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zeiss_axiovert_200m [2021/01/17 04:45]
bioimaging [Microscope Turn Off procedures]
zeiss_axiovert_200m [2022/02/14 22:16] (current)
bioimaging [Microscope overview]
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 <a href="/​facility/​bioimaging/​lib/​exe/​fetch.php?​media=zeiss_axiovert_200m_wiki_new.png"><​img src="/​facility/​bioimaging/​lib/​exe/​fetch.php?​media=zeiss_axiovert_200m_wiki_new.png"​ width=300></​a>​ <a href="/​facility/​bioimaging/​lib/​exe/​fetch.php?​media=zeiss_axiovert_200m_wiki_new.png"><​img src="/​facility/​bioimaging/​lib/​exe/​fetch.php?​media=zeiss_axiovert_200m_wiki_new.png"​ width=300></​a>​
 <td style="​border:​0px solid white;">​ <p style="​line-height:​1.8">​ <td style="​border:​0px solid white;">​ <p style="​line-height:​1.8">​
-<​b>​Location</​b>:​ <a href="/​facility/​bioimaging/​lib/​exe/​fetch.php?​media=zeiss_axiovert_200m_map_new.png">​Room P2-A-22</a> (<img src="/​facility/​bioimaging/​lib/​exe/​fetch.php?​media=phone_neg.png"​ width=15> ​47217) <​br> ​+<​b>​Location</​b>:​ <a href="/​facility/​bioimaging/​lib/​exe/​fetch.php?​media=zeiss_axiovert_200m_map_new_p2a24.png">​Room P2-A-24</a> (<img src="/​facility/​bioimaging/​lib/​exe/​fetch.php?​media=phone_neg.png"​ width=15> ​47219) <​br> ​
 <​b>​Manufacturer</​b>:​ <a href="​http://​www.zeiss.de/​micro"​ target="​_blank">​Carl Zeiss MicroImaging</​a><​br>​ <​b>​Manufacturer</​b>:​ <a href="​http://​www.zeiss.de/​micro"​ target="​_blank">​Carl Zeiss MicroImaging</​a><​br>​
 <​b>​Model</​b>:​ Axiovert 200M<​br>​ <​b>​Model</​b>:​ Axiovert 200M<​br>​
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 ===== Microscope overview ===== ===== Microscope overview =====
  
-{{  :​widefield_lightpath.png?​0x300}}The Zeiss Axiovert 200M is a fully motorized inverted //widefield fluorescence microscope//​ ideal for live-cell imaging applications. Using the Metamorph software you can control the motorized filters, shutters and stage to simultaneously set up multi-color time-lapse imaging experiments in multiple stage positions. Its sensitive cooled CCD camera (Photometrics CoolSNAP HQ CCD) allows you to capture weak fluorescent signals and minimize photobleaching/​photodamage in light sensitive samples. You can also perform Z-stack acquisition and use [[https://​huygens.i3s.up.pt/​login.php|PPBI Huygens RM]] [[http://​micro.magnet.fsu.edu/​primer/​digitalimaging/​deconvolution/​deconvolutionhome.html|deconvolution]] server to significantly improve image resolution even in thick samples. If you are performing long time-lapse experiments in live samples (aqueous media) and need hardware focus control, check the [[zeiss_cell_observer|Zeiss Cell Observer]] with Definite Focus or the [[nikon_eclipse_ti|Nikon Eclipse Ti]]. If don't want to use deconvolution but need optical sectioning, check out one of the confocal systems such as the point scanners [[zeiss_lsm_710|Zeiss LSM 710]] and [[zeiss_lsm_880|Zeiss LSM 880]] or the spinning disks [[3i_marianas_sdc|3i Marianas SDC]] and [[zeiss_cell_observer_sd|Zeiss Cell Observer SD]]. If your personal computer cannot handle all the data you collected, check out the [[big_guy|Big Guy]] or [[colossus|Colossus]]. If you have FISH or other fixed fluorescence slides try the [[leica_dm5000b|Leica DM5000B]] instead.+{{  :​widefield_lightpath.png?​0x300}}The Zeiss Axiovert 200M is a fully motorized inverted //widefield fluorescence microscope//​ ideal for live-cell imaging applications. Using the Metamorph software you can control the motorized filters, shutters and stage to simultaneously set up multi-color time-lapse imaging experiments in multiple stage positions. Its sensitive cooled CCD camera (Photometrics CoolSNAP HQ CCD) allows you to capture weak fluorescent signals and minimize photobleaching/​photodamage in light sensitive samples. You can also perform Z-stack acquisition and use [[https://​huygens.i3s.up.pt/​login.php|PPBI Huygens RM]] [[http://​micro.magnet.fsu.edu/​primer/​digitalimaging/​deconvolution/​deconvolutionhome.html|deconvolution]] server to significantly improve image resolution even in thick samples. If you are performing long time-lapse experiments in live samples (aqueous media) and need hardware focus control, check the [[zeiss_cell_observer|Zeiss Cell Observer]] with Definite Focus or the [[nikon_eclipse_ti|Nikon Eclipse Ti]]. If don't want to use deconvolution but need optical sectioning, check out one of the confocal systems such as the point scanners [[zeiss_lsm_710|Zeiss LSM 710]] and [[zeiss_lsm_880|Zeiss LSM 880]] or the spinning disks [[3i_marianas_sdc|3i Marianas SDC]] and [[zeiss_cell_observer_sd|Zeiss Cell Observer SD]]. If your personal computer cannot handle all the data you collected, check out the [[colossus|Colossus]]. If you have FISH or other fixed fluorescence slides try the [[leica_dm5000b|Leica DM5000B]] instead.
  
