User Tools

Site Tools


quality

Differences

This shows you the differences between two versions of the page.

Link to this comparison view

Both sides previous revision Previous revision
Next revision
Previous revision
quality [2017/03/29 12:07]
bioimaging
quality [2021/04/06 00:02] (current)
bioimaging
Line 1: Line 1:
-====== Microscope Quality Control ======+{{:​quality_control_neg.png|}}
  
-==== PSFs ==== +{{  ::​psfj_fit.png?​150|}} 
- +{{::​imm_question.jpg?140  ​|Image by Maria Henriques, João Ferreira and José Rino}} **PSFs** \\ 
-{{psf.gif?100 |}} We image sub-resolution fluorescent beads (0.17 μm diameter) and use [[http://imagejdocu.tudor.lu/doku.php?​id=plugin:​analysis:​metroloj:​start|MetroloJ]] to analyze the acquired Point Spread Functions (PSFs) and determine the resolution in x, y and z for each objective. +We image sub-resolution fluorescent beads (0.17 μm diameter) and use [[http://www.knoplab.de/psfj|PSFj]] to analyze the acquired Point Spread Functions (PSFs) and determine the resolution in x, y and z for each objective.\\ 
- +\\ 
-==== Illumination ​==== +**Illumination**\\ 
- +We measure the intensity of laser lines, LEDs and fluorescent lamps for each system. 
-We measure the intensity of fluorescent lamps and laser lines in each system. +\\ 
- +\\ 
-==== Check the Systems ====+\\
  
 === Spinning Disc Confocal Microscopes === === Spinning Disc Confocal Microscopes ===
-  * [[3i_Marianas SDC_quality|3i Marianas SDC]] +  * [[3i_marianas_sdc_quality|{{:​3i_marianas_sdc.png?​150|}}]] ​[[3i_Marianas SDC_quality|3i Marianas SDC]] 
-  * [[zeiss_cell_observer_sd_quality|Zeiss Cell Observer SD]]+  * [[zeiss_cell_observer_sd_quality|{{:​zeiss_cell_observer_sd.png?​150|}}]] ​[[zeiss_cell_observer_sd_quality|Zeiss Cell Observer SD]]
  
 === Point Scanning Confocal Microscopes === === Point Scanning Confocal Microscopes ===
-  * [[zeiss_lsm_880_quality|Zeiss LSM 880]] +  * [[zeiss_lsm_880_quality|{{:​zeiss_lsm_880.png?​150|}}]] ​[[zeiss_lsm_880_quality|Zeiss LSM 880]] 
-  * [[zeiss_lsm_710_quality|Zeiss LSM 710]]+  * [[zeiss_lsm_710_quality|{{:​zeiss_lsm_710.png?​150|}}]] ​[[zeiss_lsm_710_quality|Zeiss LSM 710]]
  
 === Multi-photon Microscope === === Multi-photon Microscope ===
-  * [[leica_sp8_mp_quality|Leica SP8 MP]]+  * [[leica_sp8_mp_quality|{{:​leica_sp8_mp.png?​150|}}]] ​[[leica_sp8_mp_quality|Leica SP8 MP]]
  
 === Widefield Fluorescence Microscopes === === Widefield Fluorescence Microscopes ===
-  * [[zeiss_axio_observer_quality|Zeiss ​Axio Observer]] +  * [[zeiss_cell_observer_quality|{{::​zeiss_cell_observer.png?​150|}}]] [[zeiss_cell_observer_quality|Zeiss ​Cell Observer]] 
-  * [[zeiss_axiovert200m_quality|Zeiss Axiovert 200M]]+  * [[zeiss_axiovert_200m_quality|{{::​zeiss_axiovert_200m.png?​150|}}]] ​[[zeiss_axiovert200m_quality|Zeiss Axiovert 200M]] 
 +  * [[nikon_eclipse_ti_quality|{{::​nikon_eclipse_ti.png?​150|}}]] [[nikon_eclipse_ti_quality|Nikon Eclipse Ti]] 
 +  * [[leica_dm5000b_quality|{{::​leica_dm5000b.png?​150|}}]] [[leica_dm5000b_quality|Leica DM5000B]] 
 + 
 +==== Fluorescence Stereo Microscopes ==== 
 +  * [[zeiss_axiozoom_v16_quality|{{::​zeiss_axiozoom_v16.png?​150|}}]] [[zeiss_axiozoom_v16_quality|Zeiss AxioZoom V16]] 
 + 
 +=== Other Imaging Systems === 
 +  * [[ivis_lumina_quality|{{::​ivis_lumina.png?​150|}}]] [[ivis_lumina_quality|IVIS Lumina]]
  
 ---- ----
Line 33: Line 41:
  
 ---- ----
-[[resources|Back to the Resources page]]+[[start|Back to the Bioimaging Wiki]]
quality.1490782051.txt.gz · Last modified: 2017/03/29 12:07 by bioimaging