User Tools

Site Tools


zeiss_cell_observer

Differences

This shows you the differences between two versions of the page.

Link to this comparison view

Both sides previous revision Previous revision
Next revision
Previous revision
zeiss_cell_observer [2019/01/11 17:09]
bioimaging
zeiss_cell_observer [2020/01/17 12:33] (current)
bioimaging [Microscope overview]
Line 1: Line 1:
 <​html>​ <​html>​
-<img src="/​facility/​bioimaging/​lib/​exe/​fetch.php?​media=zeiss_cell_observer_name.jpg">+<img src="/​facility/​bioimaging/​lib/​exe/​fetch.php?​media=zeiss_cell_observer_name_new.png">
 <table style="​border:​0px solid white;">​ <table style="​border:​0px solid white;">​
 <tr style="​border:​0px solid white;">​ <tr style="​border:​0px solid white;">​
 <td style="​border:​0px solid white;">​ <td style="​border:​0px solid white;">​
-<a href="/​facility/​bioimaging/​lib/​exe/​fetch.php?​media=zeiss_axio_observer_wiki.jpg"><​img src="/​facility/​bioimaging/​lib/​exe/​fetch.php?​media=zeiss_axio_observer_wiki.jpg" width=300></​a>​+<a href="/​facility/​bioimaging/​lib/​exe/​fetch.php?​media=zeiss_cell_observer_wiki_new.png"><​img src="/​facility/​bioimaging/​lib/​exe/​fetch.php?​media=zeiss_cell_observer_wiki_new.png" width=300></​a>​
 <td style="​border:​0px solid white;">​ <p style="​line-height:​1.8">​ <td style="​border:​0px solid white;">​ <p style="​line-height:​1.8">​
-<​b>​Location</​b>:​ <a href="/​facility/​bioimaging/​lib/​exe/​fetch.php?​media=zeiss_cell_observer_map.jpg">​Room P3-A-10</​a>​ (<img src="/​facility/​bioimaging/​lib/​exe/​fetch.php?​media=telephone_icon.gif" width=15>​ 47331) <​br> ​+<​b>​Location</​b>:​ <a href="/​facility/​bioimaging/​lib/​exe/​fetch.php?​media=zeiss_cell_observer_map_new.png">​Room P3-A-10</​a>​ (<img src="/​facility/​bioimaging/​lib/​exe/​fetch.php?​media=phone_neg.png" width=15>​ 47331) <​br> ​
 <​b>​Manufacturer</​b>:​ <a href="​http://​www.zeiss.de/​micro"​ target="​_blank">​Carl Zeiss MicroImaging</​a><​br>​ <​b>​Manufacturer</​b>:​ <a href="​http://​www.zeiss.de/​micro"​ target="​_blank">​Carl Zeiss MicroImaging</​a><​br>​
 <​b>​Model</​b>:​ Axio Observer<​br>​ <​b>​Model</​b>:​ Axio Observer<​br>​
Line 28: Line 28:
  
 ===== Microscope overview ===== ===== Microscope overview =====
-The Zeiss Cell Observer is a fully motorized inverted //widefield fluorescence microscope//​ ideal for live-cell imaging applications. It is equipped with a large cage incubator and a small stage incubator for temperature control and CO2 supply and with Definite Focus for hardware focus control during long time-lapse acquisitions. Using the ZEN 2 software you can control the motorized filters, shutters and stage to simultaneously set up multi-color time-lapse imaging experiments in multiple stage positions. Its sensitive cooled Axiocam 506m camera allows you to capture weak fluorescent signals and minimize photobleaching/​photodamage in light sensitive samples. You can also perform Z-stack acquisition and use our [[http://​micro.magnet.fsu.edu/​primer/​digitalimaging/​deconvolution/​deconvolutionhome.html|deconvolution]] server ​[[brainstorm|Brainstorm]] ​to significantly improve image resolution even in thick samples. If don't want to use deconvolution,​ check out one of the confocal systems such as the point scanners [[zeiss_lsm_710|Zeiss LSM 710]] and [[zeiss_lsm_880|Zeiss LSM 880]] or spinning disks [[3i_marianas_sdc|3i Marianas SDC]] and [[zeiss_cell_observer_sd|Zeiss Cell Observer SD]]. If your personal computer cannot handle all the data you collected, check out the [[big_guy|Big Guy]] or [[colossus|Colossus]]. If you have FISH or other fixed fluorescence slides try the [[leica_dm5000b|Leica DM5000B]] instead.+{{  :​widefield_lightpath.png?​0x300}}The Zeiss Cell Observer is a fully motorized inverted //widefield fluorescence microscope//​ ideal for live-cell imaging applications. It is equipped with a large cage incubator and a small stage incubator for temperature control and CO2 supply and with Definite Focus for hardware focus control during long time-lapse acquisitions. Using the ZEN 2 software you can control the motorized filters, shutters and stage to simultaneously set up multi-color time-lapse imaging experiments in multiple stage positions. Its sensitive cooled Axiocam 506m camera allows you to capture weak fluorescent signals and minimize photobleaching/​photodamage in light sensitive samples. You can also perform Z-stack acquisition and use [[https://​huygens.i3s.up.pt/​login.php|PPBI Huygens RM]] [[http://​micro.magnet.fsu.edu/​primer/​digitalimaging/​deconvolution/​deconvolutionhome.html|deconvolution]] server to significantly improve image resolution even in thick samples. If don't want to use deconvolution,​ check out one of the confocal systems such as the point scanners [[zeiss_lsm_710|Zeiss LSM 710]] and [[zeiss_lsm_880|Zeiss LSM 880]] or spinning disks [[3i_marianas_sdc|3i Marianas SDC]] and [[zeiss_cell_observer_sd|Zeiss Cell Observer SD]]. If your personal computer cannot handle all the data you collected, check out the [[big_guy|Big Guy]] or [[colossus|Colossus]]. If you have FISH or other fixed fluorescence slides try the [[leica_dm5000b|Leica DM5000B]] instead.
  
