zeiss_axio_observer
Differences
This shows you the differences between two versions of the page.
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zeiss_axio_observer [2016/06/29 11:05] bioimaging created |
zeiss_axio_observer [2017/04/13 18:31] bioimaging removed |
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| - | → <a href="https://imm.medicina.ulisboa.pt/intranet/booking/125"><img src="/facility/bioimaging/lib/exe/fetch.php?media=date.png"> Zeiss Cell Observer WF Booking</a> | + | → <a href="https://imm.medicina.ulisboa.pt/intranet/booking/125"><img src="/facility/bioimaging/lib/exe/fetch.php?media=date.png"> Zeiss Axio Observer Booking</a> |
| <br> | <br> | ||
| - | → <a href="/facility/bioimaging/doku.php?id=zeiss_cell_observer_wf_quality"><img src="/facility/bioimaging/lib/exe/fetch.php?media=list-icon.png" width=18> Zeiss Cell Observer WF Quality Control</a> | + | → <a href="/facility/bioimaging/doku.php?id=zeiss_axio_observer_quality"><img src="/facility/bioimaging/lib/exe/fetch.php?media=list-icon.png" width=18> Zeiss Axio Observer Quality Control</a> |
| <br> | <br> | ||
| - | → <a href="/facility/bioimaging/doku.php?id=zeiss_cell_observer_wf_usage"><img src="/facility/bioimaging/lib/exe/fetch.php?media=chart_line.png"> Zeiss Cell Observer WF Usage Statistics</a> | + | → <a href="/facility/bioimaging/doku.php?id=zeiss_axio_observer_usage"><img src="/facility/bioimaging/lib/exe/fetch.php?media=chart_line.png"> Zeiss Axio Observer Usage Statistics</a> |
| </p> | </p> | ||
| </td> | </td> | ||
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| ===== Microscope overview ===== | ===== Microscope overview ===== | ||
| - | The Zeiss Cell Observer WF is a fully motorized inverted //widefield fluorescence microscope// ideal for live-cell imaging applications. It is equipped with a large cage incubator and a small stage incubator for temperature control and CO2 supply and with Definite Focus for hardware focus control during long time-lapse acquisitions. Using the ZEN 2 software you can control the motorized filters, shutters and stage to simultaneously set up multi-color time-lapse imaging experiments in multiple stage positions. Its sensitive cooled Axiocam 506m camera allows you to capture weak fluorescent signals and minimize photobleaching/photodamage in light sensitive samples. You can also perform Z-stack acquisition and use our [[http://micro.magnet.fsu.edu/primer/digitalimaging/deconvolution/deconvolutionhome.html|deconvolution]] server [[brainstorm|Brainstorm]] to significantly improve image resolution even in thick samples. If don't want to use deconvolution, check out one of the confocal systems such as the [[zeiss_lsm_710|Zeiss LSM 710]], the [[zeiss_lsm_880|Zeiss LSM 880]] or the [[3i_marianas_sdc|3i Marianas SDC]] spinning disk. If your personal computer cannot handle all the data you collected, check out the [[big_guy|Big Guy]] or [[colossus|Colossus]]. If you have FISH or other fixed fluorescence slides try the [[leica_dm5000b|Leica DM5000B]] instead. | + | The Zeiss Axio Observer is a fully motorized inverted //widefield fluorescence microscope// ideal for live-cell imaging applications. It is equipped with a large cage incubator and a small stage incubator for temperature control and CO2 supply and with Definite Focus for hardware focus control during long time-lapse acquisitions. Using the ZEN 2 software you can control the motorized filters, shutters and stage to simultaneously set up multi-color time-lapse imaging experiments in multiple stage positions. Its sensitive cooled Axiocam 506m camera allows you to capture weak fluorescent signals and minimize photobleaching/photodamage in light sensitive samples. You can also perform Z-stack acquisition and use our [[http://micro.magnet.fsu.edu/primer/digitalimaging/deconvolution/deconvolutionhome.html|deconvolution]] server [[brainstorm|Brainstorm]] to significantly improve image resolution even in thick samples. If don't want to use deconvolution, check out one of the confocal systems such as the [[zeiss_lsm_710|Zeiss LSM 710]], the [[zeiss_lsm_880|Zeiss LSM 880]] or the [[3i_marianas_sdc|3i Marianas SDC]] spinning disk. If your personal computer cannot handle all the data you collected, check out the [[big_guy|Big Guy]] or [[colossus|Colossus]]. If you have FISH or other fixed fluorescence slides try the [[leica_dm5000b|Leica DM5000B]] instead. |
