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quality [2022/01/31 15:52] bioimaging |
quality [2022/10/15 16:33] bioimaging |
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{{ ::psfj_fit.png?150|}} | {{ ::psfj_fit.png?150|}} | ||
{{::imm_question.jpg?140 |Image by Maria Henriques, João Ferreira and José Rino}} **PSFs** \\ | {{::imm_question.jpg?140 |Image by Maria Henriques, João Ferreira and José Rino}} **PSFs** \\ | ||
- | We image sub-resolution fluorescent beads (0.17 μm diameter) and use [[http://www.knoplab.de/psfj|PSFj]] to analyze the acquired Point Spread Functions (PSFs) and determine the resolution in x, y and z for each objective.\\ | + | We image sub-resolution fluorescent beads (0.17 μm diameter) and use [[https://github.com/cmongis/psfj|PSFj]] to analyze the acquired Point Spread Functions (PSFs) and determine the resolution in x, y and z for each objective.\\ |
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**Illumination**\\ | **Illumination**\\ | ||
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=== Point Scanning Confocal Microscopes === | === Point Scanning Confocal Microscopes === | ||
- | [[zeiss_lsm_710_quality|{{::zeiss_lsm_710_with_name.png?160|}}]] [[zeiss_lsm_880_quality|{{::zeiss_lsm_880_with_name.png?160|}}]] | + | [[zeiss_lsm_710_quality|{{::zeiss_lsm_710_with_name.png?160|}}]] [[zeiss_lsm_880_quality|{{::zeiss_lsm_880_with_name.png?160|}}]] [[zeiss_lsm_980_quality|{{::zeiss_lsm_980_with_name.png?160|}}]] |
=== Multi-photon Microscope === | === Multi-photon Microscope === |