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Table of Contents
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Location: [Oeiras] Room 0B02 |
Nikon HTM-HCS Quality Control
Nikon HTM-HCS Usage StatisticsMicroscope overview
The Nikon-HTM/HCS microscope is a fully automated microscope capable of high-throughput imaging and screening of samples (eg, Ibidi chamber slides or well-plates); with the help of fully programmable and customizable acquisition protocols.
The system is equipped with a CoolLED fluorescence illuminator with 4 excitation bands, and an Andor Zyla 4.2 sCMOS camera with 4.0 MPixels with a large field of view capable of maximum of 100 FPS at full frame (2048×2048).
The optics available include fluorescence filters and brightfield, including DIC (for several magnifications) and phase-contrast (40x LWD only).
System components
| Position | Filterset | Reference | Excitation | Dichroic | Emission |
|---|---|---|---|---|---|
Objectives
| Magnification | Model | Immersion | NA | WD (mm) | PFS compatible | DIC compatible | PhC compatible | Reference |
|---|---|---|---|---|---|---|---|---|
| 10x | Plan Apo | Air | 0.45 | 4 | No | Yes (N1) | No | MRD70170 |
| 20x | Plan Apo Lambda | Air | 0.75 | 1 | Yes | Yes (N1) | No | MRD00205* |
| 100x | Plan Apo Lambda | Oil | 1.45 | 0.13 | Yes | Yes (N2) | No | MRD01905* |
| 40x | Plan Fluor | Oil | 1.3 | 0.24 | Yes | Yes (N2) | No | MRH01401 |
| 40x | Plan Apo | Air | 0.95 | 0.17-0.25 | Yes | Yes (N2) | No | MRD00400* |
| 40x | LWD* * | Air | 0.55 | 2.1 | No | No | Yes | MRP46402 |
* Not fabricated anymore ; * * LWD - Long Working Distance
Camera