   * **Microscope**:​ [[http://​www.zeiss.com/​microscopy/​en_de/​products/​light-microscopes/​axio-observer-for-biology.html|Zeiss Axio Observer]]   * **Microscope**:​ [[http://​www.zeiss.com/​microscopy/​en_de/​products/​light-microscopes/​axio-observer-for-biology.html|Zeiss Axio Observer]]
   * **Camera**: [[https://​www.zeiss.com/​microscopy/​int/​products/​microscope-cameras/​axiocam-506-mono.html|Axiocam 506 mono CCD]]   * **Camera**: [[https://​www.zeiss.com/​microscopy/​int/​products/​microscope-cameras/​axiocam-506-mono.html|Axiocam 506 mono CCD]]
  
-{{warning.jpg?20|}} Data files older than **1 month** will be automatically deleted on this system, please copy your data to the iMM server using the desktop link.+{{:warning.png?25|}} Data files older than **1 month** will be automatically deleted on this system, please copy your data to the iMM server using the desktop link.
  
 ===== Booking Rules ===== ===== Booking Rules =====
Line 59: Line 59:
  
 ==== Objectives ==== ==== Objectives ====
-^  Magnification ​ ^  Model  ^  ​Type  ​^ ​ NA  ^  WD (mm)  ^ +^  Magnification ​ ^  Model  ^  ​Immersion ​ ​^ ​ NA  ^  WD (mm)  ​^ ​ Reference ​ ^ 
-|  10x  |  [[https://​www.micro-shop.zeiss.com/​?​s=3407786617d462&​l=en&​p=uk&​f=o&​a=v&​m=s&​id=420340-9901-000|EC Plan-Neofluar]] ​ |  ​Dry  ​|  ​0.30  ​|  ​5. +|  10x  ​| ​ EC Plan-Neofluar ​ |  Air  |  0.30  |  5.2  ​| ​ [[https://​www.micro-shop.zeiss.com/​en/​de/​shop/​objectives/​420340-9901-000/Objective-EC-Plan-Neofluar-10x-0.30-M27|420340-9901-000]]  ​
-|  ​20x  ​| ​ [[https://​www.micro-shop.zeiss.com/​?​s=2310860526ae9cb&​l=en&​p=uk&​f=o&​a=v&​m=s&​id=420650-9901-000|Plan-Apochromat]] ​ |  ​Dry  ​|  ​0.80  ​| ​ 0.55  +|  ​20x  ​|  ​Plan-Apochromat ​ ​|  ​Air  |  0.80  ​|  ​0.55  ​| ​ [[https://​www.micro-shop.zeiss.com/​en/​de/​shop/​objectives/​420650-9901-000/Objective-Plan-Apochromat-20x-0.8-M27|420650-9901-000]]  ​
-|  ​40x  ​| ​ [[https://​www.micro-shop.zeiss.com/​?​s=3407786617d462&​l=en&​p=uk&​f=o&​a=v&​m=s&​id=440350-9903-000|EC Plan-NeoFluar]]  |  ​Dry  ​|  ​0.75  ​|  ​0.71  +|  ​40x  ​|  ​EC Plan-NeoFluar ​ |  Air  ​| ​ 0.75  ​|  ​0.71  ​| ​ [[https://​www.micro-shop.zeiss.com/​en/​de/​shop/​objectives/​440350-9903-000/Objective-EC-Plan-Neofluar-40x-0.75|440350-9903-000]]  ​
-|  ​63x  ​| ​ [[https://​www.micro-shop.zeiss.com/​?​s=3407786617d462&​l=en&​p=uk&​f=o&​a=v&​m=s&​id=420782-9900-000|Plan-Apochromat]]  |  Oil  |  ​1.40  ​ ​0.19 ​ |+|  ​63x  ​|  ​Plan-Apochromat DIC  ​|  ​Oil  |  1.40  ​|  ​0.19  ​| ​ [[https://​www.micro-shop.zeiss.com/​en/​de/​shop/​objectives/​420782-9900-000/Objective-Plan-Apochromat-63x-1.4-Oil-DIC-M27|420782-9900-000]] ​ |
  
