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zeiss_axiovert_200m [2020/01/20 23:39]
bioimaging
zeiss_axiovert_200m [2020/07/21 23:27] (current)
bioimaging
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 &nbsp <br> &nbsp <br>
 &nbsp <br> &nbsp <br>
-&rarr; <a href="​https://​imm.medicina.ulisboa.pt/​intranet/booking/3"><​img src="/​facility/​bioimaging/​lib/​exe/​fetch.php?​media=date.png"> ​ Zeiss Axiovert 200M Booking</​a>​+&rarr; <a href="​https://​my.agendo.science/calendar/?​id=MjhjYWxlbmRhcnM="><​img src="/​facility/​bioimaging/​lib/​exe/​fetch.php?​media=date.png"> ​ Zeiss Axiovert 200M Booking</​a>​
  <​br> ​  <​br> ​
 &rarr; <a href="/​facility/​bioimaging/​doku.php?​id=zeiss_axiovert200m_quality"><​img src="/​facility/​bioimaging/​lib/​exe/​fetch.php?​media=list-icon.png"​ width=18>​ Zeiss Axiovert 200M Quality Control</​a>​ &rarr; <a href="/​facility/​bioimaging/​doku.php?​id=zeiss_axiovert200m_quality"><​img src="/​facility/​bioimaging/​lib/​exe/​fetch.php?​media=list-icon.png"​ width=18>​ Zeiss Axiovert 200M Quality Control</​a>​
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 ===== Microscope overview ===== ===== Microscope overview =====
  
-{{  :​widefield_lightpath.png?​0x300}}The Zeiss Axiovert 200M is a fully motorized inverted //widefield fluorescence microscope//​ ideal for live-cell imaging applications. Using the Metamorph software you can control the motorized filters, shutters and stage to simultaneously set up multi-color time-lapse imaging experiments in multiple stage positions. Its sensitive cooled CCD camera (Photometrics ​Coolsnap ​HQ CCD) allows you to capture weak fluorescent signals and minimize photobleaching/​photodamage in light sensitive samples. You can also perform Z-stack acquisition and use [[https://​huygens.i3s.up.pt/​login.php|PPBI Huygens RM]] [[http://​micro.magnet.fsu.edu/​primer/​digitalimaging/​deconvolution/​deconvolutionhome.html|deconvolution]] server to significantly improve image resolution even in thick samples. If you are performing long time-lapse experiments in live samples (aqueous media) and need hardware focus control, check the [[zeiss_cell_observer|Zeiss Cell Observer]] with Definite Focus or the [[nikon_eclipse_ti|Nikon Eclipse Ti]]. If don't want to use deconvolution but need optical sectioning, check out one of the confocal systems such as the point scanners [[zeiss_lsm_710|Zeiss LSM 710]] and [[zeiss_lsm_880|Zeiss LSM 880]] or the spinning disks [[3i_marianas_sdc|3i Marianas SDC]] and [[zeiss_cell_observer_sd|Zeiss Cell Observer SD]]. If your personal computer cannot handle all the data you collected, check out the [[big_guy|Big Guy]] or [[colossus|Colossus]]. If you have FISH or other fixed fluorescence slides try the [[leica_dm5000b|Leica DM5000B]] instead.+{{  :​widefield_lightpath.png?​0x300}}The Zeiss Axiovert 200M is a fully motorized inverted //widefield fluorescence microscope//​ ideal for live-cell imaging applications. Using the Metamorph software you can control the motorized filters, shutters and stage to simultaneously set up multi-color time-lapse imaging experiments in multiple stage positions. Its sensitive cooled CCD camera (Photometrics ​CoolSNAP ​HQ CCD) allows you to capture weak fluorescent signals and minimize photobleaching/​photodamage in light sensitive samples. You can also perform Z-stack acquisition and use [[https://​huygens.i3s.up.pt/​login.php|PPBI Huygens RM]] [[http://​micro.magnet.fsu.edu/​primer/​digitalimaging/​deconvolution/​deconvolutionhome.html|deconvolution]] server to significantly improve image resolution even in thick samples. If you are performing long time-lapse experiments in live samples (aqueous media) and need hardware focus control, check the [[zeiss_cell_observer|Zeiss Cell Observer]] with Definite Focus or the [[nikon_eclipse_ti|Nikon Eclipse Ti]]. If don't want to use deconvolution but need optical sectioning, check out one of the confocal systems such as the point scanners [[zeiss_lsm_710|Zeiss LSM 710]] and [[zeiss_lsm_880|Zeiss LSM 880]] or the spinning disks [[3i_marianas_sdc|3i Marianas SDC]] and [[zeiss_cell_observer_sd|Zeiss Cell Observer SD]]. If your personal computer cannot handle all the data you collected, check out the [[big_guy|Big Guy]] or [[colossus|Colossus]]. If you have FISH or other fixed fluorescence slides try the [[leica_dm5000b|Leica DM5000B]] instead.
  
