ivis_lumina
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ivis_lumina [2019/01/11 18:37] bioimaging |
ivis_lumina [2023/03/27 13:09] (current) bioimaging |
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| <html> | <html> | ||
| - | <img src="/facility/bioimaging/lib/exe/fetch.php?media=ivis_lumina_name.jpg"> | + | <img src="/facility/bioimaging/lib/exe/fetch.php?media=ivis_lumina_name_new.png"> |
| <table style="border:0px solid white;"> | <table style="border:0px solid white;"> | ||
| <tr style="border:0px solid white;"> | <tr style="border:0px solid white;"> | ||
| <td style="border:0px solid white;"> | <td style="border:0px solid white;"> | ||
| - | <a href="/facility/bioimaging/lib/exe/fetch.php?media=ivis_lumina_wiki.jpg"><img src="/facility/bioimaging/lib/exe/fetch.php?media=ivis_lumina_wiki.jpg" width=300></a> | + | <a href="/facility/bioimaging/lib/exe/fetch.php?media=ivis_lumina_wiki_2022.png"><img src="/facility/bioimaging/lib/exe/fetch.php?media=ivis_lumina_wiki_2022.png" width=300></a> |
| <td style="border:0px solid white;"> <p style="line-height:1.8"> | <td style="border:0px solid white;"> <p style="line-height:1.8"> | ||
| - | <b>Location</b>: <a href="/facility/bioimaging/lib/exe/fetch.php?media=ivis_lumina_map.jpg">Room P2-A-3</a> (<img src="/facility/bioimaging/lib/exe/fetch.php?media=telephone_icon.gif" width=15> 47200) <br> | + | <b>Location</b>: <a href="/facility/bioimaging/lib/exe/fetch.php?media=ivis_lumina_map_new.png">Room P2-A-3</a> (<img src="/facility/bioimaging/lib/exe/fetch.php?media=phone_neg.png" width=15> 47200) <br> |
| <b>Manufacturer</b>: <a href="http://www.perkinelmer.com/corporate/what-we-do/markets/life-sciences" target="_blank">Perkin Elmer</a> (Caliper LifeSciences)<br> | <b>Manufacturer</b>: <a href="http://www.perkinelmer.com/corporate/what-we-do/markets/life-sciences" target="_blank">Perkin Elmer</a> (Caliper LifeSciences)<br> | ||
| <b>Model</b>: IVIS Lumina<br> | <b>Model</b>: IVIS Lumina<br> | ||
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| <b>SN</b>: IS0715N4196<br> | <b>SN</b>: IS0715N4196<br> | ||
|   <br> |   <br> | ||
| + | Data will be deleted after: <b>3 months</b> | ||
|   <br> |   <br> | ||
|   <br> |   <br> | ||
| - | → <a href="https://imm.medicina.ulisboa.pt/intranet/booking/14"><img src="/facility/bioimaging/lib/exe/fetch.php?media=date.png"> IVIS Lumina Booking</a> | + |   <br> |
| - | <br> | + | → <a href="/facility/bioimaging/doku.php?id=ivis_lumina_quality"><img src="/facility/bioimaging/lib/exe/fetch.php?media=list-icon.png" width=18> IVIS Lumina Quality Control</a> |
| - | → <a href="/facility/bioimaging/doku.php?id=ivis_lumina_usage"><img src="/facility/bioimaging/lib/exe/fetch.php?media=chart_line.png"> IVIS Lumina Usage Statistics</a> | + | |
| <br> | <br> | ||
| - | → <a href="/facility/bioimaging/doku.php?id=ivis_lumina_repair"><img src="/facility/bioimaging/lib/exe/fetch.php?media=repair-icon.gif" width=16> IVIS Lumina Repair History</a> | + | → <a href="/facility/bioimaging/doku.php?id=ivis_lumina_usage"><img src="/facility/bioimaging/lib/exe/fetch.php?media=chart_line.png"> IVIS Lumina Usage Statistics</a> |
| - | </p> | + | </p> |
| </td> | </td> | ||
| </tr> | </tr> | ||
