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quality [2018/05/28 20:22] bioimaging |
quality [2021/01/17 04:50] bioimaging [Check the Systems] |
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====== Microscope Quality Control ====== | ====== Microscope Quality Control ====== | ||
- | ==== PSFs ==== | + | {{ ::psfj_fit.png?150|}} |
- | + | {{::imm_question.jpg?140 |Image by Maria Henriques, João Ferreira and José Rino}} **PSFs** \\ | |
- | {{psf.gif?100 |}} We image sub-resolution fluorescent beads (0.17 μm diameter) and use [[http://www.knoplab.de/psfj|PSFj]] to analyze the acquired Point Spread Functions (PSFs) and determine the resolution in x, y and z for each objective. | + | We image sub-resolution fluorescent beads (0.17 μm diameter) and use [[http://www.knoplab.de/psfj|PSFj]] to analyze the acquired Point Spread Functions (PSFs) and determine the resolution in x, y and z for each objective.\\ |
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- | ==== Illumination ==== | + | **Illumination**\\ |
We measure the intensity of fluorescent lamps and laser lines in each system. | We measure the intensity of fluorescent lamps and laser lines in each system. | ||
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==== Check the Systems ==== | ==== Check the Systems ==== | ||
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* [[zeiss_cell_observer_quality|Zeiss Cell Observer]] | * [[zeiss_cell_observer_quality|Zeiss Cell Observer]] | ||
* [[zeiss_axiovert200m_quality|Zeiss Axiovert 200M]] | * [[zeiss_axiovert200m_quality|Zeiss Axiovert 200M]] | ||
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+ | === Other Imaging Systems === | ||
+ | * [[ivis_lumina_quality|IVIS Lumina]] | ||
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- | [[resources|Back to the Resources page]] | + | [[main|Back to the Bioimaging Wiki]] |