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leica_dm2500 [2018/10/20 12:35]
bioimaging
leica_dm2500 [2024/01/28 13:46]
bioimaging
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-<img src="/​facility/​bioimaging/​lib/​exe/​fetch.php?​media=leica_dm2500_name.jpg">+<img src="/​facility/​bioimaging/​lib/​exe/​fetch.php?​media=leica_dm2500_name_new.png">
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-<a href="/​facility/​bioimaging/​lib/​exe/​fetch.php?​media=leica_dm2500_wiki.jpg"><​img src="/​facility/​bioimaging/​lib/​exe/​fetch.php?​media=leica_dm2500_wiki.jpg" width=300></​a>​+<a href="/​facility/​bioimaging/​lib/​exe/​fetch.php?​media=leica_dm2500_wiki_2022.png"><​img src="/​facility/​bioimaging/​lib/​exe/​fetch.php?​media=leica_dm2500_wiki_2022.png" width=300></​a>​
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-<​b>​Location</​b>:​ <a href="/​facility/​bioimaging/​lib/​exe/​fetch.php?​media=leica_dm2500_map.jpg">​Room P3-A-7</​a> ​(<img src="/​facility/​bioimaging/​lib/​exe/​fetch.php?​media=telephone_icon.gif"​ width=15>​ 47316)<​br> ​+<​b>​Location</​b>:​ <a href="/​facility/​bioimaging/​lib/​exe/​fetch.php?​media=leica_dm2500_map_new.png">​Room P3-B-41A</​a><​br> ​
 <​b>​Manufacturer</​b>:​ <a href="​http://​www.leica-microsystems.com/​home/"​ target="​_blank">​Leica Microsystems</​a><​br>​ <​b>​Manufacturer</​b>:​ <a href="​http://​www.leica-microsystems.com/​home/"​ target="​_blank">​Leica Microsystems</​a><​br>​
 <​b>​Model</​b>:​ DM2500<​br>​ <​b>​Model</​b>:​ DM2500<​br>​
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 <​b>​Year</​b>:​ 2006 <br> <​b>​Year</​b>:​ 2006 <br>
 <​b>​SN</​b>:​ 285533-102006<​br>​ <​b>​SN</​b>:​ 285533-102006<​br>​
 +&nbsp <br>
 +Data will be deleted after: <b>3 months</​b>​
 +&nbsp <br>
 &nbsp <br> &nbsp <br>
 &nbsp <br> &nbsp <br>
 &nbsp <br> &nbsp <br>
-&rarr; <a href="​https://​imm.medicina.ulisboa.pt/​intranet/​booking/​10"><​img src="/​facility/​bioimaging/​lib/​exe/​fetch.php?​media=date.png"> ​ Leica DM2500 Booking</​a>​ 
- <​br> ​ 
 &rarr; <a href="/​facility/​bioimaging/​doku.php?​id=leica_dm2500_usage"><​img src="/​facility/​bioimaging/​lib/​exe/​fetch.php?​media=chart_line.png"> ​ Leica DM2500 Usage Statistics</​a>​ &rarr; <a href="/​facility/​bioimaging/​doku.php?​id=leica_dm2500_usage"><​img src="/​facility/​bioimaging/​lib/​exe/​fetch.php?​media=chart_line.png"> ​ Leica DM2500 Usage Statistics</​a>​
- <​br>​ +</p>
-&rarr; <a href="/​facility/​bioimaging/​doku.php?​id=leica_dm2500_repair"><​img src="/​facility/​bioimaging/​lib/​exe/​fetch.php?​media=repair-icon.gif"​ width=16>​ Leica DM2500 Repair History</​a>​ +
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 ===== Microscope overview ===== ===== Microscope overview =====
-The Leica DM2500 is an upright //​brightfield microscope//​ equipped with a color camera for brightfield and differential interference contrast (DIC) imaging. If you need to perform fluorescence imaging try the [[leica_dm5000b|Leica DM5000B]] instead.+The Leica DM2500 is an upright //​brightfield microscope//​ equipped with a color camera ​([[https://​www.leica-microsystems.com/​products/​microscope-cameras/​p/​leica-dfc450/​|Leica DFC450]]) ​for brightfield and differential interference contrast (DIC) imaging.
  
