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quality [2020/03/01 23:16]
bioimaging
quality [2024/01/28 15:07] (current)
bioimaging
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-====== Microscope Quality Control ======+{{:​quality_control_neg.png|}}
  
-{{::​psfj_fit.png?​150 ​ |}} **PSFs** \\ +{{  ::​psfj_fit.png?​150|}} 
-We image sub-resolution fluorescent beads (0.17 μm diameter) and use [[http://www.knoplab.de/​psfj|PSFj]] to analyze the acquired Point Spread Functions (PSFs) and determine the resolution in x, y and z for each objective.\\+{{::​imm_question.jpg?​140 ​ |Image by Maria Henriques, João Ferreira and José Rino}} **PSFs** \\ 
 +We image sub-resolution fluorescent beads (0.17 μm diameter) and use [[https://biii.eu/​psfj|PSFj]] to analyze the acquired Point Spread Functions (PSFs) and determine the resolution in x, y and z for each objective.\\
 \\ \\
 **Illumination**\\ **Illumination**\\
-We measure the intensity of fluorescent lamps and laser lines in each system.+We measure the intensity of laser lines, LEDs and fluorescent lamps for each system. 
 +\\ 
 +\\
 \\ \\
-==== Check the Systems ==== 
  
 === Spinning Disc Confocal Microscopes === === Spinning Disc Confocal Microscopes ===
-  * [[3i_Marianas SDC_quality|3i Marianas SDC]] + 
-  * [[zeiss_cell_observer_sd_quality|Zeiss Cell Observer SD]]+[[3i_marianas_sdc_quality|{{::​3i_marianas_sdc_with_name.png?​160|}}]] [[zeiss_cell_observer_sd_quality|{{::​zeiss_cell_observer_sd_with_name.png?​160|}}]]
  
 === Point Scanning Confocal Microscopes === === Point Scanning Confocal Microscopes ===
-  * [[zeiss_lsm_880_quality|Zeiss LSM 880]] + 
-  * [[zeiss_lsm_710_quality|Zeiss LSM 710]]+[[zeiss_lsm_980_quality|{{::​zeiss_lsm_980_with_name.png?​160|}}]] [[zeiss_lsm_880_quality|{{::​zeiss_lsm_880_with_name.png?​160|}}]]
  
 === Multi-photon Microscope === === Multi-photon Microscope ===
-  * [[leica_sp8_mp_quality|Leica SP8 MP]]+ 
 +[[leica_sp8_mp_quality|{{::​leica_sp8_mp_with_name.png?​160|}}]]
  
 === Widefield Fluorescence Microscopes === === Widefield Fluorescence Microscopes ===
-  * [[zeiss_cell_observer_quality|Zeiss Cell Observer]] + 
-  * [[zeiss_axiovert200m_quality|Zeiss Axiovert 200M]]+[[zeiss_cell_observer_quality|{{::​zeiss_cell_observer_with_name.png?​160|}}]] [[zeiss_axiovert200m_quality|{{::​zeiss_axiovert_200m_with_name.png?​160|}}]] [[nikon_eclipse_ti_quality|{{::​nikon_eclipse_ti_with_name.png?​160|}}]] [[leica_dm5000b_quality|{{::​leica_dm5000b_with_name.png?​160|}}]] 
 + 
 +=== Fluorescence Stereo Microscope === 
 + 
 +[[zeiss_axiozoom_v16_quality|{{::​zeiss_axiozoom_v16_with_name.png?​160|}}]] 
 + 
 +==== Other Imaging Systems ==== 
 + 
 +[[ivis_lumina_quality|{{::​ivis_lumina_with_name.png?​160|}}]] 
  
 ---- ----
 === No longer available... === === No longer available... ===
  
 +  * [[zeiss_lsm_710_quality|Zeiss LSM 710]] (retired)
   * [[zeiss_lsm_7_live_quality|Zeiss LSM 7 Live]] (retired)   * [[zeiss_lsm_7_live_quality|Zeiss LSM 7 Live]] (retired)
   * [[Zeiss_LSM 510 META_quality|LSM 510 META]] (retired)   * [[Zeiss_LSM 510 META_quality|LSM 510 META]] (retired)
  
 ---- ----
-[[resources|Back to the Resources page]]+[[start|Back to the Bioimaging Wiki]]
quality.1583100998.txt.gz · Last modified: 2020/03/01 23:16 by bioimaging