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Location: Room P0-B-32
Manufacturer: Leica Microsystems
Model: DM5000 B
Nickname: "5000B"
Software: Leica DC Twain
Year: 2006
SN: 269835
Leica DM5000B Booking
Leica DM5000B Usage Statistics

Microscope overview

The Leica DM5000B is an automated upright widefield fluorescence microscope ideal for imaging FISH or other fixed fluorescence slides. The microscope is prepared for brightfield, phase contrast, DIC and polarization contrast techniques (check objectives list for contrast method availability). It has a monochrome CCD camera for fluorescence image acquisition. If you want to image live samples and/or perform time-lapse experiments check out the Zeiss Axiovert 200M or the Zeiss Cell Observer instead. If you need a color camera for brightfield images, try the Leica DM2500.

Data files older than 3 months will be automatically deleted on this system, please copy your data to the iMM server using the desktop link.

System components


Filterset Reference Excitation Emission Fluorochromes
Blue A4 340-380 nm 450-490 nm DAPI, Hoechst, Alexa350
Green GFP 450-490 nm 500-550 nm GFP, FITC, Alexa488
Red N2.1 515-560 nm > 590 nm Rhodamine, Cy3, Texas Red


Magnification Model Immersion NA WD (mm) pixel size* (μm) 10 μm = BF PH DIC POL
5x HC PL FLUOTAR Air 0.15 12 2.00 5 pixels Yes No No Yes
10x HC PL FLUOTAR Air 0.30 11 1.00 10 pixels Yes Yes No Yes
20x HC PL FLUOTAR Air 0.50 1.15 0.53 19 pixels Yes Yes Yes Yes
20x HC PLAN APO Air 0.70 0.59 0.53 19 pixels Yes No Yes Yes
40x HCX PL FLUOTAR Air 0.75 0.40 0.26 39 pixels Yes Yes Yes Yes
63x HCX PL APO Oil 1.4-0.60 0.1 0.16 63 pixels Yes No Yes Yes

* pixel size for 1300 x 1030 images

Upon request:

Magnification Model Type NA WD (mm) pixel size* (μm) 10 μm = BF PH DIC POL
100x HCX PL FLUOTAR Oil 1.3 0.13 0.10 96 pixels Yes No Yes Yes

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leica_dm5000b.txt · Last modified: 2020/01/17 13:05 by bioimaging