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leica_dm5000b [2019/10/31 12:37]
bioimaging [Objectives]
leica_dm5000b [2020/06/01 19:26] (current)
bioimaging
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-<img src="/​facility/​bioimaging/​lib/​exe/​fetch.php?​media=leica_dm5000b_name.jpg">+<img src="/​facility/​bioimaging/​lib/​exe/​fetch.php?​media=leica_dm5000b_name_new.png">
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-<a href="/​facility/​bioimaging/​lib/​exe/​fetch.php?​media=leica_dm5000b_wiki.jpg"><​img src="/​facility/​bioimaging/​lib/​exe/​fetch.php?​media=leica_dm5000b_wiki.jpg" width=300></​a>​+<a href="/​facility/​bioimaging/​lib/​exe/​fetch.php?​media=leica_dm5000b_wiki_new.png"><​img src="/​facility/​bioimaging/​lib/​exe/​fetch.php?​media=leica_dm5000b_wiki_new.png" width=300></​a>​
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-<​b>​Location</​b>:​ <a href="/​facility/​bioimaging/​lib/​exe/​fetch.php?​media=leica_dm5000b_map.jpg">​Room P0-B-32</​a><​br> ​+<​b>​Location</​b>:​ <a href="/​facility/​bioimaging/​lib/​exe/​fetch.php?​media=leica_dm5000b_map_new.png">​Room P0-B-32</​a><​br> ​
 <​b>​Manufacturer</​b>:​ <a href="​http://​www.leica-microsystems.com/​home/"​ target="​_blank">​Leica Microsystems</​a><​br>​ <​b>​Manufacturer</​b>:​ <a href="​http://​www.leica-microsystems.com/​home/"​ target="​_blank">​Leica Microsystems</​a><​br>​
 <​b>​Model</​b>:​ <a href="​http://​www.leica-microsystems.com/​products/​light-microscopes/​life-science-research/​upright-microscopes/​details/​product/​leica-dm5000-b/"​ target="​_blank">​DM5000 B</​a><​br>​ <​b>​Model</​b>:​ <a href="​http://​www.leica-microsystems.com/​products/​light-microscopes/​life-science-research/​upright-microscopes/​details/​product/​leica-dm5000-b/"​ target="​_blank">​DM5000 B</​a><​br>​
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 &nbsp <br> &nbsp <br>
 &nbsp <br> &nbsp <br>
-&rarr; <a href="​https://​imm.medicina.ulisboa.pt/intranet/booking/9"><​img src="/​facility/​bioimaging/​lib/​exe/​fetch.php?​media=date.png"> ​ Leica DM5000B Booking</​a>​+&nbsp <​br>​ 
 +&rarr; <a href="​https://​my.agendo.science/calendar/?​id=MzJjYWxlbmRhcnM="><​img src="/​facility/​bioimaging/​lib/​exe/​fetch.php?​media=date.png"> ​ Leica DM5000B Booking</​a>​
  <​br> ​  <​br> ​
 &rarr; <a href="/​facility/​bioimaging/​doku.php?​id=leica_dm5000b_usage"><​img src="/​facility/​bioimaging/​lib/​exe/​fetch.php?​media=chart_line.png"> ​ Leica DM5000B Usage Statistics</​a>​ &rarr; <a href="/​facility/​bioimaging/​doku.php?​id=leica_dm5000b_usage"><​img src="/​facility/​bioimaging/​lib/​exe/​fetch.php?​media=chart_line.png"> ​ Leica DM5000B Usage Statistics</​a>​
- <​br>​ +</p>
-&rarr; <a href="/​facility/​bioimaging/​doku.php?​id=leica_dm5000b_repair"><​img src="/​facility/​bioimaging/​lib/​exe/​fetch.php?​media=repair-icon.gif"​ width=16>​ Leica DM5000B Repair History</​a>​ +
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 ===== Microscope overview ===== ===== Microscope overview =====
 +
 The Leica DM5000B is an automated upright //widefield fluorescence microscope//​ ideal for imaging FISH or other fixed fluorescence slides. The microscope is prepared for brightfield,​ phase contrast, DIC and polarization contrast techniques (check objectives list for contrast method availability). It has a monochrome CCD camera for fluorescence image acquisition. If you want to image live samples and/or perform time-lapse experiments check out the [[zeiss_axiovert_200m|Zeiss Axiovert 200M]] or the [[zeiss_cell_observer|Zeiss Cell Observer]] instead. If you need a color camera for brightfield images, try the [[leica_dm2500|Leica DM2500]]. The Leica DM5000B is an automated upright //widefield fluorescence microscope//​ ideal for imaging FISH or other fixed fluorescence slides. The microscope is prepared for brightfield,​ phase contrast, DIC and polarization contrast techniques (check objectives list for contrast method availability). It has a monochrome CCD camera for fluorescence image acquisition. If you want to image live samples and/or perform time-lapse experiments check out the [[zeiss_axiovert_200m|Zeiss Axiovert 200M]] or the [[zeiss_cell_observer|Zeiss Cell Observer]] instead. If you need a color camera for brightfield images, try the [[leica_dm2500|Leica DM2500]].
  
-{{warning.jpg?20|}} Data files older than **3 months** will be automatically deleted on this system, please copy your data to the iMM server using the desktop link. +{{:warning.png?25|}} Data files older than **3 months** will be automatically deleted on this system, please copy your data to the iMM server using the desktop link.
  
