ​objectives 
-  * Turn off the microscope power supply (B)+  * Turn off the microscope power supply (**B** on remote control)
   * Turn off the Definite Focus controller (if used)   * Turn off the Definite Focus controller (if used)
-  * Turn off the Motorized Stage controller (A)+  * Turn off the motorized stage controller (**A** on remote control)
   * Turn off the Temperature and CO2 controller (if used)   * Turn off the Temperature and CO2 controller (if used)
-  * If there'​s __another user__ for this microscope in the __next hour__ leave the mercury __lamp power source ​on, else turn it off__.  +  * Turn off the HXP 120 fluorescent light source
-  * Be extra careful in weekends and at the end of the day to make sure that the mercury lamp is not going to be turned on for an excessive time. +
  
 ---- ----
 [[resources|Back to the Resources page]] [[resources|Back to the Resources page]]
zeiss_cell_observer.1562922468.txt.gz · Last modified: 2019/07/12 11:07 by bioimaging