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 ====== BioImaging NanoCourse on Microscopy, 4 - 6 Nov 2015 ====== ====== BioImaging NanoCourse on Microscopy, 4 - 6 Nov 2015 ======
  
-{{microscope_explained.jpg?200|}}+{{nanocourse.png?200|}}
  
-“If motorists drove their cars the way many cell biologists used their confocal microscopesour highways would be littered ​with twisted wrecks of automobiles ​and the mangled corpses of run-over pedestriansEveryone recognizes that driving a car is a tricky procedure(…) ​the fact is that a confocal ​microscope is probably at least as complicated ​to operate as a car.“ +The Bioimaging Nano Course is an introductory course on Microscopy for beginners or intermediate microscope users. In this courseyou will become familiarized ​with both basic microscopy techniques ​and more advanced topics such as 3D microscopy, live imaging, FRAP, Photoactivation and FRET, during three afternoon sessionsWe will discuss all the relevant background in fluorescent widefield and confocal ​microscopy and we will also provide practical information on how to efficiently prepare samplesacquire data and obtain publication quality images.
-- Wallace Marshall“Through a pinhole, darkly”+
  
-You probably know already that you need a “driver’s license” to operate each microscope at IMM. Training each user to become fully independent and a “safe driver” is our main goal at the BioImaging Unit. As in driving schools, we sit by your side and teach you what the buttons do, when to go ahead and when to brake, ultimately how to avoid crashing into a bleached sample or even worse, into the sample itself. Pressing buttons is of course only half of what you need to know. The other half is knowing what is happening inside the microscope when you press that button. Did you ever wonder what a pinhole really is? Do you want to know how to choose filters and dichroics instead of just re-using some “magical” settings? Do you want to know what happens to light inside a widefield and a confocal, and which one of them you really need? Then this NanoCourse is for you. You will become familiar with both basic microscopy techniques and more advanced 3D microscopy and live imaging during two intensive afternoon sessions. Whether you are a beginning or intermediate microscope user, you will have the chance to learn all the relevant background in fluorescent widefield and confocal microscopy as well as obtain practical information on how to efficiently prepare samples, acquire data and obtain publication quality images.+===== Program =====
  
-===== The Program =====+=== Wednesday, November 4 - Room 57 ===
  
-=== Thursday, May 15th Room 52 ===+^  Time  ^  Topic  ^  Speaker ​ ^ 
 +|  14:00-14:30  |  Principles of Light Interaction with Matter ​ |  José Rino   | 
 +|  14:​30-15:​00 ​ |  Image Formation: Anatomy of an Optical Microscope ​ |  José Rino  | 
 +|  15:​00-15:​30 ​ |  __Break__ ​ | 
 +|  15:​30-16:​00 ​ |  Contrast Techniques in Light Microscopy ​ |  José Rino  | 
 +|  16:​00-16:​30 ​ |  Fluorescence Microscopy ​ |  José Rino  |
  
-^  Time  ^  Topic  ^ +=== Thursday, November 5 Room 52 ===
-|  14:00-14:30  |  Principles of Light Interaction with Matter ​ | +
-|  14:​30-15:​00 ​ |  Image Formation: Anatomy of an Optical Microscope ​ | +
-|  15:​00-15:​30 ​ |  Contrast Techniques in Light Microscopy ​ | +
-|  15:​30-16:​00 ​ |  __Coffee Break__ ​ | +
-|  16:​00-16:​30 ​ |  Fluorescence Microscopy ​ | +
-|  16:​30-17:​00 ​ |  Fluorescent Probes and Sample Preparation ​ | +
-|  17:​00-17:​30 ​ |  Digital Image Acquisition ​ |+
  
-=== Friday, May 16th Room 52 ===+^  Time  ^  Topic  ^  Speaker ​ ^ 
 +|  14:00-14:30  |  Fluorescent Probes and Sample Preparation ​ |  António Temudo ​ | 
 +|  14:​30-15:​00 ​ |  Image Deconvolution ​ |  José Rino  | 
 +|  15:​00-15:​30 ​ |  __Break__ ​ | 
 +|  15:​30-16:​30 ​ |  Introduction to Confocal Microscopy ​ |  José Rino  |
  