   * **Microscope**:​ Zeiss Axiovert 200   * **Microscope**:​ Zeiss Axiovert 200
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 |  40x  |  EC Plan-NeoFluar ​ |  Air  |  0.75  |  0.71  |  0.24  |  41 pixels ​ |  93.7 pixels ​ |  [[https://​www.micro-shop.zeiss.com/​en/​de/​shop/​objectives/​440350-9903-000/​Objective-EC-Plan-Neofluar-40x-0.75|440350-9903-000]] ​ | |  40x  |  EC Plan-NeoFluar ​ |  Air  |  0.75  |  0.71  |  0.24  |  41 pixels ​ |  93.7 pixels ​ |  [[https://​www.micro-shop.zeiss.com/​en/​de/​shop/​objectives/​440350-9903-000/​Objective-EC-Plan-Neofluar-40x-0.75|440350-9903-000]] ​ |
 |  63x  |  Plan-Apochromat DIC  |  Oil  |  1.40  |  0.19  |  0.15  |  65 pixels ​ |  -  |  [[https://​www.micro-shop.zeiss.com/​en/​de/​shop/​objectives/​420782-9900-000/​Objective-Plan-Apochromat-63x-1.4-Oil-DIC-M27|420782-9900-000]] ​ | |  63x  |  Plan-Apochromat DIC  |  Oil  |  1.40  |  0.19  |  0.15  |  65 pixels ​ |  -  |  [[https://​www.micro-shop.zeiss.com/​en/​de/​shop/​objectives/​420782-9900-000/​Objective-Plan-Apochromat-63x-1.4-Oil-DIC-M27|420782-9900-000]] ​ |
 +|  100x  |  α Plan-Apochromat DIC  |  Oil  |  1.46  |  0.11  |  0.09  |  105 pixels ​ |  -  |  [[https://​www.micro-shop.zeiss.com/​en/​de/​shop/​objectives/​420792-9800-000/​Objective-alpha-Plan-Apochromat-100x-1.46-Oil-DIC-M27|420792-9800-000]] ​ |
  
  
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 |  40x  |  Plan-Neofluar Ph3  |  Oil  |  1.30  |  0.20  |  0.24  |  41 pixels ​ |  [[https://​www.micro-shop.zeiss.com/​en/​de/​shop/​objectives/​440451-9903-000/​Objective-EC-Plan-Neofluar-40x-1.30-Oil-Ph3|440451-9903-000]] ​ | |  40x  |  Plan-Neofluar Ph3  |  Oil  |  1.30  |  0.20  |  0.24  |  41 pixels ​ |  [[https://​www.micro-shop.zeiss.com/​en/​de/​shop/​objectives/​440451-9903-000/​Objective-EC-Plan-Neofluar-40x-1.30-Oil-Ph3|440451-9903-000]] ​ |
 |  63x  |  Plan-Apochromat Ph3  |  Oil  |  1.4  |  0.19  |  0.15  |  65 pixels ​ |  [[https://​www.micro-shop.zeiss.com/​en/​de/​shop/​objectives/​420781-9910-000/​Objective-Plan-Apochromat-63-1.4-Oil-Ph3-M27|420781-9910-000]] ​ | |  63x  |  Plan-Apochromat Ph3  |  Oil  |  1.4  |  0.19  |  0.15  |  65 pixels ​ |  [[https://​www.micro-shop.zeiss.com/​en/​de/​shop/​objectives/​420781-9910-000/​Objective-Plan-Apochromat-63-1.4-Oil-Ph3-M27|420781-9910-000]] ​ |
-|  100x  |  α Plan-Apochromat DIC  |  Oil  |  1.46  |  0.11  |  0.09  |  105 pixels ​ |  [[https://​www.micro-shop.zeiss.com/​en/​de/​shop/​objectives/​420792-9800-000/​Objective-alpha-Plan-Apochromat-100x-1.46-Oil-DIC-M27|420792-9800-000]] ​ | 
  
 *** pixel sizes for binning = 1** *** pixel sizes for binning = 1**
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 ===== System Turn On Procedures ===== ===== System Turn On Procedures =====
-  * Turn on the fluorescent light power source+  * Turn on the fluorescent light power source ​(if used)
 {{halide_710.jpg?​80|}} {{halide_710.jpg?​80|}}
   * Turn on the Prior stage controller ​   * Turn on the Prior stage controller ​
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 If there is another user for this microscope in the __next hour__: If there is another user for this microscope in the __next hour__:
-  * Leave the fluorescent lamp on+  * Leave the fluorescent lamp on (if used)
   * Clean up immersion objectives   * Clean up immersion objectives
 Else: Else:
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   * Turn off the Prior stage controller   * Turn off the Prior stage controller
   * Turn off the microscope   * Turn off the microscope
-  * Turn off the fluorescent lamp+  * Turn off the fluorescent lamp (if used)
  
 ---- ----
 [[resources|Back to the Equipment page]] [[resources|Back to the Equipment page]]
zeiss_axiovert_200m.1610855114.txt.gz · Last modified: 2021/01/17 04:45 by bioimaging