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quality [2017/03/29 12:07] bioimaging |
quality [2020/04/22 01:15] bioimaging |
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====== Microscope Quality Control ====== | ====== Microscope Quality Control ====== | ||
- | ==== PSFs ==== | + | {{ ::psfj_fit.png?150|}} |
- | + | {{::imm_question.jpg?140 |Image by Maria Henriques, João Ferreira and José Rino}} **PSFs** \\ | |
- | {{psf.gif?100 |}} We image sub-resolution fluorescent beads (0.17 μm diameter) and use [[http://imagejdocu.tudor.lu/doku.php?id=plugin:analysis:metroloj:start|MetroloJ]] to analyze the acquired Point Spread Functions (PSFs) and determine the resolution in x, y and z for each objective. | + | We image sub-resolution fluorescent beads (0.17 μm diameter) and use [[http://www.knoplab.de/psfj|PSFj]] to analyze the acquired Point Spread Functions (PSFs) and determine the resolution in x, y and z for each objective.\\ |
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- | ==== Illumination ==== | + | **Illumination**\\ |
We measure the intensity of fluorescent lamps and laser lines in each system. | We measure the intensity of fluorescent lamps and laser lines in each system. | ||
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==== Check the Systems ==== | ==== Check the Systems ==== | ||
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=== Widefield Fluorescence Microscopes === | === Widefield Fluorescence Microscopes === | ||
- | * [[zeiss_axio_observer_quality|Zeiss Axio Observer]] | + | * [[zeiss_cell_observer_quality|Zeiss Cell Observer]] |
* [[zeiss_axiovert200m_quality|Zeiss Axiovert 200M]] | * [[zeiss_axiovert200m_quality|Zeiss Axiovert 200M]] | ||