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Table of Contents
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Location: [Oeiras] Room 0B05 |
Andor Spinning Disk W1 Quality Control
Andor Spinning Disk W1 Usage StatisticsMicroscope overview
The Andor Spinning W1 system is a spinning disk laser confocal microscope with a sensitive EMCCD camera suitable for fast in-vivo imaging. It's one of the fastest and most sensitive system at the facility. The system can also scan multiple positions in the sample, with multiple channels in time-lapse mode. In order to stay in focus it also includes a perfect focus system to correct for vertical drift (Nikon's “PFS”) during long movies. Note that this system does not have widefield fluorescence, so samples can only be previewed in brightfield mode.
System components
Lasers
| Type | Excitation line | Nominal power |
|---|---|---|
| Diode405 | 405nm | 100mW |
| DPSS488 | 488nm | 100mW |
| DPSS561 | 561nm | 50mW |
| Diode640 | 640nm | 100mW |
Objectives
| Magnification | Model | Immersion | NA | WD (mm) | PFS compatible | DIC compatible | PhC compatible | Reference |
|---|---|---|---|---|---|---|---|---|
| 4x | Nikon Plan DL | - | 0.13 | 16.5 | N | N/A | Ph1 | MRH20045 |
| 10x | Nikon Plan DL | - | 0.25 | 10.5 | Yes | Not recommended | Ph1 | MRL20102 |
| 20x | Nikon Plan Fluor | - | 0.5 | 2.1 | Yes | Not recommended | Ph1 | MRH10205 |
| 20x | Nikon Plan Fluor | MImm | 0.75 | 0.35 | No | Yes (N1) | No | MRH07241 |
| 40x | Plan Fluor | Oil | 1.3 | 0.24 | Yes | Yes (N2) | No | MRH01401 |
| 63x | Nikon Plan APO VC | W | 1.20 | 0.175 | No | No | No | MRD07602 |
* Not fabricated anymore ; * * LWD - Long Working Distance
CSU Dichroics
| Position | Name/reflection | Transmission |
|---|---|---|
| 1 | IR mirror | 685LP |
| 2 | LD 405/488/561/640 | Quad mirror 80-85% transmission |
| 3 | ZT 405/488/561/640 | Quad mirror >95% transmission |
Filters (CSU Filterwheel)
| Position | Name/Description | Transmission | Dichroic |
|---|---|---|---|
| 1 | Quad Semrock | 440/521/607/700-25 | 2 or 3[HE] |
| 2 | DAPI Semrock | 447/60 | 2 or 3[HE] |
| 3 | GFP Semrock | 525/30 | 2 or 3[HE] |
| 4 | RFP Semrock | 607/36 | 2 or 3[HE] |
| 5 | Cy5 Semrock | 685/40 | 2 or 3[HE] |
| 6 | Empty | - | - |
| 7 | Cy5LP Chroma HE | 655 LP | 2 or 3[HE] |
| 8 | RFP Chroma HE | 595/50 | 2 or 3[HE] |
| 9 | GFP Chroma HE | 525/50 | 2 or 3[HE] |
| 10 | DAPI Chroma HE | 450/50 | 2 or 3[HE] |
Camera(s)
| Model/type | Chip Size | Dexel Size | Quantum Efficiency |
|---|---|---|---|
| Andor iXon 888 | 1024 × 1024 | 13 um | >95% |
| Andor Zyla 4.2 PLUS /sCMOS | 2048 × 2048 | 6.5 um | 82% |
| IDS Monochrome UI-3370CP-M-GL | 2048 × 2048 | 5.5 um | 60% |
<b>Piezo Z Stage</b>: <a href=“https://www.physikinstrumente.com/product-detail-page/p-737-201546.html” target=“_blank”>PI 737.2SL</a> - range: 220µm - Resolution: 1 nm <br>
