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zeiss_cell_observer [2021/06/25 20:52] bioimaging |
zeiss_cell_observer [2022/01/20 13:26] bioimaging |
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<b>Model</b>: Axio Observer<br> | <b>Model</b>: Axio Observer<br> | ||
<b>Nickname</b>: "Cell Observer"<br> | <b>Nickname</b>: "Cell Observer"<br> | ||
- | <b>Software</b>: ZEN 2 (blue edition)<br> | + | <b>Software</b>: ZEN 3.0 (blue edition)<br> |
<b>Year</b>: 2015 <br> | <b>Year</b>: 2015 <br> | ||
<b>SN</b>: 3851002396<br> | <b>SN</b>: 3851002396<br> | ||
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===== System Turn On Procedures ===== | ===== System Turn On Procedures ===== | ||
- | * Select LED or HXP light source on the manual path deflecting mirror (in the back of the microscope) | ||
- | {{:cell_observer_led_selected.png?150|}} {{:cell_observer_hxp_selected.png?150|}} | ||
* Turn on the HXP 120 fluorescent light source (if used) | * Turn on the HXP 120 fluorescent light source (if used) | ||
{{zeiss_axio_observer_hxp.jpg?120|}} | {{zeiss_axio_observer_hxp.jpg?120|}} | ||
- | * Turn on the temperature and CO2 controller (if you need them). | + | * Turn on the temperature and CO2 controller - white power strip (if you need them). |
- | {{zeiss_axio_observer_temp.jpg?80|}} | + | |
* If you need CO2, open the Cell Observer CO2 valve | * If you need CO2, open the Cell Observer CO2 valve | ||
* Turn on the motorized stage controller (**A** on remote control) | * Turn on the motorized stage controller (**A** on remote control) | ||
{{zeiss_axio_observer_a.jpg?height=150|}} | {{zeiss_axio_observer_a.jpg?height=150|}} | ||
- | * Turn on the Definite Focus controller (if you are going to use it) | ||
- | {{zeiss_axio_observer_df.jpg?height=150|}} | ||
* Turn on the microscope power supply (**B** on remote control) | * Turn on the microscope power supply (**B** on remote control) | ||
{{zeiss_axio_observer_b.jpg?height=150|}} | {{zeiss_axio_observer_b.jpg?height=150|}} | ||
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* Select HXP or LED Workspace in ZEN (top left) | * Select HXP or LED Workspace in ZEN (top left) | ||
{{::workspace_selection.png?250|}} | {{::workspace_selection.png?250|}} | ||
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- | **Warning**: If you need to change the stage adapter, please contact the <html><a href="/facility/bioimaging/doku.php?id=about">Bioimaging Unit</a> (<b>imm-bioimaging@medicina.ulisboa.pt</b> | <img src="/facility/bioimaging/lib/exe/fetch.php?media=phone_neg.png" width=15> 47222</html>) | ||
===== Microscope Turn Off procedures ===== | ===== Microscope Turn Off procedures ===== |