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====== Microscope Quality Control ======

{{  ::psfj_fit.png?150|}}
{{::imm_question.jpg?140  |Image by Maria Henriques, João Ferreira and José Rino}} **PSFs** \\
We image sub-resolution fluorescent beads (0.17 μm diameter) and use [[http://www.knoplab.de/psfj|PSFj]] to analyze the acquired Point Spread Functions (PSFs) and determine the resolution in x, y and z for each objective.\\
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**Illumination**\\
We measure the intensity of fluorescent lamps and laser lines in each system.
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==== Check the Systems ====

=== Spinning Disc Confocal Microscopes ===
  * [[3i_Marianas SDC_quality|3i Marianas SDC]]
  * [[zeiss_cell_observer_sd_quality|Zeiss Cell Observer SD]]

=== Point Scanning Confocal Microscopes ===
  * [[zeiss_lsm_880_quality|Zeiss LSM 880]]
  * [[zeiss_lsm_710_quality|Zeiss LSM 710]]

=== Multi-photon Microscope ===
  * [[leica_sp8_mp_quality|Leica SP8 MP]]

=== Widefield Fluorescence Microscopes ===
  * [[zeiss_cell_observer_quality|Zeiss Cell Observer]]
  * [[zeiss_axiovert200m_quality|Zeiss Axiovert 200M]]

=== Other Imaging Systems ===
  * [[ivis_lumina_quality|IVIS Lumina]]

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=== No longer available... ===

  * [[zeiss_lsm_7_live_quality|Zeiss LSM 7 Live]] (retired)
  * [[Zeiss_LSM 510 META_quality|LSM 510 META]] (retired)

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[[resources|Back to the Resources page]]

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