-{{warning.jpg?20|}} Data files older than **3 months** will be automatically deleted on this system, please copy your data to the iMM server using the desktop link.+{{:warning.png?25|}} Data files older than **3 months** will be automatically deleted on this system, please copy your data to the iMM server using the desktop link.
 ===== System components ===== ===== System components =====
  
 ==== Objectives ==== ==== Objectives ====
-^  Magnification ​ ^  Model  ^  Type  ^  NA  ^  WD (mm)  ^ pixel size (μm) ^ 10 μm = ^ +^  Magnification ​ ^  Model  ^  Type  ^  NA  ^  WD (mm)  ^  pixel size (μm)   10 μm =  ​^ ​ Reference  ​
-|  1.25x  |  HCX PL FLUOTAR ​ |  Dry  |  0.04  |  3.70  |  4. ​| ​ 2.02 pixels ​ | +|  1.25x  |  HCX PL FLUOTAR ​ |  Dry  |  0.04  |  3.70  |  4.90  ​| ​ 2.pixels ​ ​| ​ 506215 ​ | 
-|  5x  |  N PLAN  |  Dry  |  0.12  |  14  |  1.23  |  8.1 pixels ​ | +|  5x  |  N PLAN  |  Dry  |  0.12  |  14.00  ​| ​ 1.23  |  8.1 pixels ​ ​| ​ 506302 ​ | 
-|  10x  |  HC PL FLUOTAR ​ |  Dry  |  0.30  |  11  |  0.61  |  16.2 pixels ​ | +|  10x  |  HC PL FLUOTAR ​ |  Dry  |  0.30  |  11.00  ​| ​ 0.61  |  16.2 pixels ​ ​| ​ 506507 ​ | 
-|  20x  |  HC PL FLUOTAR ​ |  Dry  |  0.50  |   ​1.15 ​ |  0.30  |  32.5 pixels ​ | +|  20x  |  HC PL FLUOTAR ​ |  Dry  |  0.50  |   ​1.15 ​ |  0.30  |  32.5 pixels ​ ​| ​ 506506 ​ | 
-|  40x  |  HCX PL FLUOTAR ​ |  Dry  |  0.75  |  0.40  |  0.15  |  65 pixels ​ | +|  40x  |  HCX PL FLUOTAR ​ |  Dry  |  0.75  |  0.40  |  0.15  |  65 pixels ​ ​| ​ 506144 ​ | 
-|  100x  |  HCX PL FLUOTAR ​ |  Oil  |  1.30  |  0.22  |  0.06  |  162.5 pixels ​ | +|  100x  |  HCX PL FLUOTAR ​ |  Oil  |  1.30  |  0.22  |  0.06  |  162.5 pixels ​ |  ​506195 ​ |
-*** pixel size for 2560 x 1920 images (Full Frame)**+
  
- +*** pixel size for 2560 x 1920 images (Full Frame)** 
-**Upon request:** +==== Camera ==== 
- +^  Model  ^  Type  ^  ​Frame Size  ​^  ​Pixel Size (µm)  ^ 
-^  Magnification  ​^  Model  ^  Type  ^  ​NA  ​^  ​WD (mm)  ​^ pixel size (μm) ^ 10 μm = +|  ​[[https://​www.leica-microsystems.com/​products/​microscope-cameras/​p/​leica-dfc450-c/​|Leica DFC450]] ​ ​|  ​color CCD  ​|  ​2560 × 1920  ​|  ​3.4 x 3. |
-|  ​63x  ​ HCX PL APO  ​|  ​Oil  ​|  ​1.32-0.60 ​ ​|  ​0.1  |  0.1  |  102 pixels ​ |+
  
 ---- ----
-[[resources|Back to the Resources ​page]]+[[resources|Back to the Equipment ​page]]
leica_dm2500.txt · Last modified: 2024/03/19 12:36 by bioimaging