nikon_eclipse_ti
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nikon_eclipse_ti [2020/03/01 18:05] bioimaging [Filtersets] |
nikon_eclipse_ti [2024/02/12 20:40] bioimaging [Microscope overview] |
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| - | <a href="/facility/bioimaging/lib/exe/fetch.php?media=nikon_eclipse_ti_wiki_new.png"><img src="/facility/bioimaging/lib/exe/fetch.php?media=nikon_eclipse_ti_wiki_new.png" width=300></a> | + | <a href="/facility/bioimaging/lib/exe/fetch.php?media=nikon_eclipse_ti_wiki_2022.png"><img src="/facility/bioimaging/lib/exe/fetch.php?media=nikon_eclipse_ti_wiki_2022.png" width=300></a> |
| <td style="border:0px solid white;"> <p style="line-height:1.8"> | <td style="border:0px solid white;"> <p style="line-height:1.8"> | ||
| - | <b>Location</b>: <a href="/facility/bioimaging/lib/exe/fetch.php?media=nikon_eclipse_ti_map_new.png">Room P3-A-5</a> <br> | + | <b>Location</b>: <a href="/facility/bioimaging/lib/exe/fetch.php?media=nikon_eclipse_ti_map_new_p2a24.png">Room P2-A-24</a> (<img src="/facility/bioimaging/lib/exe/fetch.php?media=phone_neg.png" width=15> 47219) <br> |
| <b>Manufacturer</b>: <a href="https://www.microscope.healthcare.nikon.com/en_EU/" target="_blank">Nikon Instruments</a><br> | <b>Manufacturer</b>: <a href="https://www.microscope.healthcare.nikon.com/en_EU/" target="_blank">Nikon Instruments</a><br> | ||
| <b>Model</b>: Eclipse Ti<br> | <b>Model</b>: Eclipse Ti<br> | ||
| <b>Nickname</b>: "006"<br> | <b>Nickname</b>: "006"<br> | ||
| - | <b>Software</b>: Metamorph / NIS-Elements AR 5.11.01<br> | + | <b>Software</b>: NIS-Elements AR 5.11.01<br> |
| <b>Year</b>: 2010 <br> | <b>Year</b>: 2010 <br> | ||
| <b>SN</b>: 602805 <br> | <b>SN</b>: 602805 <br> | ||
|   <br> |   <br> | ||
| + | Data will be deleted after: <b>3 months</b> | ||
|   <br> |   <br> | ||
|   <br> |   <br> | ||
|   <br> |   <br> | ||
| - | → <a href="https://imm.medicina.ulisboa.pt/intranet/booking/163"><img src="/facility/bioimaging/lib/exe/fetch.php?media=date.png"> Nikon Eclipse Ti Booking</a> | + | → <a href="/facility/bioimaging/doku.php?id=nikon_eclipse_ti_quality"><img src="/facility/bioimaging/lib/exe/fetch.php?media=list-icon.png" width=18> Nikon Eclipse Ti Quality Control</a> |
| <br> | <br> | ||
| → <a href="/facility/bioimaging/doku.php?id=nikon_eclipse_ti_usage"><img src="/facility/bioimaging/lib/exe/fetch.php?media=chart_line.png"> Nikon Eclipse Ti Usage Statistics</a> | → <a href="/facility/bioimaging/doku.php?id=nikon_eclipse_ti_usage"><img src="/facility/bioimaging/lib/exe/fetch.php?media=chart_line.png"> Nikon Eclipse Ti Usage Statistics</a> | ||
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| ===== Microscope overview ===== | ===== Microscope overview ===== | ||
| - | {{ :widefield_lightpath.png?0x300}}The Nikon Eclipse Ti is a fully motorized inverted //widefield fluorescence microscope// ideal for live-cell imaging applications. It is equipped with a small stage incubator for temperature control and CO2 supply and with Perfect Focus System for hardware focus control during long time-lapse acquisitions. Using either Metamorph or Nikon Elements software you can control the motorized filters, shutters and stage to simultaneously set up multi-color time-lapse imaging experiments in multiple stage positions. It is equipped with a Retiga R6 monochrome CCD camera that