 ===== System components ===== ===== System components =====
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 ==== Filtersets ==== ==== Filtersets ====
  
-^  Filterset ​ ^  Reference ​ ^  Excitation ​ ^  Emission ​ ^  Fluorochromes ​ ^ +^  Filterset ​ ^  Reference ​ ^  Excitation ​ ​^ ​ Dichroic ​ ​^ ​ Emission ​ ^  Fluorochromes ​ ^ 
-|  Blue  |  A4  |  ​320-400 nm        |  ​430-510 nm  |  DAPI, Hoechst, Alexa350 ​ | +|  Blue  |  A4  |  ​340-380 nm  |  ​400 nm  |  ​450-490 nm  |  DAPI, Hoechst, Alexa350 ​ | 
-|  Green  |  GFP  |  450-490 nm        |  500-550 nm  |  GFP, Cy2, FITC  | +|  Green  |  GFP  |  450-490 nm  ​| ​ 500 nm  ​|  500-550 nm  |  GFP, FITC, Alexa488 ​ | 
-|  Red  |  ​N21  ​| ​ 515-560 nm        |  > 590 nm  |  Rhodamine, Cy3, Texas Red  |+|  Red  |  ​N2.1  ​| ​ 515-560 nm  ​| ​ 580 nm  ​|  > 590 nm  |  Rhodamine, Cy3, Texas Red  |
  
  
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 ==== Objectives ==== ==== Objectives ====
-^  Magnification ​ ^  Model  ^  ​Type  ​^ ​ NA  ^  WD (mm)  ^  pixel size* (μm)  ^  10 μm =  ​^  BF  ^  PH  ^  DIC  ^  POL  ^ +^  Magnification ​ ^  Model  ^  ​Immersion ​ ​^ ​ NA  ^  WD (mm)  ^  pixel size* (μm)  ^  10 μm =  ^  BF  ^  PH  ^  DIC  ^  POL  ​^ ​ Reference ​ ^ 
-|  5x  |  HC PL FLUOTAR ​ |  ​Dry  ​| ​ 0.15  |  12  |  2.00  |  5 pixels ​ | |  ​Yes  ​|  ​No  ​|  ​No  ​|  ​Yes  | +|  5x  |  HC PL FLUOTAR ​ |  ​Air  ​| ​ 0.15  |  12.00  ​| ​ 2.00  |  5 pixels ​ |  ​✔  ​|  ​✘  ​|  ​✘  ​|  ​✔  ​|  ​506504 ​ | 
-|  10x  |  HC PL FLUOTAR ​ |  ​Dry  ​| ​ 0.30  |  11  |  1.00  |  10 pixels ​ | |  ​Yes  ​|  ​Yes  ​|  ​No  ​|  ​Yes  | +|  10x  |  HC PL FLUOTAR ​ |  ​Air  ​| ​ 0.30  |  11.00  ​| ​ 1.00  |  10 pixels ​ |  ​✔  ​|  ​✔  ​|  ​✘  ​|  ​✔  ​|  ​506507 ​ | 
-|  20x  |  HC PL FLUOTAR ​ |  ​Dry  ​| ​ 0.50  |   ​1.15 ​ |  0.53  |  19 pixels ​ | |  ​Yes  ​|  ​Yes  ​|  ​Yes  ​|  ​Yes  | +|  20x  |  HC PL FLUOTAR ​ |  ​Air  ​| ​ 0.50  |   ​1.15 ​ |  0.53  |  19 pixels ​ |  ​✔  ​|  ​✔  ​|  ​✔  ​|  ​✔  ​|  ​506506 ​ | 
-|  20x  |  HC PLAN APO  |  ​Dry  ​| ​ 0.70  |  0.59  |  0.53  |  19 pixels ​ | |  ​Yes  ​|  ​No  ​|  ​Yes  ​|  ​Yes  | +|  20x  |  HC PLAN APO  |  ​Air  ​| ​ 0.70  |  0.59  |  0.53  |  19 pixels ​ |  ​✔  ​|  ​✘  ​|  ​✔  ​|  ​✔  ​|  ​506166 ​ | 
-|  40x  |  HCX PL FLUOTAR ​ |  ​Dry  ​| ​ 0.75  |  0.40  |  0.26  |  39 pixels ​ | |  ​Yes  ​|  ​Yes  ​|  ​Yes  ​|  ​Yes  | +|  40x  |  HCX PL FLUOTAR ​ |  ​Air  ​| ​ 0.75  |  0.40  |  0.26  |  39 pixels ​ |  ​✔  ​|  ​✔  ​|  ​✔  ​|  ​✔  ​|  ​506145 ​ | 
-|  63x  |  HCX PL APO  |  Oil  |  1.4-0.60 ​ |  0. ​| ​ 0.16  |  63 pixels ​ | |  ​Yes  ​|  ​No  ​|  ​Yes  ​|  ​Yes  |+|  63x  |  HCX PL APO  |  Oil  |  1.4-0.60 ​ |  0.10  ​| ​ 0.16  |  63 pixels ​ |  ​✔  ​|  ​✘  ​|  ​✔  ​|  ​✔  ​|  ​506187 ​ |
  
 *** pixel size for 1300 x 1030 images** *** pixel size for 1300 x 1030 images**
- 
-**Upon request:** 
- 
-^  Magnification ​  ​^ ​ Model  ^  Type  ^  NA  ^  WD (mm)  ^  pixel size* (μm)  ^  10 μm =  ^   ​BF ​ ^  PH  ^  DIC  ^ POL ^ 
-|  100x  |  HCX PL FLUOTAR ​ |  Oil  |  1.3  |  0.13  |  0.10  |  96 pixels ​ |  Yes  |  No  |  Yes  |  Yes  | 
  
 ---- ----
 [[resources|Back to the Resources page]] [[resources|Back to the Resources page]]
leica_dm5000b.1572521846.txt.gz · Last modified: 2019/10/31 12:37 by bioimaging