Bioimaging Wiki

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<b>Location</b>: <a href="/facility/bioimaging/lib/exe/fetch.php?media=leica_dm5000b_map_new.png">Room P0-B-32</a><br> 
<b>Manufacturer</b>: <a href="http://www.leica-microsystems.com/home/" target="_blank">Leica Microsystems</a><br>
<b>Model</b>: <a href="http://www.leica-microsystems.com/products/light-microscopes/life-science-research/upright-microscopes/details/product/leica-dm5000-b/" target="_blank">DM5000 B</a><br>
<b>Nickname</b>: "5000B"<br>
<b>Software</b>: Leica DC Twain<br>
<b>Year</b>: 2006 <br>
<b>SN</b>: 269835<br>
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&rarr; <a href="https://imm.medicina.ulisboa.pt/intranet/booking/9"><img src="/facility/bioimaging/lib/exe/fetch.php?media=date.png">  Leica DM5000B Booking</a>
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&rarr; <a href="/facility/bioimaging/doku.php?id=leica_dm5000b_usage"><img src="/facility/bioimaging/lib/exe/fetch.php?media=chart_line.png">  Leica DM5000B Usage Statistics</a>
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===== Microscope overview =====
The Leica DM5000B is an automated upright //widefield fluorescence microscope// ideal for imaging FISH or other fixed fluorescence slides. The microscope is prepared for brightfield, phase contrast, DIC and polarization contrast techniques (check objectives list for contrast method availability). It has a monochrome CCD camera for fluorescence image acquisition. If you want to image live samples and/or perform time-lapse experiments check out the [[zeiss_axiovert_200m|Zeiss Axiovert 200M]] or the [[zeiss_cell_observer|Zeiss Cell Observer]] instead. If you need a color camera for brightfield images, try the [[leica_dm2500|Leica DM2500]].

{{:warning.png?25|}} Data files older than **3 months** will be automatically deleted on this system, please copy your data to the iMM server using the desktop link.

===== System components =====

==== Filtersets ====

^  Filterset  ^  Reference  ^  Excitation  ^  Emission  ^  Fluorochromes  ^
|  Blue  |  [[https://research.missouri.edu/mcc/files/leica_a4.pdf|A4]]  |  320-400 nm        |  430-510 nm  |  DAPI, Hoechst, Alexa350  |
|  Green  |  GFP  |  450-490 nm        |  500-550 nm  |  GFP, Cy2, FITC  |
|  Red  |  N21  |  515-560 nm        |  > 590 nm  |  Rhodamine, Cy3, Texas Red  |




==== Objectives ====
^  Magnification  ^  Model  ^  Type  ^  NA  ^  WD (mm)  ^  pixel size* (μm)  ^  10 μm =  ^ ^  BF  ^  PH  ^  DIC  ^  POL  ^
|  5x  |  HC PL FLUOTAR  |  Dry  |  0.15  |  12  |  2.00  |  5 pixels  | |  Yes  |  No  |  No  |  Yes  |
|  10x  |  HC PL FLUOTAR  |  Dry  |  0.30  |  11  |  1.00  |  10 pixels  | |  Yes  |  Yes  |  No  |  Yes  |
|  20x  |  HC PL FLUOTAR  |  Dry  |  0.50  |   1.15  |  0.53  |  19 pixels  | |  Yes  |  Yes  |  Yes  |  Yes  |
|  20x  |  HC PLAN APO  |  Dry  |  0.70  |  0.59  |  0.53  |  19 pixels  | |  Yes  |  No  |  Yes  |  Yes  |
|  40x  |  HCX PL FLUOTAR  |  Dry  |  0.75  |  0.40  |  0.26  |  39 pixels  | |  Yes  |  Yes  |  Yes  |  Yes  |
|  63x  |  HCX PL APO  |  Oil  |  1.4-0.60  |  0.1  |  0.16  |  63 pixels  | |  Yes  |  No  |  Yes  |  Yes  |

*** pixel size for 1300 x 1030 images**

**Upon request:**

^  Magnification   ^  Model  ^  Type  ^  NA  ^  WD (mm)  ^  pixel size* (μm)  ^  10 μm =  ^   BF  ^  PH  ^  DIC  ^ POL ^
|  100x  |  HCX PL FLUOTAR  |  Oil  |  1.3  |  0.13  |  0.10  |  96 pixels  |  Yes  |  No  |  Yes  |  Yes  |

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