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leica_dm5000b [2020/03/01 11:51]
bioimaging [Objectives]
leica_dm5000b [2023/08/06 13:30] (current)
bioimaging
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-<a href="/​facility/​bioimaging/​lib/​exe/​fetch.php?​media=leica_dm5000b_wiki_new.png"><​img src="/​facility/​bioimaging/​lib/​exe/​fetch.php?​media=leica_dm5000b_wiki_new.png" width=300></​a>​+<a href="/​facility/​bioimaging/​lib/​exe/​fetch.php?​media=leica_dm5000b_wiki_2022.png"><​img src="/​facility/​bioimaging/​lib/​exe/​fetch.php?​media=leica_dm5000b_wiki_2022.png" width=300></​a>​
 <td style="​border:​0px solid white;">​ <p style="​line-height:​1.8">​ <td style="​border:​0px solid white;">​ <p style="​line-height:​1.8">​
-<​b>​Location</​b>: ​<a href="/​facility/​bioimaging/​lib/​exe/​fetch.php?​media=leica_dm5000b_map_new.png">​Room P0-B-32</​a>​<​br> ​+<​b>​Location</​b>: ​Lab 16 (floor 0)<​br> ​
 <​b>​Manufacturer</​b>:​ <a href="​http://​www.leica-microsystems.com/​home/"​ target="​_blank">​Leica Microsystems</​a><​br>​ <​b>​Manufacturer</​b>:​ <a href="​http://​www.leica-microsystems.com/​home/"​ target="​_blank">​Leica Microsystems</​a><​br>​
 <​b>​Model</​b>:​ <a href="​http://​www.leica-microsystems.com/​products/​light-microscopes/​life-science-research/​upright-microscopes/​details/​product/​leica-dm5000-b/"​ target="​_blank">​DM5000 B</​a><​br>​ <​b>​Model</​b>:​ <a href="​http://​www.leica-microsystems.com/​products/​light-microscopes/​life-science-research/​upright-microscopes/​details/​product/​leica-dm5000-b/"​ target="​_blank">​DM5000 B</​a><​br>​
 <​b>​Nickname</​b>:​ "​5000B"<​br>​ <​b>​Nickname</​b>:​ "​5000B"<​br>​
-<​b>​Software</​b>:​ Leica DC Twain<​br>​ 
 <​b>​Year</​b>:​ 2006 <br> <​b>​Year</​b>:​ 2006 <br>
 <​b>​SN</​b>:​ 269835<​br>​ <​b>​SN</​b>:​ 269835<​br>​
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 &nbsp <br> &nbsp <br>
 &nbsp <br> &nbsp <br>
-&rarr; <a href="https://imm.medicina.ulisboa.pt/intranet/​booking/​9"><​img src="/​facility/​bioimaging/​lib/​exe/​fetch.php?​media=date.png"> ​ Leica DM5000B ​Booking</​a>​ +&nbsp <​br>​ 
- <​br> ​+&rarr; <a href="/​facility/bioimaging/doku.php?​id=leica_dm5000b_quality"><​img src="/​facility/​bioimaging/​lib/​exe/​fetch.php?​media=list-icon.png" ​width=18> Leica DM5000B ​Quality Control</​a>​ 
 + <​br> ​ 
 &rarr; <a href="/​facility/​bioimaging/​doku.php?​id=leica_dm5000b_usage"><​img src="/​facility/​bioimaging/​lib/​exe/​fetch.php?​media=chart_line.png"> ​ Leica DM5000B Usage Statistics</​a>​ &rarr; <a href="/​facility/​bioimaging/​doku.php?​id=leica_dm5000b_usage"><​img src="/​facility/​bioimaging/​lib/​exe/​fetch.php?​media=chart_line.png"> ​ Leica DM5000B Usage Statistics</​a>​
 </p> </p>
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 ===== Microscope overview ===== ===== Microscope overview =====
  
-The Leica DM5000B is an automated upright //widefield fluorescence microscope//​ ideal for imaging ​FISH or other fixed fluorescence slides. The microscope is prepared for brightfield,​ phase contrast, DIC and polarization contrast techniques (check objectives list for contrast method availability). It has a monochrome CCD camera for fluorescence image acquisition. If you want to image live samples and/or perform time-lapse experiments check out the [[zeiss_axiovert_200m|Zeiss Axiovert 200M]] or the [[zeiss_cell_observer|Zeiss Cell Observer]] instead. If you need a color camera for brightfield images, try the [[leica_dm2500|Leica DM2500]]+The Leica DM5000B is an automated upright //widefield fluorescence microscope//​ ideal for checking ​FISH or other fixed fluorescence slides. The microscope is prepared for brightfield,​ phase contrast, DIC and polarization contrast techniques (check objectives list for contrast method availability). If you want to image live samples and/or perform time-lapse experiments check out the [[zeiss_axiovert_200m|Zeiss Axiovert 200M]] or the [[zeiss_cell_observer|Zeiss Cell Observer]] instead. If you need a color camera for brightfield images, try the [[leica_dm2500|Leica DM2500]].
- +
-{{:​warning.png?​25|}} Data files older than **3 months** will be automatically deleted on this system, please copy your data to the iMM server using the desktop link.+
  