 **Upon request:** **Upon request:**
-^  Magnification ​ ^  Model  ^  ​Type  ​^ ​ NA  ^  WD (mm)  ^ +^  Magnification ​ ^  Model  ^  ​Immersion ​ ​^ ​ NA  ^  WD (mm)  ​^ ​ Reference ​ ^ 
-|  1.25x  |  [[https://​www.micro-shop.zeiss.com/​?​s=3407786617d462&​l=en&​p=uk&​f=o&​a=v&​m=s&​id=422310-9900-000|EC Epiplan-Neofluar]] ​ |  ​Dry  ​|  ​0.035  ​|  ​ | +|  1.25x  ​| ​ EC Epiplan-Neofluar ​ |  Air  |  0.035  |  4  ​| ​ [[https://​www.micro-shop.zeiss.com/​en/​de/​shop/​objectives/​422310-9900-000/Objective-EC-Epiplan-Neofluar-1.25x-0.03-M27|422310-9900-000]]  ​
-|  ​5x  ​| ​ [[https://​www.micro-shop.zeiss.com/​?​s=571974962233f6&​l=en&​p=uk&​f=o&​a=v&​m=s&​id=420330-9901-000|EC Plan-NeoFluar]]  |  ​Dry  ​|  ​0.16  ​|  ​18. +|  ​5x  ​|  ​EC Plan-NeoFluar ​ ​|  ​Air  ​| ​ ​0.16  ​|  ​18.5  ​| ​ [[https://​www.micro-shop.zeiss.com/​en/​de/​shop/​objectives/​420330-9901-000/Objective-EC-Plan-Neofluar-5x-0.16-M27|420330-9901-000]]  ​
-|  ​20x  ​| ​ [[https://​www.micro-shop.zeiss.com/​?​s=3407786617d462&​l=en&​p=uk&​f=o&​a=v&​m=s&​id=440341-9904-000|EC Plan-Neofluar ​Ph 2]]  |  ​Dry  ​|  ​0.50  ​|  ​ | +|  ​20x  ​|  ​EC Plan-Neofluar Ph 2  ​|  ​Air  |  0.50  ​|  ​ ​| ​ [[https://​www.micro-shop.zeiss.com/​en/​de/​shop/​objectives/​440341-9904-000/Objective-EC-Plan-Neofluar-20x-0.5-Ph2|440341-9904-000]]  ​
-|  ​40x  ​| ​ [[https://​www.micro-shop.zeiss.com/​?​s=129353259d52f12&​l=en&​p=uk&​f=o&​a=v&​m=s&​id=420660-9970-000|Plan-Apochromat]] ​ |  ​Dry  ​|  ​0.95  ​|  ​0.25  +|  ​40x  ​|  ​Plan-Apochromat Corr  ​|  ​Air  ​| ​ ​0.95  ​|  ​0.25  ​| ​ [[https://​www.micro-shop.zeiss.com/​en/​de/​shop/​objectives/​420660-9970-000/Objective-Plan-Apochromat-40x-0.95-Corr-M27|420660-9970-000]]  ​
-|  ​40x  ​| ​ [[https://​www.micro-shop.zeiss.com/​?​s=3407786617d462&​l=en&​p=uk&​f=o&​a=v&​m=s&​id=440451-9903-000|Plan-Neofluar Ph3]]  |  Oil  |  1.30  ​| ​ 0.20  | +|  ​40x  ​|  ​Plan-Neofluar Ph3  ​|  ​Oil  |  1.30  ​|  ​0.20  ​| ​ [[https://​www.micro-shop.zeiss.com/​en/​de/​shop/​objectives/​440451-9903-000/​Objective-EC-Plan-Neofluar-40x-1.30-Oil-Ph3|440451-9903-000]]  | 
-|  63x  ​| ​ [[https://​www.micro-shop.zeiss.com/​?​s=571858182d6d9d&​l=en&​p=uk&​f=o&​a=v&​m=s&​id=420781-9910-000|Plan-Apochromat Ph3]]  |  Oil  |  1. ​| ​ 0.19  | +|  63x  |  Plan-Apochromat Ph3  ​| ​ Oil  |  1. ​| ​ 0.19  ​| ​ [[https://​www.micro-shop.zeiss.com/​en/​de/​shop/​objectives/​420781-9910-000/Objective-Plan-Apochromat-63-1.4-Oil-Ph3-M27|420781-9910-000]]  | 
-|  100x  ​| ​ [[https://​www.micro-shop.zeiss.com/​?​s=3407786617d462&​l=en&​p=uk&​f=o&​a=v&​m=s&​id=440782-9902-000|Plan-Apochromat]]  |  Oil  |  ​1.40   ​0.17 ​ |+|  100x  |  Plan-Apochromat DIC  ​| ​ Oil  |  1.40  ​| ​ 0.17  ​| ​ [[https://​www.micro-shop.zeiss.com/​en/​de/​shop/​objectives/​440782-9902-000/Objective-Plan-Apochromat-100x-1.40-Oil-DIC|440782-9902-000]] ​ |
  