   * **Microscope**:​ Zeiss Axiovert 200   * **Microscope**:​ Zeiss Axiovert 200
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 {{:​warning.png?​25|}} Data files older than **1 month** will be automatically deleted on this system, please copy your data to the iMM server using the desktop link. {{:​warning.png?​25|}} Data files older than **1 month** will be automatically deleted on this system, please copy your data to the iMM server using the desktop link.
- 
-===== Booking Rules ===== 
-The Axiovert 200M booking is divided in three daily slots: 
-|  9:00 to 13:00  |  13:00 to 17:00  |  17:00 to 21:00  | 
- 
-  - A **user** can book at maximum **2 sessions per week** 
-  - Each **session must fit inside one of the three daily slots** (i.e. a session booked from 11:00 to 14:00 counts as 2 sessions) 
-  - The above rules do not apply for **weekends** and for work days **before 9:00** and **after 21:00** 
-  - The above rules do not apply for sessions booked on the very same day or **24 hours ahead** 
-  - Exceptions to these rules require **approval from [[joserino@medicina.ulisboa.pt|José Rino]]**. 
  
 ===== System components ===== ===== System components =====
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 ==== Filtersets ==== ==== Filtersets ====
  
-^  Position ​ ^  Filterset ​ ^  Reference ​ ^  Excitation ​ ^  Emission ​ ^ +^  Position ​ ^  Filterset ​ ^  Reference ​ ^  Excitation ​ ​^ ​ Dichroic ​ ​^ ​ Emission ​ ^ 
-|  1  |  Blue  |  [[https://​www.micro-shop.zeiss.com/?​s=163176628fcf844&​l=en&​p=us&​f=f&​a=v&​b=f&​id=488001-9901-000&​o=|FS01]] ​ |  359-371 nm  |  > 397 nm  | +|  1  |  Blue  |  [[https://​www.micro-shop.zeiss.com/?​s=163176628fcf844&​l=en&​p=us&​f=f&​a=v&​b=f&​id=488001-9901-000&​o=|FS01]] ​ |  359-371 ​nm  |  395 nm  |  > 397 nm  | 
-|  2  |  Green BP  |  [[https://​www.micro-shop.zeiss.com/?​s=163176628fcf844&​l=en&​p=us&​f=f&​a=v&​b=f&​id=488010-9901-000&​o=|FS10]] ​ |  450-490 nm  |  515-565 nm  | +|  2  |  Green BP  |  [[https://​www.micro-shop.zeiss.com/?​s=163176628fcf844&​l=en&​p=us&​f=f&​a=v&​b=f&​id=488010-9901-000&​o=|FS10]] ​ |  450-490 ​nm  |  510 nm  |  515-565 nm  | 
-|  3  |  Red  |  [[https://​www.micro-shop.zeiss.com/?​s=163176628fcf844&​l=en&​p=us&​f=f&​a=v&​b=f&​id=488020-9901-000&​o=|FS20]] ​ |  540-552 nm  |  575-640 nm  | +|  3  |  Red  |  [[https://​www.micro-shop.zeiss.com/?​s=163176628fcf844&​l=en&​p=us&​f=f&​a=v&​b=f&​id=488020-9901-000&​o=|FS20]] ​ |  540-552 ​nm  |  560 nm  |  575-640 nm  | 
-|  4  |  Far Red  |  [[http://​www.semrock.com/​SetDetails.aspx?​id=2983|Cy5-4040C-000]] ​ |  608-648 nm  |  672-712 nm  | +|  4  |  Far Red  |  [[http://​www.semrock.com/​SetDetails.aspx?​id=2983|Cy5-4040C-000]] ​ |  608-648 ​nm  |  660 nm  |  672-712 nm  | 
-|  5  |  A  |  none  |  ​none  ​ ​none  ​|+|  5  |  A  |  none  ||||
  
