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nikon_eclipse_ti [2020/06/01 19:25]
bioimaging
nikon_eclipse_ti [2024/02/12 22:47] (current)
bioimaging
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-<a href="/​facility/​bioimaging/​lib/​exe/​fetch.php?​media=nikon_eclipse_ti_wiki_new.png"><​img src="/​facility/​bioimaging/​lib/​exe/​fetch.php?​media=nikon_eclipse_ti_wiki_new.png" width=300></​a>​+<a href="/​facility/​bioimaging/​lib/​exe/​fetch.php?​media=nikon_eclipse_ti_wiki_2022.png"><​img src="/​facility/​bioimaging/​lib/​exe/​fetch.php?​media=nikon_eclipse_ti_wiki_2022.png" width=300></​a>​
 <td style="​border:​0px solid white;">​ <p style="​line-height:​1.8">​ <td style="​border:​0px solid white;">​ <p style="​line-height:​1.8">​
-<​b>​Location</​b>:​ <a href="/​facility/​bioimaging/​lib/​exe/​fetch.php?​media=nikon_eclipse_ti_map_new.png">​Room ​P3-A-5</a> <​br> ​+<​b>​Location</​b>:​ <a href="/​facility/​bioimaging/​lib/​exe/​fetch.php?​media=nikon_eclipse_ti_map_new_p2a24.png">​Room ​P2-A-24</​a> ​(<img src="/​facility/​bioimaging/​lib/​exe/​fetch.php?​media=phone_neg.png"​ width=15>​ 47219) ​<​br> ​
 <​b>​Manufacturer</​b>:​ <a href="​https://​www.microscope.healthcare.nikon.com/​en_EU/"​ target="​_blank">​Nikon Instruments</​a><​br>​ <​b>​Manufacturer</​b>:​ <a href="​https://​www.microscope.healthcare.nikon.com/​en_EU/"​ target="​_blank">​Nikon Instruments</​a><​br>​
 <​b>​Model</​b>:​ Eclipse Ti<​br>​ <​b>​Model</​b>:​ Eclipse Ti<​br>​
 <​b>​Nickname</​b>:​ "​006"<​br>​ <​b>​Nickname</​b>:​ "​006"<​br>​
-<​b>​Software</​b>: ​Metamorph / NIS-Elements AR 5.11.01<br>+<​b>​Software</​b>:​ NIS-Elements AR 5.42.05<br>
 <​b>​Year</​b>:​ 2010 <br> <​b>​Year</​b>:​ 2010 <br>
 <​b>​SN</​b>:​ 602805 <br> <​b>​SN</​b>:​ 602805 <br>
 &nbsp <br> &nbsp <br>
 +Data will be deleted after: <b>3 months</​b>​
 &nbsp <br> &nbsp <br>
 &nbsp <br> &nbsp <br>
 &nbsp <br> &nbsp <br>
-&rarr; <a href="https://my.agendo.science/​calendar/?id=MzhjYWxlbmRhcnM="><​img src="/​facility/​bioimaging/​lib/​exe/​fetch.php?​media=date.png"> ​ Nikon Eclipse Ti Booking</a>+&rarr; <a href="/​facility/bioimaging/doku.php?id=nikon_eclipse_ti_quality"><​img src="/​facility/​bioimaging/​lib/​exe/​fetch.php?​media=list-icon.png" ​width=18> Nikon Eclipse Ti Quality Control</a>
  <​br> ​  <​br> ​
 &rarr; <a href="/​facility/​bioimaging/​doku.php?​id=nikon_eclipse_ti_usage"><​img src="/​facility/​bioimaging/​lib/​exe/​fetch.php?​media=chart_line.png"> ​ Nikon Eclipse Ti Usage Statistics</​a>​ &rarr; <a href="/​facility/​bioimaging/​doku.php?​id=nikon_eclipse_ti_usage"><​img src="/​facility/​bioimaging/​lib/​exe/​fetch.php?​media=chart_line.png"> ​ Nikon Eclipse Ti Usage Statistics</​a>​
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 ===== Microscope overview ===== ===== Microscope overview =====
-{{  :​widefield_lightpath.png?​0x300}}The Nikon Eclipse Ti is a fully motorized inverted //widefield fluorescence microscope//​ ideal for live-cell imaging applications. It is equipped with a small stage incubator for temperature control and CO2 supply and with Perfect Focus System for hardware focus control during long time-lapse acquisitions. Using either Metamorph or Nikon Elements software you can control the motorized filters, shutters and stage to simultaneously set up multi-color time-lapse imaging experiments in multiple stage positions. It is equipped with a Retiga R6 monochrome ​CCD camera ​that allows for a large field of view, ideal for time-lapse and tile-and-stitch microscopy. You can perform Z-stack acquisition and use [[https://​huygens.i3s.up.pt/​login.php|PPBI Huygens RM]] [[http://​micro.magnet.fsu.edu/​primer/​digitalimaging/​deconvolution/​deconvolutionhome.html|deconvolution]] server to significantly improve image resolution even in thick samples. If don't want to use deconvolution,​ check out one of the confocal systems such as the point scanners [[zeiss_lsm_710|Zeiss LSM 710]] and [[zeiss_lsm_880|Zeiss LSM 880]] or spinning disks [[3i_marianas_sdc|3i Marianas SDC]] and [[zeiss_cell_observer_sd|Zeiss Cell Observer SD]]. If your personal computer cannot handle all the data you collected, check out the [[big_guy|Big Guy]] or [[colossus|Colossus]].+{{  :​widefield_lightpath.png?​0x300}}The Nikon Eclipse Ti is a fully motorized inverted //widefield fluorescence microscope//​ ideal for live-cell imaging applications. It is equipped with a small stage incubator for temperature control and CO2 supplyand with Perfect Focus System for hardware focus control during long time-lapse acquisitions. Using NIS-Elements software you can control the motorized filters, shutters and stage to simultaneously set up multi-color time-lapse imaging experiments in multiple stage positions. It is equipped with an Andor Neo 5.5 monochrome ​cooled sCMOS camera. ​
  