 +==== Camera ====
 +^  Model  ^  Type  ^  Frame Size  ^  Pixel Size (µm)  ^
 +|  [[https://​www.zeiss.com/​microscopy/​int/​products/​microscope-cameras/​axiocam-506-mono.html|Axiocam 506 mono]] ​ |  CCD  |  2752 x 2208  |  4.54 x 4.54  |
 ===== System Turn On Procedures ===== ===== System Turn On Procedures =====
-  * Turn on the HXP 120 fluorescence ​light.+  * Turn on the HXP 120 fluorescent ​light source
 {{zeiss_axio_observer_hxp.jpg?​120|}} {{zeiss_axio_observer_hxp.jpg?​120|}}
-  * Turn on the Temperature ​and CO2 controller (if you need them).+  * Turn on the temperature ​and CO2 controller (if you need them).
 {{zeiss_axio_observer_temp.jpg?​80|}} {{zeiss_axio_observer_temp.jpg?​80|}}
   * If you need CO2, open the valve   * If you need CO2, open the valve
 {{zeiss_axio_observer_co2.jpg?​80|}} {{zeiss_axio_observer_co2.jpg?​80|}}
-  * Turn on the Motorized Stage controller (A)+  * Turn on the motorized stage controller (**A** on remote control)
 {{zeiss_axio_observer_a.jpg?​height=150|}} {{zeiss_axio_observer_a.jpg?​height=150|}}
   * Turn on the Definite Focus controller (if you are going to use it)   * Turn on the Definite Focus controller (if you are going to use it)
 {{zeiss_axio_observer_df.jpg?​height=150|}} {{zeiss_axio_observer_df.jpg?​height=150|}}
-  * Turn on the Microscope ​power supply (B)+  * Turn on the microscope ​power supply (**B** on remote control)
 {{zeiss_axio_observer_b.jpg?​height=150|}} {{zeiss_axio_observer_b.jpg?​height=150|}}
   * Turn on the computer   * Turn on the computer
-  * Log in Windows ​with Cell Observer user account+  * Log in to Windows ​(Biomaging User)
-  * Start the **ZEN 2** software+  * Start the **ZEN** software
  
 ===== Microscope Turn Off procedures ===== ===== Microscope Turn Off procedures =====
-  ​Exit the **ZEN 2** software +If there is another user for this microscope is the __next hour__: 
-  * Turn off the computer +  ​Leave the fluorescent light source on 
-  * If you used any __immersion oil in the objectives, clean them up__. +  ​Clean up immersion objectives 
-  * Turn off the microscope power supply (B)+Else: 
 +  ​Exit the ZEN software 
 +  * Turn off the computer ​(not the UPS!) 
 +{{:​cell_observer_pc_vs_ups.png?​250|}} 
 +  * Clean up immersion ​objectives 
 +  * Turn off the microscope power supply (**B** on remote control)
   * Turn off the Definite Focus controller (if used)   * Turn off the Definite Focus controller (if used)
-  * Turn off the Motorized Stage controller (A)+  * Turn off the motorized stage controller (**A** on remote control)
   * Turn off the Temperature and CO2 controller (if used)   * Turn off the Temperature and CO2 controller (if used)
-  * If there'​s __another user__ for this microscope in the __next hour__ leave the mercury __lamp power source ​on, else turn it off__.  +  * Turn off the HXP 120 fluorescent light source
-  * Be extra careful in weekends and at the end of the day to make sure that the mercury lamp is not going to be turned on for an excessive time. +
  
 ---- ----
 [[resources|Back to the Resources page]] [[resources|Back to the Resources page]]
zeiss_cell_observer.1547222971.txt.gz · Last modified: 2019/01/11 17:09 by bioimaging