zeiss_axio_observer
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zeiss_axio_observer [2016/06/29 11:08] bioimaging [Booking Rules] |
zeiss_axio_observer [2016/09/27 15:30] bioimaging [Objectives] |
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| - The above rules do not apply for sessions booked on the very same day or **24 hours ahead** | - The above rules do not apply for sessions booked on the very same day or **24 hours ahead** | ||
| - Exceptions to these rules require **approval from [[joserino@medicina.ulisboa.pt|José Rino]]**. | - Exceptions to these rules require **approval from [[joserino@medicina.ulisboa.pt|José Rino]]**. | ||
| - | - Users must indicate which lasers they are going to use in the booking Description field | ||
| - Users must indicate if they will use the incubator in the booking Description field (write down 37C INCUBATOR) | - Users must indicate if they will use the incubator in the booking Description field (write down 37C INCUBATOR) | ||
| - | - Users must write their internal extension phone number in the booking Description field | ||
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| ===== System components ===== | ===== System components ===== | ||
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| | 40x | [[https://www.micro-shop.zeiss.com/?s=3407786617d462&l=en&p=uk&f=o&a=v&m=s&id=440451-9903-000|Plan-Neofluar Ph3]] | Oil | 1.30 | 0.20 | | | 40x | [[https://www.micro-shop.zeiss.com/?s=3407786617d462&l=en&p=uk&f=o&a=v&m=s&id=440451-9903-000|Plan-Neofluar Ph3]] | Oil | 1.30 | 0.20 | | ||
| | 63x | [[https://www.micro-shop.zeiss.com/?s=571858182d6d9d&l=en&p=uk&f=o&a=v&m=s&id=420781-9910-000|Plan-Apochromat Ph3]] | Oil | 1.4 | 0.19 | | | 63x | [[https://www.micro-shop.zeiss.com/?s=571858182d6d9d&l=en&p=uk&f=o&a=v&m=s&id=420781-9910-000|Plan-Apochromat Ph3]] | Oil | 1.4 | 0.19 | | ||
| - | | 100x | [[https://www.micro-shop.zeiss.com/?s=7138707e1a1de&l=en&p=uk&f=o&a=v&m=s&id=420792-9800-000|alpha Plan-Apochromat]] | Oil | 1.46 | 0.11 | | + | | 100x | [[https://www.micro-shop.zeiss.com/?s=3407786617d462&l=en&p=uk&f=o&a=v&m=s&id=440782-9902-000|Plan-Apochromat]] | Oil | 1.40 | 0.17 | |
| ===== System Turn On Procedures ===== | ===== System Turn On Procedures ===== | ||
| * Turn on the HXP 120 fluorescence light. | * Turn on the HXP 120 fluorescence light. | ||
| - | {{zeiss_cell_observer_wf_hxp.jpg?120|}} | + | {{zeiss_axio_observer_hxp.jpg?120|}} |
| * Turn on the Temperature and CO2 controller (if you need them). | * Turn on the Temperature and CO2 controller (if you need them). | ||
| - | {{zeiss_cell_observer_wf_temp.jpg?80|}} | + | {{zeiss_axio_observer_temp.jpg?80|}} |
| * If you need CO2, open the valve | * If you need CO2, open the valve | ||
| - | {{zeiss_cell_observer_wf_co2.jpg?80|}} | + | {{zeiss_axio_observer_co2.jpg?80|}} |
| * Turn on the Motorized Stage controller (A) | * Turn on the Motorized Stage controller (A) | ||
| - | {{zeiss_cell_observer_wf_a.jpg?height=150|}} | + | {{zeiss_axio_observer_a.jpg?height=150|}} |
| * Turn on the Definite Focus controller (if you are going to use it) | * Turn on the Definite Focus controller (if you are going to use it) | ||
| - | {{zeiss_cell_observer_wf_df.jpg?height=150|}} | + | {{zeiss_axio_observer_df.jpg?height=150|}} |
| * Turn on the Microscope power supply (B) | * Turn on the Microscope power supply (B) | ||
| - | {{zeiss_cell_observer_wf_b.jpg?height=150|}} | + | {{zeiss_axio_observer_b.jpg?height=150|}} |
| * Turn on the computer | * Turn on the computer | ||
| * Log in Windows with your lab user account (each lab has it's own area and login). | * Log in Windows with your lab user account (each lab has it's own area and login). | ||
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