allows for a large field of view, ideal for time-lapse and tile-and-stitch microscopy. You can perform Z-stack acquisition and use [[https://huygens.i3s.up.pt/login.php|PPBI Huygens RM]] [[http://micro.magnet.fsu.edu/primer/digitalimaging/deconvolution/deconvolutionhome.html|deconvolution]] server to significantly improve image resolution even in thick samples. If don't want to use deconvolution, check out one of the confocal systems such as the point scanners [[zeiss_lsm_710|Zeiss LSM 710]] and [[zeiss_lsm_880|Zeiss LSM 880]] or spinning disks [[3i_marianas_sdc|3i Marianas SDC]] and [[zeiss_cell_observer_sd|Zeiss Cell Observer SD]]. If your personal computer cannot handle all the data you collected, check out the [[big_guy|Big Guy]] or [[colossus|Colossus]]. | + | {{ :widefield_lightpath.png?0x300}}The Nikon Eclipse Ti is a fully motorized inverted //widefield fluorescence microscope// ideal for live-cell imaging applications. It is equipped with a small stage incubator for temperature control and CO2 supply, and with Perfect Focus System for hardware focus control during long time-lapse acquisitions. Using NIS-Elements software you can control the motorized filters, shutters and stage to simultaneously set up multi-color time-lapse imaging experiments in multiple stage positions. It is equipped with an Andor Neo 5.5 monochrome cooled sCMOS camera. |
| * **Microscope**: [[https://www.microscope.healthcare.nikon.com/en_EU/products/inverted-microscopes/eclipse-ti-series|Nikon Eclipse Ti]] | * **Microscope**: [[https://www.microscope.healthcare.nikon.com/en_EU/products/inverted-microscopes/eclipse-ti-series|Nikon Eclipse Ti]] | ||
| - | * **Camera**: [[https://www.qimaging.com/retiga-r6|Teledyne QImaging Retiga R6 mono CCD]] | + | * **Camera**: [[https://andor.oxinst.com/products/scmos-camera-series/neo-5-5-scmos|Andor Neo 5.5 sCMOS]] |
| + | |||
| + | This microscope is also equipped with a [[https://www.ionoptix.com/products/systems/calcium-and-contractility-system/|Ionoptix Calcium and Contractility system]] for high-speed simultaneous acquisition of Fura-2 fluorescence with video-based cell length measurement to study myocyte function, contracting spontaneously or with field stimulation. | ||
| + | |||
| + | {{::ionoptix_realtime-acquisition-cell-length-600x500.jpg?200 |}} | ||
| + | * **High-speed contractility camera**: [[https://www.ionoptix.com/products/components/sensors/myocam-s3/|MyoCam-S3]] | ||
| + | * **Electrical Field Stimulator**: [[https://www.ionoptix.com/products/components/stimulators/myopacer-cell-stimulator/|MyoPacer]] | ||
| + | * **LED Light Source**: [[https://www.ionoptix.com/products/components/light-sources/led-light-source/|UV LEDs for Fura-2 excitation]] | ||
| + | * **High-speed PMT**: [[https://www.ionoptix.com/products/components/sensors/pmt-sub-system/|PMT400]] for photon counting at 1kHz | ||
| + | * **Software**: [[https://www.ionoptix.com/products/software/ionwizard-core-and-analysis/|IonWizard]] 7.7.3.180 | ||
| {{:warning.png?25|}} Data files older than **3 months** will be automatically deleted on this system, please copy your data to the iMM server using the desktop link. | {{:warning.png?25|}} Data files older than **3 months** will be automatically deleted on this system, please copy your data to the iMM server using the desktop link. | ||