 **Upon request:** **Upon request:**
  
-^  Position ​ ^  Filterset ​ ^  Reference ​ ^  Excitation ​ ^  Emission ​ ^ +^  Position ​ ^  Filterset ​ ^  Reference ​ ^  Excitation ​ ​^ ​ Dichroic ​ ​^ ​ Emission ​ ^ 
-|  2  |  Green LP  |  [[https://​www.micro-shop.zeiss.com/?​s=163176628fcf844&​l=en&​p=us&​f=f&​a=v&​b=f&​id=488009-9901-000&​o=|FS09]] ​ |  450-490 nm  |  > 515 nm  |+|  2  |  Green LP  |  [[https://​www.micro-shop.zeiss.com/?​s=163176628fcf844&​l=en&​p=us&​f=f&​a=v&​b=f&​id=488009-9901-000&​o=|FS09]] ​ |  450-490 ​nm  |  510 nm  |  > 515 nm  |
  
 ==== Objectives ==== ==== Objectives ====
 ^  Magnification ​ ^  Model  ^  Immersion ​ ^  NA  ^  WD (mm)  ^  pixel size* (μm)  ^  10 μm =  ^ (color) ​ 10 μm =  ^  Reference ​ ^ ^  Magnification ​ ^  Model  ^  Immersion ​ ^  NA  ^  WD (mm)  ^  pixel size* (μm)  ^  10 μm =  ^ (color) ​ 10 μm =  ^  Reference ​ ^
-|  10x  |  EC Plan-Neofluar ​ |  Air  |  0.30  |  5. ​| ​ 1.00  |  10 pixels ​ |  23.3 pixels ​ |  [[https://​www.micro-shop.zeiss.com/​en/​de/​shop/​objectives/​420340-9901-000/​Objective-EC-Plan-Neofluar-10x-0.30-M27|420340-9901-000]] ​ | +|  10x  |  EC Plan-Neofluar ​ |  Air  |  0.30  |  5.20  ​| ​ 1.00  |  10 pixels ​ |  23.3 pixels ​ |  [[https://​www.micro-shop.zeiss.com/​en/​de/​shop/​objectives/​420340-9900-000/​Objective-EC-Plan-Neofluar-10x-0.30-M27|420340-9900-000]] ​ | 
-|  20x  |  EC Plan-Neofluar ​ |  Air  |  0.50  |  2  |  0.48  |  21 pixels ​ |  46.7 pixels ​ |  [[https://​www.micro-shop.zeiss.com/​en/​de/​shop/​objectives/​420350-9900-000/​Objective-EC-Plan-Neofluar-20x-0.50-M27|420350-9900-000]] ​ |+|  20x  |  EC Plan-Neofluar ​ |  Air  |  0.50  |  2.00  ​| ​ 0.48  |  21 pixels ​ |  46.7 pixels ​ |  [[https://​www.micro-shop.zeiss.com/​en/​de/​shop/​objectives/​440340-9904-000/​Objective-EC-Plan-Neofluar-20x-0.5|440340-9904-000]] ​ |
 |  40x  |  EC Plan-NeoFluar ​ |  Air  |  0.75  |  0.71  |  0.24  |  41 pixels ​ |  93.7 pixels ​ |  [[https://​www.micro-shop.zeiss.com/​en/​de/​shop/​objectives/​440350-9903-000/​Objective-EC-Plan-Neofluar-40x-0.75|440350-9903-000]] ​ | |  40x  |  EC Plan-NeoFluar ​ |  Air  |  0.75  |  0.71  |  0.24  |  41 pixels ​ |  93.7 pixels ​ |  [[https://​www.micro-shop.zeiss.com/​en/​de/​shop/​objectives/​440350-9903-000/​Objective-EC-Plan-Neofluar-40x-0.75|440350-9903-000]] ​ |
 |  63x  |  Plan-Apochromat DIC  |  Oil  |  1.40  |  0.19  |  0.15  |  65 pixels ​ |  -  |  [[https://​www.micro-shop.zeiss.com/​en/​de/​shop/​objectives/​420782-9900-000/​Objective-Plan-Apochromat-63x-1.4-Oil-DIC-M27|420782-9900-000]] ​ | |  63x  |  Plan-Apochromat DIC  |  Oil  |  1.40  |  0.19  |  0.15  |  65 pixels ​ |  -  |  [[https://​www.micro-shop.zeiss.com/​en/​de/​shop/​objectives/​420782-9900-000/​Objective-Plan-Apochromat-63x-1.4-Oil-DIC-M27|420782-9900-000]] ​ |
