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nikon_eclipse_te2000e [2019/12/31 02:52]
bioimaging created
nikon_eclipse_te2000e [2023/08/06 13:30] (current)
bioimaging
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 <a href="/​facility/​bioimaging/​lib/​exe/​fetch.php?​media=nikon_eclipse_te2000_e_wiki_new.png"><​img src="/​facility/​bioimaging/​lib/​exe/​fetch.php?​media=nikon_eclipse_te2000_e_wiki_new.png"​ width=300></​a>​ <a href="/​facility/​bioimaging/​lib/​exe/​fetch.php?​media=nikon_eclipse_te2000_e_wiki_new.png"><​img src="/​facility/​bioimaging/​lib/​exe/​fetch.php?​media=nikon_eclipse_te2000_e_wiki_new.png"​ width=300></​a>​
 <td style="​border:​0px solid white;">​ <p style="​line-height:​1.8">​ <td style="​border:​0px solid white;">​ <p style="​line-height:​1.8">​
-<​b>​Location</​b>: ​Room P3-A-5 ​<​br> ​+<​b>​Location</​b>: ​Lab 19 (floor 0) </a><​br> ​
 <​b>​Manufacturer</​b>:​ <a href="​https://​www.microscope.healthcare.nikon.com/​en_EU/"​ target="​_blank">​Nikon Instruments</​a><​br>​ <​b>​Manufacturer</​b>:​ <a href="​https://​www.microscope.healthcare.nikon.com/​en_EU/"​ target="​_blank">​Nikon Instruments</​a><​br>​
 <​b>​Model</​b>:​ Eclipse TE2000-E<​br>​ <​b>​Model</​b>:​ Eclipse TE2000-E<​br>​
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 <​b>​Software</​b>:​ Metamorph / NIS-Elements<​br>​ <​b>​Software</​b>:​ Metamorph / NIS-Elements<​br>​
 <​b>​Year</​b>:​ 2007 <br> <​b>​Year</​b>:​ 2007 <br>
-<​b>​SN</​b>:​ <br>+<​b>​SN</​b>: ​596701<br>
 &nbsp <br> &nbsp <br>
 &nbsp <br> &nbsp <br>
 &nbsp <br> &nbsp <br>
 &nbsp <br> &nbsp <br>
-&rarr; <a href="​https://​imm.medicina.ulisboa.pt/​intranet/booking/164"><​img src="/​facility/​bioimaging/​lib/​exe/​fetch.php?​media=date.png"> ​ Nikon Eclipse TE2000-E Booking</​a>​+&rarr; <a href="​https://​my.agendo.science/calendar/?​id=MzljYWxlbmRhcnM="><​img src="/​facility/​bioimaging/​lib/​exe/​fetch.php?​media=date.png"> ​ Nikon Eclipse TE2000-E Booking</​a>​
  <​br> ​  <​br> ​
-&rarr; <a href="/​facility/​bioimaging/​doku.php?​id=nikon_eclipse_te2000_e_usage"><​img src="/​facility/​bioimaging/​lib/​exe/​fetch.php?​media=chart_line.png"> ​ Nikon Eclipse TE2000-E Usage Statistics</​a>​+&rarr; <a href="/​facility/​bioimaging/​doku.php?​id=nikon_eclipse_te2000e_usage"><​img src="/​facility/​bioimaging/​lib/​exe/​fetch.php?​media=chart_line.png"> ​ Nikon Eclipse TE2000-E Usage Statistics</​a>​
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 </​html>​ </​html>​
 +
 +===== Microscope overview =====
 +{{  :​widefield_lightpath.png?​0x300}}The Nikon Eclipse TE2000-E is a fully motorized inverted //widefield fluorescence microscope//​ ideal for live-cell imaging applications. It is equipped with a small stage incubator for temperature control and CO2 supply and with Perfect Focus System for hardware focus control during long time-lapse acquisitions. Using either Metamorph or Nikon Elements software you can control the motorized filters, shutters and stage to simultaneously set up multi-color time-lapse imaging experiments in multiple stage positions. It is equipped with a CoolSNAP EZ cooled monochrome CCD camera for fast, high-resolution imaging. You can perform Z-stack acquisition and use [[https://​huygens.i3s.up.pt/​login.php|PPBI Huygens RM]] [[http://​micro.magnet.fsu.edu/​primer/​digitalimaging/​deconvolution/​deconvolutionhome.html|deconvolution]] server to significantly improve image resolution even in thick samples. If don't want to use deconvolution,​ check out one of the confocal systems such as the point scanners [[zeiss_lsm_710|Zeiss LSM 710]] and [[zeiss_lsm_880|Zeiss LSM 880]] or spinning disks [[3i_marianas_sdc|3i Marianas SDC]] and [[zeiss_cell_observer_sd|Zeiss Cell Observer SD]]. If your personal computer cannot handle all the data you collected, check out the [[big_guy|Big Guy]] or [[colossus|Colossus]].
