Bioimaging Wiki

This page is read only. You can view the source, but not change it. Ask your administrator if you think this is wrong.
<html>
<img src="/facility/bioimaging/lib/exe/fetch.php?media=leica_dm5000b_name_new.png">
<table style="border:0px solid white;">
<tr style="border:0px solid white;">
<td style="border:0px solid white;">
<a href="/facility/bioimaging/lib/exe/fetch.php?media=leica_dm5000b_wiki_2022.png"><img src="/facility/bioimaging/lib/exe/fetch.php?media=leica_dm5000b_wiki_2022.png" width=300></a>
<td style="border:0px solid white;"> <p style="line-height:1.8">
<b>Location</b>: <a href="/facility/bioimaging/lib/exe/fetch.php?media=leica_dm5000b_map_new.png">Room P2-A-15</a><br> 
<b>Manufacturer</b>: <a href="http://www.leica-microsystems.com/home/" target="_blank">Leica Microsystems</a><br>
<b>Model</b>: <a href="http://www.leica-microsystems.com/products/light-microscopes/life-science-research/upright-microscopes/details/product/leica-dm5000-b/" target="_blank">DM5000 B</a><br>
<b>Nickname</b>: "5000B"<br>
<b>Year</b>: 2006 <br>
<b>SN</b>: 269835<br>
&nbsp <br>
&nbsp <br>
&nbsp <br>
&nbsp <br>
&nbsp <br>
&rarr; <a href="/facility/bioimaging/doku.php?id=leica_dm5000b_quality"><img src="/facility/bioimaging/lib/exe/fetch.php?media=list-icon.png" width=18> Leica DM5000B Quality Control</a>
 <br>  
&rarr; <a href="/facility/bioimaging/doku.php?id=leica_dm5000b_usage"><img src="/facility/bioimaging/lib/exe/fetch.php?media=chart_line.png">  Leica DM5000B Usage Statistics</a>
</p>
</td>
</tr>
</table>
</html>

===== Microscope overview =====

The Leica DM5000B is an automated upright //widefield fluorescence microscope// ideal for checking FISH or other fixed fluorescence slides. The microscope is prepared for brightfield, phase contrast, DIC and polarization contrast techniques (check objectives list for contrast method availability). If you want to image live samples and/or perform time-lapse experiments check out the [[zeiss_axiovert_200m|Zeiss Axiovert 200M]] or the [[zeiss_cell_observer|Zeiss Cell Observer]] instead. If you need a color camera for brightfield images, try the [[leica_dm2500|Leica DM2500]].

===== System components =====

==== Filtersets ====

^  Filterset  ^  Reference  ^  Excitation  ^  Dichroic  ^  Emission  ^  Fluorochromes  ^
|  Blue  |  A4  |  340-380 nm  |  400 nm  |  450-490 nm  |  DAPI, Hoechst, Alexa350  |
|  Green  |  GFP  |  450-490 nm  |  500 nm  |  500-550 nm  |  GFP, FITC, Alexa488  |
|  Red  |  N2.1  |  515-560 nm  |  580 nm  |  > 590 nm  |  Rhodamine, Cy3, Texas Red  |




==== Objectives ====
^  Magnification  ^  Model  ^  Immersion  ^  NA  ^  WD (mm)  ^  BF  ^  PH  ^  DIC  ^  POL  ^  Reference  ^
|  5x  |  HC PL FLUOTAR  |  Air  |  0.15  |  12.00  |  ✔  |  ✘  |  ✘  |  ✔  |  506504  |
|  10x  |  HC PL FLUOTAR  |  Air  |  0.30  |  11.00  |  ✔  |  ✔  |  ✘  |  ✔  |  506507  |
|  20x  |  HC PL FLUOTAR  |  Air  |  0.50  |   1.15  |  ✔  |  ✔  |  ✔  |  ✔  |  506506  |
|  20x  |  HC PLAN APO  |  Air  |  0.70  |  0.59  |  ✔  |  ✘  |  ✔  |  ✔  |  506166  |
|  40x  |  HCX PL FLUOTAR  |  Air  |  0.75  |  0.40  |  ✔  |  ✔  |  ✔  |  ✔  |  506145  |
|  63x  |  HCX PL APO  |  Oil  |  1.4-0.60  |  0.10  |  ✔  |  ✘  |  ✔  |  ✔  |  506187  |

----
[[resources|Back to the Equipment page]]
Bioimaging Wiki

User Tools

Site Tools

Page Tools
