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quality [2016/03/09 21:33] bioimaging [PSFs] |
quality [2021/04/05 23:37] bioimaging |
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- | ====== Microscope Quality Control ====== | + | {{:quality_control_neg.png|}} |
- | ==== PSFs ==== | + | {{ ::psfj_fit.png?150|}} |
+ | {{::imm_question.jpg?140 |Image by Maria Henriques, João Ferreira and José Rino}} **PSFs** \\ | ||
+ | We image sub-resolution fluorescent beads (0.17 μm diameter) and use [[http://www.knoplab.de/psfj|PSFj]] to analyze the acquired Point Spread Functions (PSFs) and determine the resolution in x, y and z for each objective.\\ | ||
+ | \\ | ||
+ | **Illumination**\\ | ||
+ | We measure the intensity of laser lines, LEDs and fluorescent lamps for each system. | ||
+ | \\ | ||
+ | \\ | ||
+ | \\ | ||
- | {{psf.gif?100 |}} We image sub-resolution fluorescent beads (0.17 μm diameter) and use [[http://imagejdocu.tudor.lu/doku.php?id=plugin:analysis:metroloj:start|MetroloJ]] to analyze the acquired Point Spread Functions (PSFs) and determine the resolution in x, y and z for each objective. | + | === Spinning Disc Confocal Microscopes === |
+ | * [[3i_marianas_sdc_quality|{{:3i_marianas_sdc.png?150|}}]] [[3i_Marianas SDC_quality|3i Marianas SDC]] | ||
+ | * [[zeiss_cell_observer_sd_quality|{{:zeiss_cell_observer_sd.png?150|}}]] [[zeiss_cell_observer_sd_quality|Zeiss Cell Observer SD]] | ||
- | ==== Illumination ==== | + | === Point Scanning Confocal Microscopes === |
+ | * [[zeiss_lsm_880_quality|{{:zeiss_lsm_880.png?150|}}]] [[zeiss_lsm_880_quality|Zeiss LSM 880]] | ||
+ | * [[zeiss_lsm_710_quality|{{:zeiss_lsm_710.png?150|}}]] [[zeiss_lsm_710_quality|Zeiss LSM 710]] | ||
- | We measure the intensity of fluorescent lamps and laser lines in each system. | + | === Multi-photon Microscope === |
+ | * [[leica_sp8_mp_quality|{{:leica_sp8_mp.png?150|}}]] [[leica_sp8_mp_quality|Leica SP8 MP]] | ||
- | ==== Check the Systems ==== | + | === Widefield Fluorescence Microscopes === |
+ | * [[zeiss_cell_observer_quality|{{::zeiss_cell_observer.png?150|}}]] [[zeiss_cell_observer_quality|Zeiss Cell Observer]] | ||
+ | * [[zeiss_axiovert_200m_quality|{{::zeiss_axiovert_200m.png?150|}}]] [[zeiss_axiovert200m_quality|Zeiss Axiovert 200M]] | ||
+ | * [[nikon_eclipse_ti_quality|{{::nikon_eclipse_ti.png?150|}}]] [[nikon_eclipse_ti_quality|Nikon Eclipse Ti]] | ||
- | === High Speed Confocal Microscopes === | + | === Other Imaging Systems === |
- | * [[3i_Marianas SDC_quality|3i Marianas SDC]] | + | * [[ivis_lumina_quality|{{::ivis_lumina.png?150|}}]] [[ivis_lumina_quality|IVIS Lumina]] |
- | * [[zeiss_lsm_7_live_quality|Zeiss LSM 7 Live]] | + | |
- | + | ||
- | === Point Scanning Confocal Microscopes === | + | |
- | * [[zeiss_lsm_880_quality|Zeiss LSM 880]] | + | |
- | * [[zeiss_lsm_710_quality|Zeiss LSM 710]] | + | |
- | + | ||
- | === Widefield Fluorescence Microscopes === | + | |
- | * [[zeiss_axiovert200m_quality|Zeiss Axiovert 200M]] | + | |
- | * [[zeiss_cell_observer_wf_quality|Zeiss Cell Observer WF]] | + | |
---- | ---- | ||
=== No longer available... === | === No longer available... === | ||
- | * [[Zeiss_LSM 510 META_quality|LSM 510 META (retired)]] | + | * [[zeiss_lsm_7_live_quality|Zeiss LSM 7 Live]] (retired) |
+ | * [[Zeiss_LSM 510 META_quality|LSM 510 META]] (retired) | ||
---- | ---- | ||
- | [[resources|Back to the Resources page]] | + | [[start|Back to the Bioimaging Wiki]] |