   * **Microscope**:​ [[https://​www.microscope.healthcare.nikon.com/​en_EU/​products/​inverted-microscopes/​eclipse-ti-series|Nikon Eclipse Ti]]   * **Microscope**:​ [[https://​www.microscope.healthcare.nikon.com/​en_EU/​products/​inverted-microscopes/​eclipse-ti-series|Nikon Eclipse Ti]]
-  * **Camera**: [[https://​www.qimaging.com/retiga-r6|Teledyne QImaging Retiga R6 mono CCD]]+  * **Camera**: ​[[https://​andor.oxinst.com/​products/​scmos-camera-series/​neo-5-5-scmos|Andor Neo 5.5 sCMOS]] 
 + 
 +This microscope is also equipped with a [[https://​www.ionoptix.com/products/​systems/​calcium-and-contractility-system/​|Ionoptix Calcium and Contractility system]] for high-speed simultaneous acquisition of Fura-2 fluorescence with video-based cell length measurement to study myocyte function, contracting spontaneously or with field stimulation. 
 + 
 +{{::​ionoptix_realtime-acquisition-cell-length-600x500.jpg?​200 ​ |}} 
 +  * **High-speed contractility camera**: [[https://​www.ionoptix.com/​products/​components/​sensors/​myocam-s3/​|MyoCam-S3]] 
 +  * **Electrical Field Stimulator**:​ [[https://​www.ionoptix.com/​products/​components/​stimulators/​myopacer-cell-stimulator/​|MyoPacer]] 
 +  * **LED Light Source**: [[https://​www.ionoptix.com/​products/​components/​light-sources/​led-light-source/​|UV LEDs for Fura-2 excitation]] 
 +  * **High-speed PMT**: [[https://​www.ionoptix.com/​products/​components/​sensors/​pmt-sub-system/​|PMT400]] for photon counting at 1kHz 
 +  * **Software**:​ [[https://​www.ionoptix.com/​products/​software/​ionwizard-core-and-analysis/​|IonWizard]] 7.7.3.180
  
 {{:​warning.png?​25|}} Data files older than **3 months** will be automatically deleted on this system, please copy your data to the iMM server using the desktop link. {{:​warning.png?​25|}} Data files older than **3 months** will be automatically deleted on this system, please copy your data to the iMM server using the desktop link.
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 ===== System components ===== ===== System components =====
  
-==== Filtersets ====+==== Filtersets ​Turret 1 (top) ====
  
 ^  Position ​ ^  Filterset ​ ^  Reference ​ ^  Excitation ​ ^  Dichroic ​ ^  Emission ​ ^ ^  Position ​ ^  Filterset ​ ^  Reference ​ ^  Excitation ​ ^  Dichroic ​ ^  Emission ​ ^
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 |  5  |  Cy5  |  2SK4CY50 ​ |  608-648 nm  |  660 nm  |  672-712 nm  | |  5  |  Cy5  |  2SK4CY50 ​ |  608-648 nm  |  660 nm  |  672-712 nm  |
 |  6  |  Empty  ||||| |  6  |  Empty  |||||
 +
 +==== Filtersets Turret 2 (bottom) ====
 +
 +^  Position ​ ^  Filterset ​ ^
 +|  1  |  Empty  |
 +|  2  |  Empty  |
 +|  3  |  Empty  |
 +|  4  |  Empty  |
 +|  5  |  Empty  |
 +|  6  |  Fura-2 ​ |
  