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| An adjustable field of view from 5 – 12.5 cm allows imaging of up to 3 mice or a medium size rat and also accommodates standard microtiter plates for //in vitro// imaging. The system includes premium animal handling features such as a heated sample shelf, gas anesthesia connections and manifold. | An adjustable field of view from 5 – 12.5 cm allows imaging of up to 3 mice or a medium size rat and also accommodates standard microtiter plates for //in vitro// imaging. The system includes premium animal handling features such as a heated sample shelf, gas anesthesia connections and manifold. | ||
| - | {{warning.jpg?20|}} Data files older than **3 months** will be automatically deleted on this system, please copy your data to the iMM server using the desktop link. | + | {{:warning.png?25|}} Data files older than **3 months** will be automatically deleted on this system, please copy your data to the iMM server using the desktop link. |
| + | |||
| + | ===== System booking rules ===== | ||
| + | |||
| + | {{:warning.png?25|}} You can book the system with **30 days** in advance. | ||
| ==== Filtersets ==== | ==== Filtersets ==== | ||
| - | ^ Filter Set ^ Label ^ Background Passband (nm) ^ Excitation Passband (nm) ^ Emission Passband (nm) ^ Dyes, Fluorescent Proteins, and Quantum Dots ^ | + | ^ Filter Set ^ Label ^ Background Passband (nm) ^ Excitation Passband (nm) ^ Emission Passband (nm) ^ Dyes / Fluorescent Proteins ^ |
| | 1 | Green | 410–440 | 445–490 | 515–575 | GFP, EGFP, FITC | | | 1 | Green | 410–440 | 445–490 | 515–575 | GFP, EGFP, FITC | | ||
| - | | 2 | Red | 460–490 | 500–550 | 575–650 | DsRed, PKH26, Qdot® 605 | | + | | 2 | Red | 460–490 | 500–550 | 575–650 | DsRed, PKH26 | |
| - | | 3 | Far-red | 580–610 | 615–665 | 695–770 | Cy5.5, Qdot® 705 | | + | | 3 | Far-red | 580–610 | 615–665 | 695–770 | Cy5.5 | |
| - | | 4 | NIR | 665–695 | 705–780 | 810–885 | ICG, Qdot® 800 | | + | | 4 | NIR | 665–695 | 705–780 | 810–885 | ICG | |
| ===== System Turn On Procedures ===== | ===== System Turn On Procedures ===== | ||
| - | * The system and computer should be turned on. If not please contact the [[imm-bioimaging@medicina.ulisboa.pt|Bioimaging Facility]]. | + | * The system and computer should be turned on. If not please contact the [[imm-bioimaging@medicina.ulisboa.pt|Bioimaging Unit]]. |
| * Check if the light source power supply is turned on (small box between the black box and the monitor). | * Check if the light source power supply is turned on (small box between the black box and the monitor). | ||
| * If the computer shows the login screen, use the username ''administrator'' and password ''password''. | * If the computer shows the login screen, use the username ''administrator'' and password ''password''. | ||
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| * Also clean with ethanol and paper tissues any other place (except the camera lenses or any optical components) that might have been in contact with tissues or fluids. | * Also clean with ethanol and paper tissues any other place (except the camera lenses or any optical components) that might have been in contact with tissues or fluids. | ||
| - | For a complete shutdown contact the [[imm-bioimaging@medicina.ulisboa.pt|Bioimaging Facility]]. | + | For a complete shutdown contact the [[imm-bioimaging@medicina.ulisboa.pt|Bioimaging Unit]]. |
| ===== Imaging Basics ===== | ===== Imaging Basics ===== | ||
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| ---- | ---- | ||
| - | [[resources|Back to the Resources page]] | + | [[resources|Back to the Equipment page]] |
ivis_lumina.1547228275.txt.gz · Last modified: 2019/01/11 18:37 by bioimaging
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