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--- nikon_eclipse_te2000e [2019/12/31 02:54]
bioimaging
+++ nikon_eclipse_te2000e [2023/08/06 13:29]
bioimaging
@@ Line 6: / Line 6: @@
 <a href="/facility/bioimaging/lib/exe/fetch.php?media=nikon_eclipse_te2000_e_wiki_new.png"><img src="/facility/bioimaging/lib/exe/fetch.php?media=nikon_eclipse_te2000_e_wiki_new.png" width=300></a>
 <td style="border:0px solid white;"> <p style="line-height:1.8">
-<b>Location</b>: Room P3-A-5 <br>
+<b>Location</b>: retired </a><br>
 <b>Manufacturer</b>: <a href="https://www.microscope.healthcare.nikon.com/en_EU/" target="_blank">Nikon Instruments</a><br>
 <b>Model</b>: Eclipse TE2000-E<br>
@@ Line 12: / Line 12: @@
 <b>Software</b>: Metamorph / NIS-Elements<br>
 <b>Year</b>: 2007 <br>
-<b>SN</b>: <br>
+<b>SN</b>: 596701<br>
 &nbsp <br>
 &nbsp <br>
 &nbsp <br>
 &nbsp <br>
-&rarr; <a href="https://imm.medicina.ulisboa.pt/intranet/booking/164"><img src="/facility/bioimaging/lib/exe/fetch.php?media=date.png">  Nikon Eclipse TE2000-E Booking</a>
+&rarr; <a href="https://my.agendo.science/calendar/?id=MzljYWxlbmRhcnM="><img src="/facility/bioimaging/lib/exe/fetch.php?media=date.png">  Nikon Eclipse TE2000-E Booking</a>
  <br>
 &rarr; <a href="/facility/bioimaging/doku.php?id=nikon_eclipse_te2000e_usage"><img src="/facility/bioimaging/lib/exe/fetch.php?media=chart_line.png">  Nikon Eclipse TE2000-E Usage Statistics</a>
@@ Line 25: / Line 25: @@
 </table>
 </html>
+===== Microscope overview =====
+{{  :widefield_lightpath.png?0x300}}The Nikon Eclipse TE2000-E is a fully motorized inverted //widefield fluorescence microscope// ideal for live-cell imaging applications. It is equipped with a small stage incubator for temperature control and CO2 supply and with Perfect Focus System for hardware focus control during long time-lapse acquisitions. Using either Metamorph or Nikon Elements software you can control the motorized filters, shutters and stage to simultaneously set up multi-color time-lapse imaging experiments in multiple stage positions. It is equipped with a CoolSNAP EZ cooled monochrome CCD camera for fast, high-resolution imaging. You can perform Z-stack acquisition and use [[https://huygens.i3s.up.pt/login.php|PPBI Huygens RM]] [[http://micro.magnet.fsu.edu/primer/digitalimaging/deconvolution/deconvolutionhome.html|deconvolution]] server to significantly improve image resolution even in thick samples. If don't want to use deconvolution, check out one of the confocal systems such as the point scanners [[zeiss_lsm_710|Zeiss LSM 710]] and [[zeiss_lsm_880|Zeiss LSM 880]] or spinning disks [[3i_marianas_sdc|3i Marianas SDC]] and [[zeiss_cell_observer_sd|Zeiss Cell Observer SD]]. If your personal computer cannot handle all the data you collected, check out the [[big_guy|Big Guy]] or [[colossus|Colossus]].
+  * **Microscope**: [[https://www.microscopyu.com/museum/eclipse-te2000-inverted-microscope|Nikon Eclipse TE2000-E]]
+  * **Camera**: [[https://www.photometrics.com/wp-content/uploads/2019/10/CoolSNAP-EZ-Datasheet.pdf|Photometrics CoolSNAP EZ mono CCD]]
+{{:warning.png?25|}} Data files older than **3 months** will be automatically deleted on this system, please copy your data to the iMM server using the desktop link.
+===== System components =====
+==== Filtersets ====
+^  Position  ^  Filterset  ^  Reference  ^  Excitation  ^  Dichroic  ^  Emission  ^
+|  1  |  DAPI  |  MBE41300  |  340-380 nm  |  400 nm  |  435-485 nm  |
+|  2  |  TRITC  |  MBE45600  |  528-553 nm  |  565 nm  |  577-632 nm  |
+|  3  |  AF  |  none  ||||
+|  4  |  Empty  |||||
+|  5  |  GFP  |  MBE44720  |  465-495 nm  |  505 nm  |  515-555 nm  |
+|  6  |  Cy5  |  2SK4CY50  |  608-648 nm  |  660 nm  |  672-712 nm  |
+==== Objectives ====
+^  Magnification  ^  Model  ^  Immersion  ^  NA  ^  WD (mm)  ^  Reference  ^
+|  10x  |  CFI Plan Fluor DLL Ph1  |  Air  |  0.30  |  16.00  |  [[https://www.microscope.healthcare.nikon.com/en_EU/selectors/objective-comparison/-1835|MRH10101]]  |
+|  40x  |  CFI Plan Fluor DLL Ph2  |  Air  |  0.75  |  0.66  |  [[https://www.microscope.healthcare.nikon.com/en_EU/selectors/objective-comparison/-1838|MRH10401]]  |
+|  60x  |  CFI S Plan Apo DM Ph3  |  Oil  |  1.40  |  0.13  |  [[https://www.microscope.healthcare.nikon.com/en_EU/selectors/objective-comparison/-1876|MRD31605]]  |
+**Upon request:**
+^  Magnification  ^  Model  ^  Immersion  ^  NA  ^  WD (mm)  ^  Reference  ^
+|  40x  |  CFI S Plan Fluor ELWD ADL Ph 2  |  Air  |  0.60  |  2.70 - 3.70  |    |
+|  100x  |  CFI Plan  |  Water  |  1.10  |  2.50  |  [[https://www.microscope.healthcare.nikon.com/en_EU/selectors/objective-comparison/-1956|MRL07920]]  |
+==== Camera ====
+^  Model  ^  Type  ^  Frame Size  ^  Pixel Size (µm)  ^
+|  [[https://www.photometrics.com/wp-content/uploads/2019/10/CoolSNAP-EZ-Datasheet.pdf|CoolSNAP EZ mono CCD]]  |  CCD  |  1392 x 1040  |  6.45 x 6.45  |
 ----
-[[resources|Back to the Resources page]]
+[[resources|Back to the Equipment page]]

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