 ==== Objectives ==== ==== Objectives ====
 ^  Magnification ​ ^  Model  ^  Immersion ​ ^  NA  ^  WD (mm)  ^  Reference ​ ^ ^  Magnification ​ ^  Model  ^  Immersion ​ ^  NA  ^  WD (mm)  ^  Reference ​ ^
-|  10x  |  CFI Plan Fluor DLL Ph1  |  Air  |  0.30  |  16.00  |  [[https://​www.microscope.healthcare.nikon.com/​en_EU/​selectors/​objective-comparison/​-1835|MRH10101]] ​ |+|  10x  |  CFI Plan Fluor DLL Ph1  |  Air  |  0.30  |  16.00  |   ​[[https://​www.microscope.healthcare.nikon.com/​en_EU/​selectors/​objective-comparison/​-1835|MRH10101]]  | 
 +|  20x  |  CFI S Plan Fluor ELWD ADM Ph1  |  Air  |  0.45  |  6.90 - 8.20  |  [[https://​www.microscope.healthcare.nikon.com/​en_EU/​selectors/​objective-comparison/​-1813|MRH48230]] ​ | 
 +|  40x  |  CFI CFI Plan Apochromat Lambda D  |  Air  |  0.95  |  0.21  |  [[https://​www.microscope.healthcare.nikon.com/​products/​optics/​selector/​comparison/​-179806|MRH70470]]  |
 |  40x  |  CFI S Plan Fluor ELWD ADM Ph2  |  Air  |  0.60  |  2.80 - 3.60  |  [[https://​www.microscope.healthcare.nikon.com/​en_EU/​selectors/​objective-comparison/​-1814|MRH48430]] ​ | |  40x  |  CFI S Plan Fluor ELWD ADM Ph2  |  Air  |  0.60  |  2.80 - 3.60  |  [[https://​www.microscope.healthcare.nikon.com/​en_EU/​selectors/​objective-comparison/​-1814|MRH48430]] ​ |
-|  ​40x  ​|  ​CFI Plan Apo DM Ph3  |  ​Water  ​| ​ 1.00  ​| ​ 0.16  ​| ​   |+|  ​60x  ​| ​ Plan Apo DM Ph3  |  ​Oil  ​| ​ 1.40  ​| ​ 0.13  ​| ​ | 
 +|  100x  |  Plan Apo DM Ph3  |  Oil  |  1.40  |  0.13  |  ​|
  
 **Upon request:** **Upon request:**
 ^  Magnification ​ ^  Model  ^  Immersion ​ ^  NA  ^  WD (mm)  ^  Reference ​ ^ ^  Magnification ​ ^  Model  ^  Immersion ​ ^  NA  ^  WD (mm)  ^  Reference ​ ^
-|  40x  |  CFI S Plan Fluor ELWD ADL Ph2  |  Air  |  0.60  |  2.70 - 3.70  |    | 
 |  100x  |  CFI Plan  |  Water  |  1.10  |  2.50  |  [[https://​www.microscope.healthcare.nikon.com/​en_EU/​selectors/​objective-comparison/​-1956|MRL07920]] ​ | |  100x  |  CFI Plan  |  Water  |  1.10  |  2.50  |  [[https://​www.microscope.healthcare.nikon.com/​en_EU/​selectors/​objective-comparison/​-1956|MRL07920]] ​ |
- 
 ==== Camera ==== ==== Camera ====
 ^  Model  ^  Type  ^  Frame Size  ^  Pixel Size (µm)  ^ ^  Model  ^  Type  ^  Frame Size  ^  Pixel Size (µm)  ^
-|  [[https://www.qimaging.com/retiga-r6|Retiga R6 mono CCD]]  |  CCD  |  ​2688 2200  ​|  ​4.54 4.54  |+|  [[https://andor.oxinst.com/products/​scmos-camera-series/​neo-5-5-scmos|Andor Neo 5.5 sCMOS]]  |  CCD  |  ​2560 2160  ​|  ​6.6. |
  
 ---- ----
-[[resources|Back to the Resources ​page]]+[[resources|Back to the Equipment ​page]]
nikon_eclipse_ti.1591032331.txt.gz · Last modified: 2020/06/01 19:25 by bioimaging