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quality [2016/06/29 12:50]
bioimaging
quality [2021/04/06 00:02] (current)
bioimaging
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-====== Microscope Quality Control ======+{{:​quality_control_neg.png|}}
  
-==== PSFs ====+{{  ::​psfj_fit.png?​150|}} 
 +{{::​imm_question.jpg?​140 ​ |Image by Maria Henriques, João Ferreira and José Rino}} **PSFs** \\ 
 +We image sub-resolution fluorescent beads (0.17 μm diameter) and use [[http://​www.knoplab.de/​psfj|PSFj]] to analyze the acquired Point Spread Functions (PSFs) and determine the resolution in x, y and z for each objective.\\ 
 +\\ 
 +**Illumination**\\ 
 +We measure the intensity of laser lines, LEDs and fluorescent lamps for each system. 
 +\\ 
 +\\ 
 +\\
  
-{{psf.gif?100 |}} We image sub-resolution fluorescent beads (0.17 μm diameter) and use [[http://​imagejdocu.tudor.lu/​doku.php?id=plugin:​analysis:​metroloj:​start|MetroloJ]] to analyze the acquired Point Spread Functions (PSFs) and determine the resolution in x, y and z for each objective.+=== Spinning Disc Confocal Microscopes === 
 +  * [[3i_marianas_sdc_quality|{{:​3i_marianas_sdc.png?150|}}]] [[3i_Marianas SDC_quality|3i Marianas SDC]] 
 +  * [[zeiss_cell_observer_sd_quality|{{:zeiss_cell_observer_sd.png?150|}}]] [[zeiss_cell_observer_sd_quality|Zeiss Cell Observer SD]]
  
-==== Illumination ====+=== Point Scanning Confocal Microscopes ​=== 
 +  * [[zeiss_lsm_880_quality|{{:​zeiss_lsm_880.png?​150|}}]] [[zeiss_lsm_880_quality|Zeiss LSM 880]] 
 +  * [[zeiss_lsm_710_quality|{{:​zeiss_lsm_710.png?​150|}}]] [[zeiss_lsm_710_quality|Zeiss LSM 710]]
  
-We measure the intensity of fluorescent lamps and laser lines in each system.+=== Multi-photon Microscope === 
 +  * [[leica_sp8_mp_quality|{{:​leica_sp8_mp.png?​150|}}]] [[leica_sp8_mp_quality|Leica SP8 MP]]
  
-==== Check the Systems ====+=== Widefield Fluorescence Microscopes ​=== 
 +  * [[zeiss_cell_observer_quality|{{::​zeiss_cell_observer.png?​150|}}]] [[zeiss_cell_observer_quality|Zeiss Cell Observer]] 
 +  * [[zeiss_axiovert_200m_quality|{{::​zeiss_axiovert_200m.png?​150|}}]] [[zeiss_axiovert200m_quality|Zeiss Axiovert 200M]] 
 +  * [[nikon_eclipse_ti_quality|{{::​nikon_eclipse_ti.png?​150|}}]] [[nikon_eclipse_ti_quality|Nikon Eclipse Ti]] 
 +  * [[leica_dm5000b_quality|{{::​leica_dm5000b.png?​150|}}]] [[leica_dm5000b_quality|Leica DM5000B]]
  
-=== High Speed Confocal ​Microscopes === +==== Fluorescence Stereo ​Microscopes ​==== 
-  * [[3i_Marianas SDC_quality|3i Marianas SDC]] +  * [[zeiss_axiozoom_v16_quality|{{::​zeiss_axiozoom_v16.png?​150|}}]] [[zeiss_axiozoom_v16_quality|Zeiss ​AxioZoom V16]]
-  * [[zeiss_lsm_7_live_quality|Zeiss ​LSM 7 Live]]+
  
-=== Point Scanning Confocal Microscopes ​=== +=== Other Imaging Systems ​=== 
-  * [[zeiss_lsm_880_quality|Zeiss LSM 880]] +  * [[ivis_lumina_quality|{{::​ivis_lumina.png?​150|}}]] [[ivis_lumina_quality|IVIS Lumina]]
-  * [[zeiss_lsm_710_quality|Zeiss LSM 710]] +
- +
-=== Widefield Fluorescence Microscopes === +
-  * [[zeiss_axio_observer_quality|Zeiss Axio Observer]] +
-  * [[zeiss_axiovert200m_quality|Zeiss Axiovert 200M]]+
  
 ---- ----
 === No longer available... === === No longer available... ===
  
-  * [[Zeiss_LSM 510 META_quality|LSM 510 META (retired)]]+  ​* [[zeiss_lsm_7_live_quality|Zeiss LSM 7 Live]] (retired) 
 +  ​* [[Zeiss_LSM 510 META_quality|LSM 510 META]] (retired)
  
 ---- ----
-[[resources|Back to the Resources page]]+[[start|Back to the Bioimaging Wiki]]
quality.1467197405.txt.gz · Last modified: 2016/06/29 12:50 by bioimaging