 +
 +  * **Microscope**:​ [[https://​www.microscopyu.com/​museum/​eclipse-te2000-inverted-microscope|Nikon Eclipse TE2000-E]]
 +  * **Camera**: [[https://​www.photometrics.com/​wp-content/​uploads/​2019/​10/​CoolSNAP-EZ-Datasheet.pdf|Photometrics CoolSNAP EZ mono CCD]]
 +
 +{{:​warning.png?​25|}} Data files older than **3 months** will be automatically deleted on this system, please copy your data to the iMM server using the desktop link.
 +
 +===== System components =====
 +
 +==== Filtersets ====
 +
 +^  Position ​ ^  Filterset ​ ^  Reference ​ ^  Excitation ​ ^  Dichroic ​ ^  Emission ​ ^
 +|  1  |  DAPI  |  MBE41300 ​ |  340-380 nm  |  400 nm  |  435-485 nm  |
 +|  2  |  TRITC  |  MBE45600 ​ |  528-553 nm  |  565 nm  |  577-632 nm  |
 +|  3  |  AF  |  none  ||||
 +|  4  |  Empty  |||||
 +|  5  |  GFP  |  MBE44720 ​ |  465-495 nm  |  505 nm  |  515-555 nm  |
 +|  6  |  Cy5  |  2SK4CY50 ​ |  608-648 nm  |  660 nm  |  672-712 nm  |
 +
 +==== Objectives ====
 +^  Magnification ​ ^  Model  ^  Immersion ​ ^  NA  ^  WD (mm)  ^  Reference ​ ^
 +|  10x  |  CFI Plan Fluor DLL Ph1  |  Air  |  0.30  |  16.00  |  [[https://​www.microscope.healthcare.nikon.com/​en_EU/​selectors/​objective-comparison/​-1835|MRH10101]] ​ |
 +|  40x  |  CFI Plan Fluor DLL Ph2  |  Air  |  0.75  |  0.66  |  [[https://​www.microscope.healthcare.nikon.com/​en_EU/​selectors/​objective-comparison/​-1838|MRH10401]] ​ |
 +|  60x  |  CFI S Plan Apo DM Ph3  |  Oil  |  1.40  |  0.13  |  [[https://​www.microscope.healthcare.nikon.com/​en_EU/​selectors/​objective-comparison/​-1876|MRD31605]] ​ |
 +
 +**Upon request:**
 +^  Magnification ​ ^  Model  ^  Immersion ​ ^  NA  ^  WD (mm)  ^  Reference ​ ^
 +|  40x  |  CFI S Plan Fluor ELWD ADL Ph 2  |  Air  |  0.60  |  2.70 - 3.70  |    |
 +|  100x  |  CFI Plan  |  Water  |  1.10  |  2.50  |  [[https://​www.microscope.healthcare.nikon.com/​en_EU/​selectors/​objective-comparison/​-1956|MRL07920]] ​ |
 +
 +==== Camera ====
 +^  Model  ^  Type  ^  Frame Size  ^  Pixel Size (µm)  ^
 +|  [[https://​www.photometrics.com/​wp-content/​uploads/​2019/​10/​CoolSNAP-EZ-Datasheet.pdf|CoolSNAP EZ mono CCD]]  |  CCD  |  1392 x 1040  |  6.45 x 6.45  |
  
 ---- ----
-[[resources|Back to the Resources ​page]]+[[resources|Back to the Equipment ​page]]
nikon_eclipse_te2000e.1577757177.txt.gz · Last modified: 2019/12/31 02:52 by bioimaging