 ===== System components ===== ===== System components =====
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 ==== Filtersets ==== ==== Filtersets ====
  
-^  Filterset ​ ^  Reference ​ ^  Excitation ​ ^  Emission ​ ^  Fluorochromes ​ ^ +^  Filterset ​ ^  Reference ​ ^  Excitation ​ ​^ ​ Dichroic ​ ​^ ​ Emission ​ ^  Fluorochromes ​ ^ 
-|  Blue  |  A4  |  340-380 nm        |  450-490 nm  |  DAPI, Hoechst, Alexa350 ​ | +|  Blue  |  A4  |  340-380 nm  ​| ​ 400 nm  ​|  450-490 nm  |  DAPI, Hoechst, Alexa350 ​ | 
-|  Green  |  GFP  |  450-490 nm        |  500-550 nm  |  GFP, FITC, Alexa488 ​ | +|  Green  |  GFP  |  450-490 nm  ​| ​ 500 nm  ​|  500-550 nm  |  GFP, FITC, Alexa488 ​ | 
-|  Red  |  N2.1  |  515-560 nm        |  > 590 nm  |  Rhodamine, Cy3, Texas Red  |+|  Red  |  N2.1  |  515-560 nm  ​| ​ 580 nm  ​|  > 590 nm  |  Rhodamine, Cy3, Texas Red  |
  
  
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 ==== Objectives ==== ==== Objectives ====
-^  Magnification ​ ^  Model  ^  Immersion ​ ^  NA  ^  WD (mm)  ​^  pixel size* (μm)  ^  10 μm =  ^ ^  BF  ^  PH  ^  DIC  ^  POL  ^  Reference ​ ^ +^  Magnification ​ ^  Model  ^  Immersion ​ ^  NA  ^  WD (mm)  ^  BF  ^  PH  ^  DIC  ^  POL  ^  Reference ​ ^ 
-|  5x  |  HC PL FLUOTAR ​ |  Air  |  0.15  |  12.00  ​|  2.00  |  5 pixels ​ | |  ✔  |  ✘  |  ✘  |  ✔  |  506504 ​ | +|  5x  |  HC PL FLUOTAR ​ |  Air  |  0.15  |  12.00  |  ✔  |  ✘  |  ✘  |  ✔  |  506504 ​ | 
-|  10x  |  HC PL FLUOTAR ​ |  Air  |  0.30  |  11.00  ​|  1.00  |  10 pixels ​ | |  ✔  |  ✔  |  ✘  |  ✔  |  506507 ​ | +|  10x  |  HC PL FLUOTAR ​ |  Air  |  0.30  |  11.00  |  ✔  |  ✔  |  ✘  |  ✔  |  506507 ​ | 
-|  20x  |  HC PL FLUOTAR ​ |  Air  |  0.50  |   ​1.15  ​|  0.53  |  19 pixels ​ | |  ✔  |  ✔  |  ✔  |  ✔  |  506506 ​ | +|  20x  |  HC PL FLUOTAR ​ |  Air  |  0.50  |   ​1.15 ​ |  ✔  |  ✔  |  ✔  |  ✔  |  506506 ​ | 
-|  20x  |  HC PLAN APO  |  Air  |  0.70  |  0.59  ​|  0.53  |  19 pixels ​ | |  ✔  |  ✘  |  ✔  |  ✔  |  506166 ​ | +|  20x  |  HC PLAN APO  |  Air  |  0.70  |  0.59  |  ✔  |  ✘  |  ✔  |  ✔  |  506166 ​ | 
-|  40x  |  HCX PL FLUOTAR ​ |  Air  |  0.75  |  0.40  ​|  0.26  |  39 pixels ​ | |  ✔  |  ✔  |  ✔  |  ✔  |  506145 ​ | +|  40x  |  HCX PL FLUOTAR ​ |  Air  |  0.75  |  0.40  |  ✔  |  ✔  |  ✔  |  ✔  |  506145 ​ | 
-|  63x  |  HCX PL APO  |  Oil  |  1.4-0.60 ​ |  0.10  ​|  0.16  |  63 pixels ​ | |  ✔  |  ✘  |  ✔  |  ✔  |  506187 ​ | +|  63x  |  HCX PL APO  |  Oil  |  1.4-0.60 ​ |  0.10  |  ✔  |  ✘  |  ✔  |  ✔  |  506187 ​ |
- +
-*** pixel size for 1300 x 1030 images**+
  
 ---- ----
-[[resources|Back to the Resources ​page]]+[[resources|Back to the Equipment ​page]]
leica_dm5000b.1583059875.txt.gz · Last modified: 2020/03/01 11:51 by bioimaging