User Tools

Site Tools


zeiss_axiovert_200m

Differences

This shows you the differences between two versions of the page.

Link to this comparison view

Both sides previous revision Previous revision
Next revision
Previous revision
Next revision Both sides next revision
zeiss_axiovert_200m [2017/07/13 16:27]
bioimaging
zeiss_axiovert_200m [2018/09/21 16:30]
bioimaging [Booking Rules]
Line 30: Line 30:
 ===== Microscope overview ===== ===== Microscope overview =====
  
-The Zeiss Axiovert 200M is a fully motorized inverted //widefield fluorescence microscope//​ ideal for live-cell imaging applications. It is equipped with a small stage incubator for temperature control and CO2 supply. Using the Metamorph software you can control the motorized filters, shutters and stage to simultaneously set up multi-color time-lapse imaging experiments in multiple stage positions. Its sensitive cooled CCD camera (Roper Scientific ​Coolsnap HQ CCD) allows you to capture weak fluorescent signals and minimize photobleaching/​photodamage in light sensitive samples. You can also perform Z-stack acquisition and use our [[http://​micro.magnet.fsu.edu/​primer/​digitalimaging/​deconvolution/​deconvolutionhome.html|deconvolution]] server [[brainstorm|Brainstorm]] to significantly improve image resolution even in thick samples. If you are performing long time-lapse experiments in live samples (aqueous media) and need hardware focus control, check the [[zeiss_cell_observer|Zeiss Cell Observer]] with Definite Focus. If don't want to use deconvolution,​ check out one of the confocal systems such as the [[zeiss_lsm_710|Zeiss LSM 710]][[zeiss_lsm_880|Zeiss LSM 880]] or the [[3i_marianas_sdc|3i Marianas SDC]] spinning disk. If your personal computer cannot handle all the data you collected, check out the [[big_guy|Big Guy]] or [[colossus|Colossus]]. If you have FISH or other fixed fluorescence slides try the [[leica_dm5000b|Leica DM5000B]] instead.+The Zeiss Axiovert 200M is a fully motorized inverted //widefield fluorescence microscope//​ ideal for live-cell imaging applications. It is equipped with a small stage incubator for temperature control and CO2 supply. Using the Metamorph software you can control the motorized filters, shutters and stage to simultaneously set up multi-color time-lapse imaging experiments in multiple stage positions. Its sensitive cooled CCD camera (Photometrics ​Coolsnap HQ CCD) allows you to capture weak fluorescent signals and minimize photobleaching/​photodamage in light sensitive samples. You can also perform Z-stack acquisition and use our [[http://​micro.magnet.fsu.edu/​primer/​digitalimaging/​deconvolution/​deconvolutionhome.html|deconvolution]] server [[brainstorm|Brainstorm]] to significantly improve image resolution even in thick samples. If you are performing long time-lapse experiments in live samples (aqueous media) and need hardware focus control, check the [[zeiss_cell_observer|Zeiss Cell Observer]] with Definite Focus. If don't want to use deconvolution,​ check out one of the confocal systems such as the point scanners ​[[zeiss_lsm_710|Zeiss LSM 710]] and [[zeiss_lsm_880|Zeiss LSM 880]] or the spinning disks [[3i_marianas_sdc|3i Marianas SDC]] and [[zeiss_cell_observer_sd|Zeiss Cell Observer SD]]. If your personal computer cannot handle all the data you collected, check out the [[big_guy|Big Guy]] or [[colossus|Colossus]]. If you have FISH or other fixed fluorescence slides try the [[leica_dm5000b|Leica DM5000B]] instead
 + 
 +  * **Microscope**:​ Zeiss Axiovert 200 
 +  * **Camera**: Photometrics Coolsnap HQ CCD 
 + 
 +{{warning.jpg?​20|}} Data files older than **1 month** will be automatically deleted on this system, please copy your data to the iMM server using the desktop link.
  
 ===== Booking Rules ===== ===== Booking Rules =====
Line 41: Line 46:
   - The above rules do not apply for sessions booked on the very same day or **24 hours ahead**   - The above rules do not apply for sessions booked on the very same day or **24 hours ahead**
   - Exceptions to these rules require **approval from [[joserino@medicina.ulisboa.pt|José Rino]]**.   - Exceptions to these rules require **approval from [[joserino@medicina.ulisboa.pt|José Rino]]**.
-  - Users must indicate if they will use the incubator in the booking Description field (write down 37C INCUBATOR) 
  
 ===== System components ===== ===== System components =====
Line 94: Line 98:
 {{ax_cam_on.jpg?​100|}} {{ax_cam_on.jpg?​100|}}
   * Turn the computer on.   * Turn the computer on.
-  * Login to the computer (each group has it's own area and login).+  * Login to the computer (Axiovert 200M user).
   * Start the Metamorph software.   * Start the Metamorph software.
   * If you need temperature control, turn on the control box   * If you need temperature control, turn on the control box
zeiss_axiovert_200m.txt · Last modified: 2024/02/12 22:50 by bioimaging