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leica_dm5000b

Location: Room P0-B-32
Manufacturer: Leica Microsystems
Model: DM5000 B
Nickname: "5000B"
Software: Leica DC Twain
Year: 2006
SN: 269835
 
 
 
 
Leica DM5000B Booking
Leica DM5000B Usage Statistics

Microscope overview

The Leica DM5000B is an automated upright widefield fluorescence microscope ideal for imaging FISH or other fixed fluorescence slides. The microscope is prepared for brightfield, phase contrast, DIC and polarization contrast techniques (check objectives list for contrast method availability). It has a monochrome CCD camera for fluorescence image acquisition. If you want to image live samples and/or perform time-lapse experiments check out the Zeiss Axiovert 200M or the Zeiss Cell Observer instead. If you need a color camera for brightfield images, try the Leica DM2500.

Data files older than 3 months will be automatically deleted on this system, please copy your data to the iMM server using the desktop link.

System components

Filtersets

Filterset Reference Excitation Dichroic Emission Fluorochromes
Blue A4 340-380 nm 400 nm 450-490 nm DAPI, Hoechst, Alexa350
Green GFP 450-490 nm 500 nm 500-550 nm GFP, FITC, Alexa488
Red N2.1 515-560 nm 580 nm > 590 nm Rhodamine, Cy3, Texas Red

Objectives

Magnification Model Immersion NA WD (mm) pixel size* (μm) 10 μm = BF PH DIC POL Reference
5x HC PL FLUOTAR Air 0.15 12.00 2.00 5 pixels 506504
10x HC PL FLUOTAR Air 0.30 11.00 1.00 10 pixels 506507
20x HC PL FLUOTAR Air 0.50 1.15 0.53 19 pixels 506506
20x HC PLAN APO Air 0.70 0.59 0.53 19 pixels 506166
40x HCX PL FLUOTAR Air 0.75 0.40 0.26 39 pixels 506145
63x HCX PL APO Oil 1.4-0.60 0.10 0.16 63 pixels 506187

* pixel size for 1300 x 1030 images


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leica_dm5000b.txt · Last modified: 2020/06/01 19:26 by bioimaging