PSFs
We image sub-resolution fluorescent beads (0.17 μm diameter) and use PSFj to analyze the acquired Point Spread Functions (PSFs) and determine the resolution in x, y and z for each objective.

Illumination
We measure the intensity of fluorescent lamps and laser lines in each system.

• 3i Marianas SDC
• Zeiss Cell Observer SD

• Zeiss LSM 880
• Zeiss LSM 710

• Leica SP8 MP

• Zeiss Cell Observer
• Zeiss Axiovert 200M

• Zeiss LSM 7 Live (retired)
• LSM 510 META (retired)

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