quality
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quality [2020/03/01 23:16] bioimaging |
quality [2021/01/17 05:17] bioimaging |
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| - | ====== Microscope Quality Control ====== | + | {{:quality_control_neg.png|}} |
| - | {{::psfj_fit.png?150 |}} **PSFs** \\ | + | {{ ::psfj_fit.png?150|}} |
| + | {{::imm_question.jpg?140 |Image by Maria Henriques, João Ferreira and José Rino}} **PSFs** \\ | ||
| We image sub-resolution fluorescent beads (0.17 μm diameter) and use [[http://www.knoplab.de/psfj|PSFj]] to analyze the acquired Point Spread Functions (PSFs) and determine the resolution in x, y and z for each objective.\\ | We image sub-resolution fluorescent beads (0.17 μm diameter) and use [[http://www.knoplab.de/psfj|PSFj]] to analyze the acquired Point Spread Functions (PSFs) and determine the resolution in x, y and z for each objective.\\ | ||
| \\ | \\ | ||
| **Illumination**\\ | **Illumination**\\ | ||
| - | We measure the intensity of fluorescent lamps and laser lines in each system. | + | We measure the intensity of laser lines, LEDs and fluorescent lamps for each system. |
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| - | ==== Check the Systems ==== | ||
| === Spinning Disc Confocal Microscopes === | === Spinning Disc Confocal Microscopes === | ||
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| * [[zeiss_cell_observer_quality|Zeiss Cell Observer]] | * [[zeiss_cell_observer_quality|Zeiss Cell Observer]] | ||
| * [[zeiss_axiovert200m_quality|Zeiss Axiovert 200M]] | * [[zeiss_axiovert200m_quality|Zeiss Axiovert 200M]] | ||
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| + | === Other Imaging Systems === | ||
| + | * [[ivis_lumina_quality|IVIS Lumina]] | ||
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| - | [[resources|Back to the Resources page]] | + | [[start|Back to the Bioimaging Wiki]] |
quality.txt · Last modified: 2024/01/28 15:07 by bioimaging
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