Bioimaging Wiki

The 3i Marianas SDC II super-resolution spinning disk confocal microscope is a fast imaging system which provides a trade-off between confocality, resolution and speed, well suited for the observation of dynamics in living cells and small organisms. The Marianas SDC provides fast acquisition (up to ~100fps, with limited ROI, ~30FPS full frame), and with lower phototoxicity and bleaching. A spinning disk with multiple small pinholes is installed between the light source and specimen to generate point-like illumination covering the whole area “simultaneously”. Each small aperture serves as a detecting pinhole to remove out-of-focus light. This system was built to be versatile, and allow imaging of different sample sizes. It includes both high and low magnification objectives (which require different pinhole sizes, 25 or 50um), DIC, TIRF, a removable heating stage with CO2 delivery, and a photomanipulation module (Phasor). The scanhead SoRa allows 1x, 2.8x and 4x magnification (for extra magnification and to allow Nyquist sampling and deconvolution).

• Microscope: Nikon Ti2 Eclipse
• Confocal scanner: Yokogawa CSU-W1 + Uniformizer
• Camera: Photometrics Prime 95B

Data files older than 1 month will be automatically deleted on this system, please copy your data to the GIMM server using the desktop link.

Back to the Equipment page