Bioimaging Wiki

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<b>Location</b>: <a href="/facility/bioimaging/lib/exe/fetch.php?media=nikon_eclipse_ti_map_new.png">Room P3-A-5</a> <br> 
<b>Manufacturer</b>: <a href="https://www.microscope.healthcare.nikon.com/en_EU/" target="_blank">Nikon Instruments</a><br>
<b>Model</b>: Eclipse Ti<br>
<b>Nickname</b>: "006"<br>
<b>Software</b>: Metamorph / NIS-Elements AR 5.11.01<br>
<b>Year</b>: 2010 <br>
<b>SN</b>: 602805 <br>
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&rarr; <a href="https://my.agendo.science/calendar/?id=MzhjYWxlbmRhcnM="><img src="/facility/bioimaging/lib/exe/fetch.php?media=date.png">  Nikon Eclipse Ti Booking</a>
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&rarr; <a href="/facility/bioimaging/doku.php?id=nikon_eclipse_ti_usage"><img src="/facility/bioimaging/lib/exe/fetch.php?media=chart_line.png">  Nikon Eclipse Ti Usage Statistics</a>
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===== Microscope overview =====
{{  :widefield_lightpath.png?0x300}}The Nikon Eclipse Ti is a fully motorized inverted //widefield fluorescence microscope// ideal for live-cell imaging applications. It is equipped with a small stage incubator for temperature control and CO2 supply and with Perfect Focus System for hardware focus control during long time-lapse acquisitions. Using either Metamorph or Nikon Elements software you can control the motorized filters, shutters and stage to simultaneously set up multi-color time-lapse imaging experiments in multiple stage positions. It is equipped with a Retiga R6 monochrome CCD camera that allows for a large field of view, ideal for time-lapse and tile-and-stitch microscopy. You can perform Z-stack acquisition and use [[https://huygens.i3s.up.pt/login.php|PPBI Huygens RM]] [[http://micro.magnet.fsu.edu/primer/digitalimaging/deconvolution/deconvolutionhome.html|deconvolution]] server to significantly improve image resolution even in thick samples. If don't want to use deconvolution, check out one of the confocal systems such as the point scanners [[zeiss_lsm_710|Zeiss LSM 710]] and [[zeiss_lsm_880|Zeiss LSM 880]] or spinning disks [[3i_marianas_sdc|3i Marianas SDC]] and [[zeiss_cell_observer_sd|Zeiss Cell Observer SD]]. If your personal computer cannot handle all the data you collected, check out the [[big_guy|Big Guy]] or [[colossus|Colossus]].

  * **Microscope**: [[https://www.microscope.healthcare.nikon.com/en_EU/products/inverted-microscopes/eclipse-ti-series|Nikon Eclipse Ti]]
  * **Camera**: [[https://www.qimaging.com/retiga-r6|Teledyne QImaging Retiga R6 mono CCD]]

{{:warning.png?25|}} Data files older than **3 months** will be automatically deleted on this system, please copy your data to the iMM server using the desktop link.

===== System components =====

==== Filtersets ====

^  Position  ^  Filterset  ^  Reference  ^  Excitation  ^  Dichroic  ^  Emission  ^
|  1  |  Empty  |||||
|  2  |  DAPI  |  MBE41300  |  340-380 nm  |  400 nm  |  435-485 nm  |
|  3  |  FITC  |  MBE44720  |  465-495 nm  |  505 nm  |  515-555 nm  |
|  4  |  TRITC  |  MBE45600  |  528-553 nm  |  565 nm  |  590-650 nm  |
|  5  |  Cy5  |  2SK4CY50  |  608-648 nm  |  660 nm  |  672-712 nm  |
|  6  |  Empty  |||||

==== Objectives ====
^  Magnification  ^  Model  ^  Immersion  ^  NA  ^  WD (mm)  ^  pixel size* (μm)  ^  10 μm =  ^  Reference  ^
|  10x  |  CFI Plan Fluor DLL Ph1  |  Air  |  0.30  |  16.00  |  0.454  |  22 pixels  |   [[https://www.microscope.healthcare.nikon.com/en_EU/selectors/objective-comparison/-1835|MRH10101]]  |
|  20x  |  CFI S Plan Fluor ELWD ADM Ph1  |  Air  |  0.45  |  6.90 - 8.20  |  0.227  |  44 pixels  |  [[https://www.microscope.healthcare.nikon.com/en_EU/selectors/objective-comparison/-1813|MRH48230]]  |
|  40x  |  CFI S Plan Fluor ELWD ADM Ph2  |  Air  |  0.60  |  2.80 - 3.60  |  0.115  |  86 pixels  |  [[https://www.microscope.healthcare.nikon.com/en_EU/selectors/objective-comparison/-1814|MRH48430]]  |
|  40x  |  Plan Apo DM Ph3  |  Oil  |  1.00  |  0.16  |   0.113  |  88 pixels  |  |

**Upon request:**
^  Magnification  ^  Model  ^  Immersion  ^  NA  ^  WD (mm)  ^  pixel size* (μm)  ^  10 μm =  ^  Reference  ^
|  40x  |  Plan Fluor ELWD ADL Ph2  |  Air  |  0.60  |  2.70 - 3.70  |  0.113  |  88 pixels  |  |


*** pixel sizes for binning = 1**
==== Camera ====
^  Model  ^  Type  ^  Frame Size  ^  Pixel Size (µm)  ^
|  [[https://www.qimaging.com/retiga-r6|Retiga R6 mono CCD]]  |  CCD  |  2688 x 2200  |  4.54 x 4.54  |

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