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 |  1.25x  |  EC Epiplan-Neofluar ​ |  Air  |  0.035  |  4  |  7.58  |  1.3 pixels ​ |  [[https://​www.micro-shop.zeiss.com/​en/​de/​shop/​objectives/​422310-9900-000/​Objective-EC-Epiplan-Neofluar-1.25x-0.03-M27|422310-9900-000]] ​ | |  1.25x  |  EC Epiplan-Neofluar ​ |  Air  |  0.035  |  4  |  7.58  |  1.3 pixels ​ |  [[https://​www.micro-shop.zeiss.com/​en/​de/​shop/​objectives/​422310-9900-000/​Objective-EC-Epiplan-Neofluar-1.25x-0.03-M27|422310-9900-000]] ​ |
 |  5x  |  EC Plan-NeoFluar ​ |  Air  |  0.16  |  18.5  |  2.00  |  5 pixels ​ |  [[https://​www.micro-shop.zeiss.com/​en/​de/​shop/​objectives/​420330-9901-000/​Objective-EC-Plan-Neofluar-5x-0.16-M27|420330-9901-000]] ​ | |  5x  |  EC Plan-NeoFluar ​ |  Air  |  0.16  |  18.5  |  2.00  |  5 pixels ​ |  [[https://​www.micro-shop.zeiss.com/​en/​de/​shop/​objectives/​420330-9901-000/​Objective-EC-Plan-Neofluar-5x-0.16-M27|420330-9901-000]] ​ |
-|  20x  |  EC Plan-Neofluar Ph 2  |  Air  |  0.50  |  2  |  0.48  |  21 pixels ​ |  [[https://​www.micro-shop.zeiss.com/​en/​de/​shop/​objectives/​440341-9904-000/​Objective-EC-Plan-Neofluar-20x-0.5-Ph2|440341-9904-000]] ​ | 
 |  40x  |  Plan-Neofluar Ph3  |  Oil  |  1.30  |  0.20  |  0.24  |  41 pixels ​ |  [[https://​www.micro-shop.zeiss.com/​en/​de/​shop/​objectives/​440451-9903-000/​Objective-EC-Plan-Neofluar-40x-1.30-Oil-Ph3|440451-9903-000]] ​ | |  40x  |  Plan-Neofluar Ph3  |  Oil  |  1.30  |  0.20  |  0.24  |  41 pixels ​ |  [[https://​www.micro-shop.zeiss.com/​en/​de/​shop/​objectives/​440451-9903-000/​Objective-EC-Plan-Neofluar-40x-1.30-Oil-Ph3|440451-9903-000]] ​ |
 |  63x  |  Plan-Apochromat Ph3  |  Oil  |  1.4  |  0.19  |  0.15  |  65 pixels ​ |  [[https://​www.micro-shop.zeiss.com/​en/​de/​shop/​objectives/​420781-9910-000/​Objective-Plan-Apochromat-63-1.4-Oil-Ph3-M27|420781-9910-000]] ​ | |  63x  |  Plan-Apochromat Ph3  |  Oil  |  1.4  |  0.19  |  0.15  |  65 pixels ​ |  [[https://​www.micro-shop.zeiss.com/​en/​de/​shop/​objectives/​420781-9910-000/​Objective-Plan-Apochromat-63-1.4-Oil-Ph3-M27|420781-9910-000]] ​ |
 +|  100x  |  α Plan-Apochromat DIC  |  Oil  |  1.46  |  0.11  |  0.09  |  105 pixels ​ |  [[https://​www.micro-shop.zeiss.com/​en/​de/​shop/​objectives/​420792-9800-000/​Objective-alpha-Plan-Apochromat-100x-1.46-Oil-DIC-M27|420792-9800-000]] ​ |
  
 *** pixel sizes for binning = 1** *** pixel sizes for binning = 1**
zeiss_axiovert_200m.1579559980.txt.gz · Last modified: 2020/01/20 23:39 by bioimaging