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| ===== System components ===== | ===== System components ===== | ||
| - | ==== Filtersets ==== | + | ==== Filtersets Turret 1 (top) ==== |
| ^ Position ^ Filterset ^ Reference ^ Excitation ^ Dichroic ^ Emission ^ | ^ Position ^ Filterset ^ Reference ^ Excitation ^ Dichroic ^ Emission ^ | ||
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| | 5 | Cy5 | 2SK4CY50 | 608-648 nm | 660 nm | 672-712 nm | | | 5 | Cy5 | 2SK4CY50 | 608-648 nm | 660 nm | 672-712 nm | | ||
| | 6 | Empty ||||| | | 6 | Empty ||||| | ||
| + | |||
| + | ==== Filtersets Turret 2 (bottom) ==== | ||
| + | |||
| + | ^ Position ^ Filterset ^ | ||
| + | | 1 | Empty | | ||
| + | | 2 | Empty | | ||
| + | | 3 | Empty | | ||
| + | | 4 | Empty | | ||
| + | | 5 | Empty | | ||
| + | | 6 | Fura-2 | | ||
| ==== Objectives ==== | ==== Objectives ==== | ||
| ^ Magnification ^ Model ^ Immersion ^ NA ^ WD (mm) ^ Reference ^ | ^ Magnification ^ Model ^ Immersion ^ NA ^ WD (mm) ^ Reference ^ | ||
| - | | 10x | CFI Plan Fluor DLL Ph1 | Air | 0.30 | 16.00 | [[https://www.microscope.healthcare.nikon.com/en_EU/selectors/objective-comparison/-1835|MRH10101]] | | + | | 10x | CFI Plan Fluor DLL Ph1 | Air | 0.30 | 16.00 | [[https://www.microscope.healthcare.nikon.com/en_EU/selectors/objective-comparison/-1835|MRH10101]] | |
| + | | 20x | CFI S Plan Fluor ELWD ADM Ph1 | Air | 0.45 | 6.90 - 8.20 | [[https://www.microscope.healthcare.nikon.com/en_EU/selectors/objective-comparison/-1813|MRH48230]] | | ||
| + | | 40x | CFI CFI Plan Apochromat Lambda D | Air | 0.95 | 0.21 | [[https://www.microscope.healthcare.nikon.com/products/optics/selector/comparison/-179806|MRH70470]] | | ||
| | 40x | CFI S Plan Fluor ELWD ADM Ph2 | Air | 0.60 | 2.80 - 3.60 | [[https://www.microscope.healthcare.nikon.com/en_EU/selectors/objective-comparison/-1814|MRH48430]] | | | 40x | CFI S Plan Fluor ELWD ADM Ph2 | Air | 0.60 | 2.80 - 3.60 | [[https://www.microscope.healthcare.nikon.com/en_EU/selectors/objective-comparison/-1814|MRH48430]] | | ||
| - | | 40x | CFI Plan Apo DM Ph3 | Water | 1.00 | 0.16 | | | + | | 60x | Plan Apo DM Ph3 | Oil | 1.40 | 0.13 | | |
| + | | 100x | Plan Apo DM Ph3 | Oil | 1.40 | 0.13 | | | ||
| **Upon request:** | **Upon request:** | ||
| ^ Magnification ^ Model ^ Immersion ^ NA ^ WD (mm) ^ Reference ^ | ^ Magnification ^ Model ^ Immersion ^ NA ^ WD (mm) ^ Reference ^ | ||
| - | | 40x | CFI S Plan Fluor ELWD ADL Ph2 | Air | 0.60 | 2.70 - 3.70 | | | ||
| | 100x | CFI Plan | Water | 1.10 | 2.50 | [[https://www.microscope.healthcare.nikon.com/en_EU/selectors/objective-comparison/-1956|MRL07920]] | | | 100x | CFI Plan | Water | 1.10 | 2.50 | [[https://www.microscope.healthcare.nikon.com/en_EU/selectors/objective-comparison/-1956|MRL07920]] | | ||
| - | |||
| ==== Camera ==== | ==== Camera ==== | ||
| ^ Model ^ Type ^ Frame Size ^ Pixel Size (µm) ^ | ^ Model ^ Type ^ Frame Size ^ Pixel Size (µm) ^ | ||
| - | | [[https://www.qimaging.com/retiga-r6|Retiga R6 mono CCD]] | CCD | 2688 x 2200 | 4.54 x 4.54 | | + | | [[https://andor.oxinst.com/products/scmos-camera-series/neo-5-5-scmos|Andor Neo 5.5 sCMOS]] | CCD | 2560 x 2160 | 6.5 x 6.5 | |
| ---- | ---- | ||
| - | [[resources|Back to the Resources page]] | + | [[resources|Back to the Equipment page]] |
nikon_eclipse_ti.txt · Last modified: 2024/02/12 22:47 by bioimaging
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