Bioimaging Wiki

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<b>Location</b>: <a href="/facility/bioimaging/lib/exe/fetch.php?media=leica_dm2500_map_new.png">Room P3-A-5</a><br> 
<b>Manufacturer</b>: <a href="http://www.leica-microsystems.com/home/" target="_blank">Leica Microsystems</a><br>
<b>Model</b>: DM2500<br>
<b>Nickname</b>: "DM2500"<br>
<b>Software</b>: Leica Application Suite (LAS)<br>
<b>Year</b>: 2006 <br>
<b>SN</b>: 285533-102006<br>
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&rarr; <a href="https://imm.medicina.ulisboa.pt/intranet/booking/10"><img src="/facility/bioimaging/lib/exe/fetch.php?media=date.png">  Leica DM2500 Booking</a>
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&rarr; <a href="/facility/bioimaging/doku.php?id=leica_dm2500_usage"><img src="/facility/bioimaging/lib/exe/fetch.php?media=chart_line.png">  Leica DM2500 Usage Statistics</a>
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===== Microscope overview =====
The Leica DM2500 is an upright //brightfield microscope// equipped with a color camera (Leica DFC450) for brightfield and differential interference contrast (DIC) imaging. If you need to perform fluorescence imaging try the [[leica_dm5000b|Leica DM5000B]] instead.

{{:warning.png?25|}} Data files older than **3 months** will be automatically deleted on this system, please copy your data to the iMM server using the desktop link.
===== System components =====

==== Objectives ====
^  Magnification  ^  Model  ^  Type  ^  NA  ^  WD (mm)  ^ pixel size (μm) ^ 10 μm = ^
|  1.25x  |  HCX PL FLUOTAR  |  Dry  |  0.04  |  3.70  |  4.9  |  2.02 pixels  |
|  10x  |  HC PL FLUOTAR  |  Dry  |  0.30  |  11  |  0.61  |  16.2 pixels  |
|  20x  |  HC PL FLUOTAR  |  Dry  |  0.50  |   1.15  |  0.30  |  32.5 pixels  |
|  40x  |  HCX PL FLUOTAR  |  Dry  |  0.75  |  0.40  |  0.15  |  65 pixels  |
|  100x  |  HCX PL FLUOTAR  |  Oil  |  1.30  |  0.22  |  0.06  |  162.5 pixels  |
*** pixel size for 2560 x 1920 images (Full Frame)**


**Upon request:**

^  Magnification  ^  Model  ^  Type  ^  NA  ^  WD (mm)  ^ pixel size (μm) ^ 10 μm = ^
|  5x  |  N PLAN  |  Dry  |  0.12  |  14  |  1.23  |  8.1 pixels  |
|  63x  |  HCX PL APO  |  Oil  |  1.32-0.60  |  0.1  |  0.1  |  102 pixels  |

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