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quality [2020/03/01 23:16] bioimaging |
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====== Microscope Quality Control ====== | ====== Microscope Quality Control ====== | ||
- | {{::psfj_fit.png?150 |}} **PSFs** \\ | + | {{ ::psfj_fit.png?150|}} |
+ | {{::imm_question.jpg?140 |Image by Maria Henriques, João Ferreira and José Rino}} **PSFs** \\ | ||
We image sub-resolution fluorescent beads (0.17 μm diameter) and use [[http://www.knoplab.de/psfj|PSFj]] to analyze the acquired Point Spread Functions (PSFs) and determine the resolution in x, y and z for each objective.\\ | We image sub-resolution fluorescent beads (0.17 μm diameter) and use [[http://www.knoplab.de/psfj|PSFj]] to analyze the acquired Point Spread Functions (PSFs) and determine the resolution in x, y and z for each objective.\\ | ||
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**Illumination**\\ | **Illumination**\\ | ||
We measure the intensity of fluorescent lamps and laser lines in each system. | We measure the intensity of fluorescent lamps and laser lines in each system. | ||
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==== Check the Systems ==== | ==== Check the Systems ==== |