-^  Time  ^  Topic  ^ +=== Friday, November 6 - Room 52 === 
-|  14:​00-14:​30 ​ |  ​Fluorescent Proteins ​and Live Imaging ​ | + 
-|  14:30-15:30  ​|  ​Introduction to Confocal Microscopy ​ +^  Time  ^  Topic  ​^ ​ Speaker ​ ^ 
-|  ​15:​30-16:​00 ​ |  Image Deconvolution ​ | +|  14:​00-14:​30 ​ |  ​FRAP and Photoactivation ​ |  José Rino  | 
-|  ​16:00-16:30  |  ​__Coffee and snacks__ ​ | +|  14:30-15:00  ​|  ​Colocalization and FRET  ​|  ​José Rino  | 
-|  ​16:30-17:00  |  ​FRAP, FRET and Photoactivation ​ | +|  ​15:00-15:30  |  ​__Break__ ​ | 
-|  ​17:00-17:30  |  Image Processing and Analysis ​ | +|  ​15:30-16:00  |  ​The Digital Microscope ​ |  José Rino  | 
-|  ​17:30-18:00  |  Systems Overview ​ |+|  ​16:00-16:30  |  Image Processing and Analysis ​ ​| ​ José Rino  | 
 +|  ​16:30-17:00  |  Systems Overview ​and Wrap-up ​ ​| ​ José Rino  | 
 + 
 +===== Talks ===== 
 + 
 +<​html><​a href="/​facility/​bioimaging/​lib/​exe/​fetch.php?​media=01%20-%20Principles%20of%20Light%20Interaction%20with%20Matter.zip"><​img src="/​facility/​bioimaging/​lib/​exe/​fetch.php?​media=01_-_principles_of_light_interaction_with_matter.png"​ width=290></​a>​ &nbsp; </​html>​ 
 +<​html><​a href="/​facility/​bioimaging/​lib/​exe/​fetch.php?​media=02%20-%20Anatomy%20of%20an%20Optical%20Microscope.zip"><​img src="/​facility/​bioimaging/​lib/​exe/​fetch.php?​media=02_-_anatomy_of_an_optical_microscope.png"​ width=290></​a>​ &nbsp; </​html>​ 
 +<​html><​a href="/​facility/​bioimaging/​lib/​exe/​fetch.php?​media=03%20-%20Contrast%20Techniques%20in%20Optical%20Microscopy.zip"><​img src="/​facility/​bioimaging/​lib/​exe/​fetch.php?​media=03_-_contrast_techniques_in_optical_microscopy.png"​ width=290></​a>​ &nbsp; </​html>​ 
 +<​html><​BR></​html>​ 
 +<​html><​BR></​html>​ 
 +<​html><​a href="/​facility/​bioimaging/​lib/​exe/​fetch.php?​media=04%20-%20Fluorescence%20Microscopy.zip"><​img src="/​facility/​bioimaging/​lib/​exe/​fetch.php?​media=04_-_fluorescence_microscopy.png"​ width=290></​a>​ &nbsp; </​html>​ 
 +<​html><​a href="/​facility/​bioimaging/​lib/​exe/​fetch.php?​media=05%20-%20Fluorescent%20Probes%20and%20Immunofluorescence.zip"><​img src="/​facility/​bioimaging/​lib/​exe/​fetch.php?​media=05_-_fluorescent_probes_and_immunofluorescence.png"​ width=290></​a>​ &nbsp; </​html>​ 
 +<​html><​a href="/​facility/​bioimaging/​lib/​exe/​fetch.php?​media=06%20-%20Fluorescent%20Proteins%20and%20Live%20Imaging.zip"><​img src="/​facility/​bioimaging/​lib/​exe/​fetch.php?​media=06_-_fluorescent_proteins_and_live_imaging.png"​ width=290></​a>​ &nbsp; </​html>​ 
 +<​html><​BR></​html>​ 
 +<​html><​BR></​html>​ 
 +<​html><​a href="/​facility/​bioimaging/​lib/​exe/​fetch.php?​media=07%20-%20Image%20Deconvolution.zip"><​img src="/​facility/​bioimaging/​lib/​exe/​fetch.php?​media=07_-_image_deconvolution.png"​ width=290></​a>​ &nbsp; </​html>​ 
 +<​html>​ 
 +<​html><​a href="/​facility/​bioimaging/​lib/​exe/​fetch.php?​media=08%20-%20Introduction%20to%20Confocal%20Microscopy.zip"><​img src="/​facility/​bioimaging/​lib/​exe/​fetch.php?​media=08_-_introduction_to_confocal_microscopy.png"​ width=290></​a>​ &nbsp; </​html>​ 
 +<​html><​a href="/​facility/​bioimaging/​lib/​exe/​fetch.php?​media=09%20-%20FRAP%20and%20Photoactivation.zip"><​img src="/​facility/​bioimaging/​lib/​exe/​fetch.php?​media=09_-_frap_and_photoactivation.png"​ width=290></​a>​ &nbsp; </​html>​ 
 +<​html><​BR></​html>​ 
 +<​html><​BR></​html>​ 
 +<​html><​a href="/​facility/​bioimaging/​lib/​exe/​fetch.php?​media=10%20-%20Colocalization%20and%20FRET.zip"><​img src="/​facility/​bioimaging/​lib/​exe/​fetch.php?​media=10_-_colocalization_and_fret.png"​ width=290></​a>​ &nbsp; </​html>​ 
 +<​html><​a href="/​facility/​bioimaging/​lib/​exe/​fetch.php?​media=11%20-%20Digital%20Image%20Acquisition.zip"><​img src="/​facility/​bioimaging/​lib/​exe/​fetch.php?​media=11_-_digital_image_acquisition.png"​ width=290></​a>​ &nbsp; </​html>​ 
 +<​html><​a href="/​facility/​bioimaging/​lib/​exe/​fetch.php?​media=12%20-%20Image%20Processing%20and%20Analysis.zip"><​img src="/​facility/​bioimaging/​lib/​exe/​fetch.php?​media=12_-_image_processing_and_analysis.png"​ width=290></​a>​ &nbsp; </​html>​ 
 +<​html><​BR></​html>​ 
 +<​html><​BR></​html>​ 
 +<​html><​a href="/​facility/​bioimaging/​lib/​exe/​fetch.php?​media=13%20-%20Systems%20Overview.zip"><​img src="/​facility/​bioimaging/​lib/​exe/​fetch.php?​media=13_-_systems_overview.png"​ width=290></​a>​ &nbsp; </​html>​ 
 +<​html><​BR></​html>​
  
 ===== Where ===== ===== Where =====
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 ---- ----
-[[courses|Back to the Bioimaging ​Past Events]]+[[courses|Back to the Bioimaging ​Courses]]
nanocourse_2015.1564735677.txt.gz · Last modified: 2019/08/02 10:47 by bioimaging