zeiss_cell_observer
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zeiss_cell_observer [2020/12/02 12:29] bioimaging |
zeiss_cell_observer [2021/01/17 04:44] bioimaging [Microscope Turn Off procedures] |
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| | 1 | HE GFP | [[https://www.micro-shop.zeiss.com/?s=23108437692794b&l=en&p=us&f=f&a=v&b=f&id=489038-9901-000&o=|FS38HE]] | 450-490 nm | 495 nm | 500-550 nm | | | 1 | HE GFP | [[https://www.micro-shop.zeiss.com/?s=23108437692794b&l=en&p=us&f=f&a=v&b=f&id=489038-9901-000&o=|FS38HE]] | 450-490 nm | 495 nm | 500-550 nm | | ||
| | 2 | HE DsRed | [[https://www.micro-shop.zeiss.com/?s=23108437692794b&l=en&p=us&f=f&a=v&b=f&id=489043-9901-000&o=|FS43HE]] | 538-562 nm | 570 nm | 570-640 nm | | | 2 | HE DsRed | [[https://www.micro-shop.zeiss.com/?s=23108437692794b&l=en&p=us&f=f&a=v&b=f&id=489043-9901-000&o=|FS43HE]] | 538-562 nm | 570 nm | 570-640 nm | | ||
| - | | 3 | DAPI | [[https://www.micro-shop.zeiss.com/?s=23108437692794b&l=en&p=us&f=f&a=v&b=f&id=488049-9901-000&o=|FS49]] | 335-383 nm | 395 nm | 420-470 nm | | + | | 3 | Cy5 | [[https://www.micro-shop.zeiss.com/?s=23108437692794b&l=en&p=us&f=f&a=v&b=f&id=488050-9901-000&o=|FS50]] | 625-655 nm | 660 nm | 665-715 nm | |
| - | | 4 | Cy5 | [[https://www.micro-shop.zeiss.com/?s=23108437692794b&l=en&p=us&f=f&a=v&b=f&id=488050-9901-000&o=|FS50]] | 625-655 nm | 660 nm | 665-715 nm | | + | | 4 | Analyzer DIC ||||| |
| | 5 | QUAD | [[https://www.micro-shop.zeiss.com/en/us/shop/filterAssistant/filtersets/489090-9100-000|FS90HE]] | 370-400 nm | 405 nm | 410-440 nm | | | 5 | QUAD | [[https://www.micro-shop.zeiss.com/en/us/shop/filterAssistant/filtersets/489090-9100-000|FS90HE]] | 370-400 nm | 405 nm | 410-440 nm | | ||
| | ::: | ::: | ::: | 450-488 nm | 493 nm | 499-529 nm | | | ::: | ::: | ::: | 450-488 nm | 493 nm | 499-529 nm | | ||
| | ::: | ::: | ::: | 540-570 nm | 575 nm | 580-605 nm | | | ::: | ::: | ::: | 540-570 nm | 575 nm | 580-605 nm | | ||
| | ::: | ::: | ::: | 615-648 nm | 653 nm | 659-759 nm | | | ::: | ::: | ::: | 615-648 nm | 653 nm | 659-759 nm | | ||
| - | | 6 | Empty ||||| | + | | 6 | DAPI | [[https://www.micro-shop.zeiss.com/?s=23108437692794b&l=en&p=us&f=f&a=v&b=f&id=488049-9901-000&o=|FS49]] | 335-383 nm | 395 nm | 420-470 nm | |
| ==== Objectives ==== | ==== Objectives ==== | ||
| Line 82: | Line 82: | ||
| * Turn on the temperature and CO2 controller (if you need them). | * Turn on the temperature and CO2 controller (if you need them). | ||
| {{zeiss_axio_observer_temp.jpg?80|}} | {{zeiss_axio_observer_temp.jpg?80|}} | ||
| - | * If you need CO2, open the valve | + | * If you need CO2, open the main valve and the Cell Observer CO2 valve |
| - | {{zeiss_axio_observer_co2.jpg?80|}} | + | {{::p3a10_co2_valves.png?200|}} |
| * Turn on the motorized stage controller (**A** on remote control) | * Turn on the motorized stage controller (**A** on remote control) | ||
| {{zeiss_axio_observer_a.jpg?height=150|}} | {{zeiss_axio_observer_a.jpg?height=150|}} | ||
| Line 109: | Line 109: | ||
| * Turn off the motorized stage controller (**A** on remote control) | * Turn off the motorized stage controller (**A** on remote control) | ||
| * Turn off the Temperature and CO2 controller (if used) | * Turn off the Temperature and CO2 controller (if used) | ||
| + | * Close the Cell Observer CO2 valve (if used) | ||
| * Turn off the HXP 120 fluorescent light source | * Turn off the HXP 120 fluorescent light source | ||
| ---- | ---- | ||
| - | [[resources|Back to the Resources page]] | + | [[resources|Back to the Equipment page]] |
zeiss_cell_observer.txt · Last modified: 2024/04/01 23:50 by bioimaging
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