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quality [2020/03/01 23:16]
bioimaging
quality [2020/04/22 01:15] (current)
bioimaging
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 ====== Microscope Quality Control ====== ====== Microscope Quality Control ======
  
-{{::​psfj_fit.png?​150 ​ |}} **PSFs** \\+{{  ::​psfj_fit.png?​150|}} 
 +{{::​imm_question.jpg?​140 ​ |Image by Maria Henriques, João Ferreira and José Rino}} **PSFs** \\
 We image sub-resolution fluorescent beads (0.17 μm diameter) and use [[http://​www.knoplab.de/​psfj|PSFj]] to analyze the acquired Point Spread Functions (PSFs) and determine the resolution in x, y and z for each objective.\\ We image sub-resolution fluorescent beads (0.17 μm diameter) and use [[http://​www.knoplab.de/​psfj|PSFj]] to analyze the acquired Point Spread Functions (PSFs) and determine the resolution in x, y and z for each objective.\\
 \\ \\
 **Illumination**\\ **Illumination**\\
 We measure the intensity of fluorescent lamps and laser lines in each system. We measure the intensity of fluorescent lamps and laser lines in each system.
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 ==== Check the Systems ==== ==== Check the Systems ====
quality.txt · Last modified: 2020/04/22 01:15 by bioimaging