| ===== Booking Rules ===== | ===== Booking Rules ===== | ||
| - | The Zeiss Cell Observer WF booking is divided in three daily slots: | + | The Zeiss Axio Observer booking is divided in three daily slots: |
| | 9:00 to 13:00 | 13:00 to 17:00 | 17:00 to 21:00 | | | 9:00 to 13:00 | 13:00 to 17:00 | 17:00 to 21:00 | | ||
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| - The above rules do not apply for sessions booked on the very same day or **24 hours ahead** | - The above rules do not apply for sessions booked on the very same day or **24 hours ahead** | ||
| - Exceptions to these rules require **approval from [[joserino@medicina.ulisboa.pt|José Rino]]**. | - Exceptions to these rules require **approval from [[joserino@medicina.ulisboa.pt|José Rino]]**. | ||
| - | - Users must indicate which lasers they are going to use in the booking Description field | ||
| - Users must indicate if they will use the incubator in the booking Description field (write down 37C INCUBATOR) | - Users must indicate if they will use the incubator in the booking Description field (write down 37C INCUBATOR) | ||
| - | - Users must write their internal extension phone number in the booking Description field | ||
| - | |||
| ===== System components ===== | ===== System components ===== | ||
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| | 40x | [[https://www.micro-shop.zeiss.com/?s=3407786617d462&l=en&p=uk&f=o&a=v&m=s&id=440451-9903-000|Plan-Neofluar Ph3]] | Oil | 1.30 | 0.20 | | | 40x | [[https://www.micro-shop.zeiss.com/?s=3407786617d462&l=en&p=uk&f=o&a=v&m=s&id=440451-9903-000|Plan-Neofluar Ph3]] | Oil | 1.30 | 0.20 | | ||
| | 63x | [[https://www.micro-shop.zeiss.com/?s=571858182d6d9d&l=en&p=uk&f=o&a=v&m=s&id=420781-9910-000|Plan-Apochromat Ph3]] | Oil | 1.4 | 0.19 | | | 63x | [[https://www.micro-shop.zeiss.com/?s=571858182d6d9d&l=en&p=uk&f=o&a=v&m=s&id=420781-9910-000|Plan-Apochromat Ph3]] | Oil | 1.4 | 0.19 | | ||
| - | | 100x | [[https://www.micro-shop.zeiss.com/?s=7138707e1a1de&l=en&p=uk&f=o&a=v&m=s&id=420792-9800-000|alpha Plan-Apochromat]] | Oil | 1.46 | 0.11 | | + | | 100x | [[https://www.micro-shop.zeiss.com/?s=3407786617d462&l=en&p=uk&f=o&a=v&m=s&id=440782-9902-000|Plan-Apochromat]] | Oil | 1.40 | 0.17 | |
| ===== System Turn On Procedures ===== | ===== System Turn On Procedures ===== | ||
| * Turn on the HXP 120 fluorescence light. | * Turn on the HXP 120 fluorescence light. | ||
| - | {{zeiss_cell_observer_wf_hxp.jpg?120|}} | + | {{zeiss_axio_observer_hxp.jpg?120|}} |
| * Turn on the Temperature and CO2 controller (if you need them). | * Turn on the Temperature and CO2 controller (if you need them). | ||
| - | {{zeiss_cell_observer_wf_temp.jpg?80|}} | + | {{zeiss_axio_observer_temp.jpg?80|}} |
| * If you need CO2, open the valve | * If you need CO2, open the valve | ||
| - | {{zeiss_cell_observer_wf_co2.jpg?80|}} | + | {{zeiss_axio_observer_co2.jpg?80|}} |
| * Turn on the Motorized Stage controller (A) | * Turn on the Motorized Stage controller (A) | ||
| - | {{zeiss_cell_observer_wf_a.jpg?height=150|}} | + | {{zeiss_axio_observer_a.jpg?height=150|}} |
| * Turn on the Definite Focus controller (if you are going to use it) | * Turn on the Definite Focus controller (if you are going to use it) | ||
| - | {{zeiss_cell_observer_wf_df.jpg?height=150|}} | + | {{zeiss_axio_observer_df.jpg?height=150|}} |
| * Turn on the Microscope power supply (B) | * Turn on the Microscope power supply (B) | ||
| - | {{zeiss_cell_observer_wf_b.jpg?height=150|}} | + | {{zeiss_axio_observer_b.jpg?height=150|}} |
| * Turn on the computer | * Turn on the computer | ||
| * Log in Windows with your lab user account (each lab has it's own area and login). | * Log in Windows with your lab user account (each lab has it's own area and login). | ||
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