Bioimaging Wiki

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<a href="/facility/bioimaging/lib/exe/fetch.php?media=leica_dm5000b_wiki.jpg"><img src="/facility/bioimaging/lib/exe/fetch.php?media=leica_dm5000b_wiki.jpg" width=300></a>
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<b>Location</b>: <a href="/facility/bioimaging/lib/exe/fetch.php?media=leica_dm5000b_map.jpg">Room P3-A-44</a><br> 
<b>Manufacturer</b>: <a href="http://www.leica-microsystems.com/home/" target="_blank">Leica Microsystems</a><br>
<b>Model</b>: <a href="http://www.leica-microsystems.com/products/light-microscopes/life-science-research/upright-microscopes/details/product/leica-dm5000-b/" target="_blank">DM5000 B</a><br>
<b>Nickname</b>: "5000B"<br>
<b>Software</b>: Leica DC Twain<br>
<b>Year</b>: 2006 <br>
<b>SN</b>: 269835<br>
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&rarr; <a href="https://imm.medicina.ulisboa.pt/intranet/booking/9"><img src="/facility/bioimaging/lib/exe/fetch.php?media=date.png">  Leica DM5000B Booking</a>
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&rarr; <a href="/facility/bioimaging/doku.php?id=leica_dm5000b_usage"><img src="/facility/bioimaging/lib/exe/fetch.php?media=chart_line.png">  Leica DM5000B Usage Statistics</a>
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&rarr; <a href="/facility/bioimaging/doku.php?id=leica_dm5000b_repair"><img src="/facility/bioimaging/lib/exe/fetch.php?media=repair-icon.gif" width=16> Leica DM5000B Repair History</a>
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===== Microscope overview =====
The Leica DM5000B is an automated upright //widefield fluorescence microscope// ideal for imaging FISH or other fixed fluorescence slides. The microscope is prepared for brightfield, phase contrast, DIC and polarization contrast techniques (check objectives list for contrast method availability). It has a monochrome CCD camera for fluorescence image acquisition. If you want to image live samples and/or perform time-lapse experiments check out the [[zeiss_axiovert_200m|Zeiss Axiovert 200M]] instead. If you need a color camera for brightfield images, try the [[leica_dm2500|Leica DM2500]].

===== System components =====

==== Filtersets ====

^  Filterset  ^  Reference  ^  Excitation  ^  Emission  ^  Fluorochromes  ^
|  Blue  |  A4  |  320-400 nm        |  430-510 nm  |  DAPI, Hoechst, Alexa350  |
|  Green  |  GFP  |  450-490 nm        |  500-550 nm  |  GFP, Cy2, FITC  |
|  Red  |  N21  |  515-560 nm        |  > 590 nm  |  Rhodamine, Cy3, Texas Red  |

==== Objectives ====
^  Magnification  ^  Model  ^  Type  ^  NA  ^  WD (mm)  ^  pixel size* (μm)  ^  10 μm =  ^ ^  BF  ^  PH  ^  DIC  ^  POL  ^
|  5x  |  HC PL FLUOTAR  |  Dry  |  0.15  |  12  |  2.00  |  5 pixels  | |  Yes  |  No  |  No  |  Yes  |
|  10x  |  HC PL FLUOTAR  |  Dry  |  0.30  |  11  |  1.00  |  10 pixels  | |  Yes  |  Yes  |  No  |  Yes  |
|  20x  |  HC PL FLUOTAR  |  Dry  |  0.50  |   1.15  |  0.53  |  19 pixels  | |  Yes  |  Yes  |  Yes  |  Yes  |
|  20x  |  HC PLAN APO  |  Dry  |  0.70  |  0.59  |  0.53  |  19 pixels  | |  Yes  |  No  |  Yes  |  Yes  |
|  40x  |  HCX PL FLUOTAR  |  Dry  |  0.75  |  0.40  |  0.26  |  39 pixels  | |  Yes  |  Yes  |  Yes  |  Yes  |
|  63x  |  HCX PL APO  |  Oil  |  1.4-0.60  |  0.1  |  0.16  |  63 pixels  | |  Yes  |  No  |  Yes  |  Yes  |
|  100x  |  HCX PL FLUOTAR  |  Oil  |  1.3  |  0.13  |  0.10  |  96 pixels  | |  Yes  |  No  |  Yes  |  Yes  |
*** pixel size for 1300 x 1030 images**
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